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AB125047

Anti-CD68 antibody

4

(9 Reviews)

|

(33 Publications)

Rabbit Polyclonal CD68 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 33 publications.

View Alternative Names

CD68, Macrosialin, Gp110

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (AB125047)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (AB125047)

ab125047, at 1/100, staining CD68 in formalin fixed, paraffin embedded Human tonsil tissue by Immunohistochemistry.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (AB125047)
  • IHC-P

AbReview38278****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (AB125047)

ab125047 staining CD68 in Mouse vasculature tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 5 minutes at 20°C; antigen retrieval was by heat mediation in a tris buffer. Samples were incubated with primary antibody (1/100 in antibody diluent) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Western blot - Anti-CD68 antibody (AB125047)
  • WB

Unknown

Western blot - Anti-CD68 antibody (AB125047)

All lanes:

Western blot - Anti-CD68 antibody (ab125047) at 1/50 dilution

All lanes:

SH SY5Y cell line lysate

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

The user must validate any other storage conditions.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.
Biological function summary

CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.

Pathways

CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.

CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
See full target information CD68
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Publications (33)

Recent publications for all applications. Explore the full list and refine your search

Hepatology communications 9: PubMed40304567

2025

Cellular communication networks in fibrosis: Insights from the MASLD pig model.

Applications

Unspecified application

Species

Unspecified reactive species

Kaiyi Zhang,Jiakun Miao,Juan Du,Yu Yang,Boce Xia,Huanqi Peng,Shuang Xu,Jiangao Fan,Yanfang Wang,Martine Schroyen,Shulin Yang

Cellular and molecular biology (Noisy-le-Grand, France) 70:197-204 PubMed38430022

2024

Homocysteine modulates CXCL10/CXCR3 axis activity to induce endothelial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Yanjie Xu,Yingying Xu,Zuozong Yu,Dingwen He

Heliyon 10:e23498 PubMed38223729

2024

Expression of PD-1 mitigates phagocytic activities TAM in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chenhong Zheng,Heng Li,Xiaohui Zhao,Siyu Yang,Jinqin Zhan,Huaie Liu,Yan Jiang,Li Shi,Yaxian Song,Yujie Lei,Tingdong Yu,Xiaoxiong Wang,Hongsheng Li,Xi Wang,Yushan Xu,Zhihong Yao

Heliyon 9:e21893 PubMed38034686

2023

SARS-CoV-2 activates the TLR4/MyD88 pathway in human macrophages: A possible correlation with strong pro-inflammatory responses in severe COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Sabina Sahanic,Richard Hilbe,Christina Dünser,Piotr Tymoszuk,Judith Löffler-Ragg,Dietmar Rieder,Zlatko Trajanoski,Anne Krogsdam,Egon Demetz,Maria Yurchenko,Christine Fischer,Michael Schirmer,Markus Theurl,Daniela Lener,Jakob Hirsch,Johannes Holfeld,Can Gollmann-Tepeköylü,Carl P Zinner,Alexandar Tzankov,Shen-Ying Zhang,Jean-Laurent Casanova,Wilfried Posch,Doris Wilflingseder,Guenter Weiss,Ivan Tancevski

Pathology, research and practice 251:154901 PubMed37922722

2023

PTX3 shapes profibrotic immune cells and epithelial/fibroblast repair and regeneration in a murine model of pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Antonio d'Amati,Roberto Ronca,Federica Maccarinelli,Marta Turati,Loredana Lorusso,Michelina De Giorgis,Roberto Tamma,Domenico Ribatti,Tiziana Annese

Neural regeneration research 18:1990-1998 PubMed36926724

2023

Low-temperature 3D-printed collagen/chitosan scaffolds loaded with exosomes derived from neural stem cells pretreated with insulin growth factor-1 enhance neural regeneration after traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Yin Liu,Yin-He Feng,Qing-Bo Feng,Jian-Yong Zhang,Lin Zhong,Peng Liu,Shan Wang,Yan-Ruo Huang,Xu-Yi Chen,Liang-Xue Zhou

American journal of physiology. Lung cellular and molecular physiology 324:L584-L595 PubMed36880658

2023

A proinflammatory long noncoding RNA Lncenc1 regulates inflammasome activation in macrophage.

Applications

Unspecified application

Species

Unspecified reactive species

Yohan Han,Yin Zhu,Saugata Dutta,Sultan Almuntashiri,Xiaoyun Wang,Duo Zhang

PloS one 18:e0280385 PubMed36662769

2023

Co-culture models of endothelial cells, macrophages, and vascular smooth muscle cells for the study of the natural history of atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Liu,Saurabhi Samant,Charu Hasini Vasa,Ryan M Pedrigi,Usama M Oguz,Sangjin Ryu,Timothy Wei,Daniel R Anderson,Devendra K Agrawal,Yiannis S Chatzizisis

Molecular therapy : the journal of the American Society of Gene Therapy 31:1346-1364 PubMed36635966

2023

Extracellular vesicle-encapsulated CC16 as novel nanotherapeutics for treatment of acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yohan Han,Yin Zhu,Sultan Almuntashiri,Xiaoyun Wang,Payaningal R Somanath,Caroline A Owen,Duo Zhang

Molecular medicine (Cambridge, Mass.) 28:152 PubMed36510147

2022

Deficiency of mindin reduces renal injury after ischemia reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Bai,Xiong Wang,Cong Qin,Kang Yang,Zhiguo Duan,Zhixiu Cao,Jiaqian Liang,Lei Wang,Jingdong Yuan,Pengcheng Luo
View all publications
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