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AB31630

Anti-CD68 antibody [ED1]

5

(21 Reviews)

|

(490 Publications)

Anti-CD68 antibody [ED1] (ab31630) is a mouse monoclonal antibody detecting CD68 in Flow Cytometry (Intra). Suitable for Rat.

- Over 400 publications
- Trusted since 2006
2 Images
Flow Cytometry (Intracellular) - Anti-CD68 antibody [ED1] (AB31630)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CD68 antibody [ED1] (AB31630)

Staining of rat peritoneal macrophages with Alexa Fluor®647 conjugated mouse anti rat CD68, clone ED1 (ab31630, red filled histogram) following permeabilization with Leucoperm.

Flow Cytometry - Anti-CD68 antibody [ED1] (AB31630)
  • Flow Cyt

CiteAb

Flow Cytometry - Anti-CD68 antibody [ED1] (AB31630)

CD68 flow cytometry using Anti-CD68 antibody [ED1] ab31630. IPublication image and figure legend from Xia, Z., Wang, J., et al., 2021, Braz J Med Biol Res, PubMed 34320121.

Emodin modulated in vivo M1 and M2 polarization of macrophages in implanted polyvinyl alcohol (PVA) sponges. A-D, The implanted PVA sponges were removed and assessed for M1 (A and C) and M2 (B and D) populations at 3 days (A and B) and 7 days (C and D) after implantation. The percentages of M1 and M2 macrophages and total macrophages in sponge exudates were determined by flow cytometry using the indicated markers. Representative flow profiles are shown (left), and the percentages of M1/M2 populations are summarized (right). Data are reported as means±SE for n=3 for each group. *P,0.05, **P<0.01, ***P<0.001 vs the Control group (two-tailed t-test).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

ED1

Isotype

IgG1

Carrier free

No

Reacts with

Rat

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab31630 recognises a single chain glycoprotein of 110kD that is expressed predominantly on the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen recognised by ED1 is the rat homologue of human CD68.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50 - 1/100", "FlowCytIntra-species-notes": "<p>Use 10μl of the suggested working dilution to label 10<sup>6</sup> cells in 100μl. <a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Product Specifications

Anti-CD68 antibody [ED1] (ab31630) is a mouse monoclonal antibody and is validated for use in Flow Cyt (Intra) in rat samples.
Anti-CD68 antibody [ED1] (ab31630) specifically detects CD68 (UniProt ID: P34810; Molecular weight: 35kDa) and is sold in 125 µg selling sizes.

Quality and Validation

Abcam's high quality validation processes ensure Anti-CD68 antibody [ED1] (ab31630) has high sensitivity and specificity.
Anti-CD68 antibody [ED1] (ab31630) has been cited over 403 times in peer reviewed journals and is trusted by the scientific community.
Anti-CD68 antibody [ED1] (ab31630) has 20 independent reviews from customers.

Target Information

CD68 is a transmembrane glycoprotein predominantly expressed on macrophages, playing a crucial role in phagocytosis and antigen presentation. It is involved in various pathological conditions, including neuroinflammatory diseases, where it helps clear cellular debris and modulate immune response. CD68 expression is associated with several diseases, particularly those involving macrophage activity, including, cancer, neuroinflammation, Granular cell tumor and histiocytic sarcoma.

This product should be stored undiluted.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from tissue culture supernatant.
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.
Biological function summary

CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.

Pathways

CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.

CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Cd68
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Publications (490)

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Alena Kisel,Minrui Luo,Matthew D Bailey,Harman Ghuman,Matthew Rotz,Vinícius P Campos,Marcelo A C Vieira,T Kevin Hitchens,Thomas J Meade,Michel Modo

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Andreea Iuliana Cojocaru,Kaouthar Kefi,Jean-Daniel Masson,Laurent Tiret,Frederic Relaix,Valentina Taglietti

Journal of molecular histology 56:106 PubMed40080193

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Exogenous mitochondria added on benefits for cellular prion protein overexpression in adipose-derived mesenchymal stem cells treatment on intracranial hemorrhage rat.

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Kun-Chen Lin,Jui-Ning Yeh,Pei-Hsun Sung,Tsung-Cheng Yin,John Y Chiang,Chi-Ruei Huang,Yi-Ling Chen,Yi-Ting Wang,Kuan-Hung Chen,Hon-Kan Yip

International journal of nanomedicine 20:2761-2778 PubMed40061881

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Quercetin-Loaded Nanoparticle-Modified Decellularized Tissue-Engineered Vascular Graft Regulates Macrophage Polarization and Promotes In Vivo Graft Remodeling.

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Tun Wang,Zhenyu He,Peng Lu,Sheng Liao,Siyuan Cheng,Tianjian Wang,Yangyang An,Zibo Cheng,Chang Shu

Nutrients 17: PubMed40005027

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The Effects of White Wine and Ethanol Consumption on the Proliferative Phase of Repair After a Surgically Induced Myocardial Infarction in Rats.

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Danica Boban,Ivica Grković,Ana Marija Dželalija,Diana Gujinović,Ivana Mudnić,Mladen Boban

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Inhibition of kinin B1 receptor alleviates SARS-CoV-2-induced long-lasting cardiovascular complications.

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Drew Theobald,Lisandra E de Castro Braz,Shaw M Akula,Jeffrey B Eells,Srinivas Sriramula

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Xuewei Bi,Zhinan Mao,Linhao Li,Yilin Zhang,Lingbing Yang,Sen Hou,Juan Guan,Yufeng Zheng,Xiaoming Li,Yubo Fan
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