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AB201340

Anti-CD68 antibody [C68/684]

5

(3 Reviews)

|

(149 Publications)

Anti-CD68 antibody [C68/684] (ab201340) is a mouse monoclonal antibody detecting CD68 in Western Blot, Flow Cytometry, IHC-P, ICC/IF. Suitable for Human.

- Over 100 publications

View Alternative Names

CD68, Macrosialin, Gp110

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [C68/684] (AB201340)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [C68/684] (AB201340)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD68 with ab201340 at 1 μg/ml.

Flow Cytometry - Anti-CD68 antibody [C68/684] (AB201340)
  • Flow Cyt

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Flow Cytometry - Anti-CD68 antibody [C68/684] (AB201340)

Flow Cytometry analysis of U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells labeling CD68 with ab201340. Goat Anti-Mouse IgG was used as the secondary antibody (blue); Isotype Control (red).

Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [C68/684] (AB201340)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [C68/684] (AB201340)

Immunocytochemistry/immunofluorescence analysis of U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells labelling CD68 with ab201340. Goat Anti-Mouse IgG was used as the secondary antibody (green). Nuclear DNA stained red.

Western blot - Anti-CD68 antibody [C68/684] (AB201340)
  • WB

Supplier Data

Western blot - Anti-CD68 antibody [C68/684] (AB201340)

All lanes:

Western blot - Anti-CD68 antibody [C68/684] (ab201340)

All lanes:

Human spleen tissue lysate

Predicted band size: 37 kDa

Observed band size: 110 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

C68/684

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CD68.

P34810

Specificity

ab201340 recognizes a glycoprotein of 110kDa, which is identified as CD68.

Reactivity data

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Product details

Anti-CD68 antibody [C68/684] (ab201340) is a House Mouse Monoclonal antibody and is validated for use in Flow Cyt, ICC/IF, IHC-P, WB in human samples.

Anti-CD68 antibody [C68/684] (ab201340) has been cited over 105 times in peer reviewed journals and is trusted by the scientific community.

Abcams high quality validation processes ensure Anti-CD68 antibody [C68/684] (ab201340) has high sensitivity and specificity.

Anti-CD68 antibody [C68/684] (ab201340) specifically detects CD68 (UniProt ID: P34810; Molecular weight: 35kDa) and is sold in 100 uL selling sizes.

Conjugation-ready, carrier free format available for antibody clone C68/684 - ab21398.

CD68 is a marker primarily associated with macrophages, playing a crucial role in phagocytosis, antigen presentation, and immune response regulation. CD68 is a biomarker in chronic inflammatory conditions including rheumatoid arthritis and inflammatory bowel disease

ab201340 stains macrophages in a wide variety of Human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
ab201340 is purified from Bioreactor Concentrate by Protein A/G column chromatography.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.
Biological function summary

CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.

Pathways

CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.

CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
See full target information CD68

Publications (149)

Recent publications for all applications. Explore the full list and refine your search

Mediators of inflammation 2025:4932970 PubMed40918406

2025

Electroacupuncture Attenuates Hepatic Ischemia-Reperfusion Injury by Modulating the Esr1/TAK1-JNK/p38 Signaling Pathway in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofang Fan,Wei Guo,Xiaodan Yang,Hao Zhang,Bruno Fink,Lingyu Hu,Xiaoguang Wang

Journal of translational medicine 23:923 PubMed40826409

2025

Metabolic interplay between endometrial cancer and tumor-associated macrophages: lactate-induced M2 polarization enhances tumor progression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodie Liu,Heming Sun,Jing Liang,Huan Yu,Ming Xue,Yuebo Li,Wenhui Li,Ying Xu,Bin Ling,Dingqing Feng

Regenerative biomaterials 12:rbaf062 PubMed40708704

2025

Two-in-one: multifunctional poloxamer hydrogel accelerates endometrial regeneration and fertility restoration through synergistic regulation of KGF-2 and NO.

Applications

Unspecified application

Species

Unspecified reactive species

Yijia Zhang,Xinji Wang,Qin Gu,Cuitao Lu,Yingzheng Zhao,Xiaokun Li

Frontiers in cell and developmental biology 13:1592594 PubMed40607212

2025

SOX9 mediates the phenotypic transformation of vascular smooth muscle cells in restenosis after carotid artery injury.

Applications

Unspecified application

Species

Unspecified reactive species

Chuan Jiang,Jiasen Ye,Jie Huang,Yang Gao,Hong Chen,Fuqiang Guo,Lei Guo,Xiaofan Yuan

Nature communications 16:3705 PubMed40251193

2025

Covalently reactive microparticles imbibe blood to form fortified clots for rapid hemostasis and prevention of rebleeding.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Chen,Chaonan Xiao,Xianjun Chen,Ziyi Yang,Jingwei Zhao,Bingkun Bao,Qingmei Zeng,Li Jiang,Xinyi Huang,Yi Yang,Qiuning Lin,Wei Gong,Linyong Zhu

Nature communications 16:3283 PubMed40189598

2025

Peptide-based inflammation-responsive implant coating sequentially regulates bone regeneration to enhance interfacial osseointegration.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhou,Yang Liu,Xuan Nie,Chen Zhu,Liming Xiong,Jing Zhou,Wei Huang

Bone research 13:26 PubMed39994205

2025

CCN2 mediates fibroblast-macrophage interaction in knee arthrofibrosis based on single-cell RNA-seq analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyun Li,Jia Jiang,Kangwen Cai,Yi Qiao,Xuancheng Zhang,Liren Wang,Yuhao Kang,Xiulin Wu,Benpeng Zhao,Xiuli Wang,Tianyi Zhang,Zhiqi Lin,Jinlong Wu,Simin Lu,Haihan Gao,Haocheng Jin,Caiqi Xu,Xiaoqiao Huangfu,Zhengzhi James,Qiuhua Chen,Xiaoqi Zheng,Ning-Ning Liu,Jinzhong Zhao

Cell reports. Medicine 6:101932 PubMed39879993

2025

A functional cardiac patch promotes cardiac repair by modulating the CCR2 cardiac-resident macrophage niche and their cell crosstalk.

Applications

Unspecified application

Species

Unspecified reactive species

Chengbin Ding,Guofeng Tang,Yan Sun,Xiaodong Fu,Ye Tian,Jiamian Zhan,Songtao Zhang,Xianglong Xing,Jianing Liu,Xiaozhong Qiu,Leyu Wang

International journal of ophthalmology 18:15-27 PubMed39829614

2025

IL-17A mediates inflammation-related retinal pigment epithelial cells injury ERK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Min Zhong,Bing-Qiao Shen,Yu-Hong Chen,Xiao-Huan Zhao,Xiao-Xu Huang,Min-Wen Zhou,Xiao-Dong Sun

Nature communications 16:52 PubMed39746939

2025

Living plastics from plasticizer-assisted thermal molding of silk protein.

Applications

Unspecified application

Species

Unspecified reactive species

Yushu Wang,Junqi Wu,Emily J Hartzell,Weiguo Hu,Reddhy Mahle,Xinxin Li,Ying Chen,Jugal Kishore Sahoo,Cameron Chan,Brooke N Longo,Charlotte S Jacobus,Chunmei Li,David L Kaplan
View all publications

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