Anti-CD68 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (AB125212)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CD68 with ab125212 at 2 µg/ml. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with ab125212 overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using a HRP conjugated Rabbit IgG with DAB as the chromogen.

• IHC-P

AbReview46192****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (AB125212)

ab125212 staining CD68 in Mouse skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 30 minutes at 24°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in 10% goat serum) for 16 hours at 4°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview.

• IHC-P

AbReview29942****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (AB125212)

ab125212 staining CD68 in Mouse spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in PBS + 2% BSA + 10% FCS) for 45 minutes at 20°C. A HRP-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (AB125212)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling CD68 with ab125212 at 2 µg/ml. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with ab125212 overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using a HRP conjugated Rabbit IgG with DAB as the chromogen.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (AB125212)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CD68 with ab125212 at 2 µg/ml. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with ab125212 overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using a HRP conjugated Rabbit IgG with DAB as the chromogen.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (AB125212)

Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling CD68 with ab125212 at 2 µg/ml. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with ab125212 overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using a HRP conjugated Rabbit IgG with DAB as the chromogen.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (AB125212)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD68 with ab125212 at 2 µg/ml. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with ab125212 overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using a HRP conjugated Rabbit IgG with DAB as the chromogen.

• IHC-P

AbReview33578****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (AB125212)

Immunohistochemical analysis of murine spleen tissue, staining CD68 with ab125212.

Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (undiluted) for 16 hours at 4°C. An undiluted HRP-conjugated horse anti-rat polyclonal IgG was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (AB125212)

Immunohistochemistry of microglial marker in mouse brain

Cortical staining for the microglial marker, CD68 increased as a function of age in rTg4510 animals, but remained unchanged in tTA animals. Scale bar, 200 μm, inset scale bar, 50 μm.

(After Figure 10 A of Wes et al.)

Wes, P.D. PLoS One. 2014 Aug 25;9(8):e106050. doi: 10.1371/journal.pone.0106050. eCollection 2014 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• WB

Supplier Data

Western blot - Anti-CD68 antibody (AB125212)

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with ab125212 at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at 1/5000 dilution for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD68 at approximately 90-100 kDa.

All lanes:

Western blot - Anti-CD68 antibody (ab125212) at 0.5 µg/mL

Lane 1:

Rat spleen tissue lysates

Lane 2:

Mouse spleen tissue lysates

Lane 3:

RAW264.7 whole cell lysates

Secondary

All lanes:

Goat anti-rabbit IgG-HRP at 1/5000 dilution

false

• WB

Lab

Western blot - Anti-CD68 antibody (AB125212)

False colour image of Western blot : Anti-CD68 antibody staining at 0.2 μg/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab125212 was shown to bind specifically to CD68. A band was observed at 95-102 kDa in wild-type RAW 264.7 cell lysates with no signal observed at this size in CD68 knockout cell line ab280047 (knockout cell lysate ab280106). To generate this image, wild-type and CD68 knockout RAW 264.7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-CD68 antibody (ab125212) at 0.2 µg/mL

Lane 1:

Wild-type RAW 264.7 cell lysate at 20 µg

Lane 2:

CD68 knockout RAW 264.7 cell lysate at 20 µg

Lane 2:

Western blot - Mouse CD68 knockout RAW 264.7 cell line (<a href='/en-us/products/cell-lines/mouse-cd68-knockout-raw-2647-cell-line-ab280047'>ab280047</a>)

Lane 3:

Mouse spleen cell lysate at 20 µg

Lane 4:

Neuro-2a cell lysate at 20 µg

Predicted band size: 37 kDa

Observed band size: 95-102 kDa

false

• WB

CiteAb

Western blot - Anti-CD68 antibody (AB125212)

CD68 western blot using anti-CD68 antibody ab125212. Publication image and figure legend from Guido, M. C., Marques, A. F., et al., 2017, Oxid Med Cell Longev, PubMed 28781721.

ab125212 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab125212 please see the product overview.

(a) Total inflammatory cell count in SEN and INT areas. HE-stained sections, under 400x magnification. θp < 0.05 versus the DM SEN area; *p < 0.01 versus the CT SEN area, #p < 0.01 versus the DM SEN area; †p < 0.01 versus the CT INT area. Data expressed in mean ± SEM. Western blot protein expression analysis depicting CD68 (b), CD3 (c), MCP-1 (d), TNF-α (e), IL1-β (f), and IL-6 (g) was performed 8 weeks after diabetes induction. *p < 0.05 and †p < 0.001 versus the CT group. Data expressed in mean ± SEM in all plots.

false