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Rabbit Recombinant Monoclonal CD68 antibody. Carrier free. Suitable for WB, ICC/IF, mIHC, IHC-P and reacts with Human samples. Cited in 1 publication.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - BSA and Azide free (AB227458), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [EPR20545] - BSA and Azide free (AB227458), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - BSA and Azide free (AB227458), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [EPR20545] - BSA and Azide free (AB227458), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - BSA and Azide free (AB227458), expandable thumbnail

Publications

  • Frontiers in immunology 14:11626692023
    Naproxen chemoprevention induces proliferation of cytotoxic lymphocytes in Lynch Syndrome colorectal mucosa.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Charles M Bowen,Nan Deng,Laura Reyes-Uribe,Edwin Roger Parra,Pedro Rocha,Luisa M Solis,Ignacio I Wistuba,Valerie O Sepeda,Lana Vornik,Marjorie Perloff,Eva Szabo,Asad Umar,Krishna M Sinha,Powel H Brown,Eduardo Vilar
    PubMed 37207208

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFmIHCIHC-P
Human
Expected
Tested
Tested
Tested

Expected
Expected

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Target data

Function

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

Alternative names

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Rabbit Recombinant Monoclonal CD68 antibody. Carrier free. Suitable for WB, ICC/IF, mIHC, IHC-P and reacts with Human samples. Cited in 1 publication.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR20545

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab227458 is the carrier-free version of Anti-CD68 antibody [EPR20545] ab213363.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

Activity summary

CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.

Biological function summary

CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.

Pathways

CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.

Associated diseases and disorders

CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

    Clone EPR20545 (ab227458) has been successfully conjugated by Abcam. This image was generated using Anti-CD68 antibody [EPR20545] (Alexa Fluor® 647). Please refer to Alexa Fluor® 647 Anti-CD68 antibody [EPR20545] ab224029 for protocol details.

    IHC image of CD68 staining in a section of formalin-fixed paraffin-embedded human prefrontal cortex tissue.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6) in a Biocare Medical NxGen pressure cooker using retrieval settings of 110oC for 20 minutes. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature.  The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with Alexa Fluor® 647 Anti-CD68 antibody [EPR20545] ab224029 at 1/1000 dilution (shown in red) and counterstained using Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

    Immunofluorescent analysis of 100% methanol-fixed THP-1 (human monocytic leukemia cell line) cells labeling CD68 with Anti-CD68 antibody [EPR20545] ab213363 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on THP-1 cells.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD68 antibody [EPR20545] ab213363).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue, labeling CD68 with Anti-CD68 antibody [EPR20545] ab213363 at 1/8000 dilution, followed by Goat anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on macrophages of human tonsil is observed (PMID: 19543531). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD68 antibody [EPR20545] ab213363).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

    Immunofluorescent analysis of 100% methanol-fixed U937 (human histiocytic lymphoma cell line) cells labeling CD68 with Anti-CD68 antibody [EPR20545] ab213363 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on U937 cells.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD68 antibody [EPR20545] ab213363).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

    This IHC data was generated using the same anti-CD68 antibody clone, EPR20545, in a different buffer formulation (cat# Anti-CD68 antibody [EPR20545] ab213363).

    Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue labeling CD68 with Anti-CD68 antibody [EPR20545] ab213363 at 1/8000 dilution, followed by Goat anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on macrophages of human cervical carcinoma is observed (PMID: 12118106). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD68 with Anti-CD68 antibody [EPR20545] ab213363 at 1/8000 dilution, followed by Goat anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on Kupffer cells of human liver is observed (PMID: 12118106). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD68 antibody [EPR20545] ab213363).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

    Tissue Microarrays stained for "Anti-CD68 antibody [EPR20545]" using "Anti-CD68 antibody [EPR20545] ab213363"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). The sections were incubated with Anti-CD68 antibody [EPR20545] ab213363 at +4°C overnight followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

  • Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

    CD68 immunohistochemistry staining of human colon using rabbit anti-CD68 antibody

    Fluorescence multiplex immunohistochemical analysis of the human colon (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-liver FABP (Anti-liver FABP antibody [EPR20464] - BSA and Azide free ab240401 gray; Opal™690), anti-CD3 epsilon (Anti-CD3 epsilon antibody [SP7] ab16669 green; Opal™520) and anti-CD68 (Anti-CD68 antibody [EPR20545] ab213363 red; Opal™570) on human colon. Panel B: anti-liver FABP stained on enterocytes. Panel C: anti-CD3 epsilon stained on T cells. Panel D: anti-CD68 stained on macrophages. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of Anti-liver FABP antibody [EPR20464] - BSA and Azide free ab240401 (1/8000 dilution), Anti-CD3 epsilon antibody [SP7] ab16669 (1/150 dilution) and Anti-CD68 antibody [EPR20545] ab213363 (1/500 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD68 antibody [EPR20545] ab213363).

  • Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

    CD68 immunohistochemistry staining of human duodenum using rabbit anti-CD68 antibody

    Fluorescence multiplex immunohistochemical analysis of the human duodenum (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-liver FABP (Anti-liver FABP antibody [EPR20464] - BSA and Azide free ab240401 gray; Opal™690), anti-CD3 epsilon (Anti-CD3 epsilon antibody [SP7] ab16669 green; Opal™520) and anti-CD68 (Anti-CD68 antibody [EPR20545] ab213363 red; Opal™570) on human duodenum. Panel B: anti-liver FABP stained on enterocytes. Panel C: anti-CD3 epsilon stained on T cells. Panel D: anti-CD68 stained on macrophages. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of Anti-liver FABP antibody [EPR20464] - BSA and Azide free ab240401 (1/8000 dilution), Anti-CD3 epsilon antibody [SP7] ab16669 (1/150 dilution) and Anti-CD68 antibody [EPR20545] ab213363 (1/500 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD68 antibody [EPR20545] ab213363).

  • Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

    This data was developed using Anti-CD68 antibody [EPR20545] ab213363, the same antibody clone in a different buffer formulation.

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human tonsil tissue staining PD-L1 with Anti-PD-L1 antibody [28-8] ab205921 at a 1:500 (2.19 ug/ml) dilution; PD1 with Anti-PD1 antibody [CAL20] ab237728 at 1:2000 (0.525 ug/ml) dilution and CD68 with Anti-CD68 antibody [EPR20545] ab213363 at 1:500 (1.26 ug/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

    Panel A: merged staining of anti-CD68 (magenta; Opal690), anti-PD-L1 (green; Opal520) and anti-PD1 (red; Opal570) on human tonsil.

    Panel B: anti-PD-L1 stained on cells involved in T cell inhibition.

    Panel C: anti-PD1 stained on antigen-stimulated T cells.

    Panel D: anti-CD68 stained on macrophages.

    The section was incubated in three rounds of staining: in the order of Anti-CD68 antibody [EPR20545] ab213363 and Anti-PD-L1 antibody [28-8] ab205921 for 30 mins, then Anti-PD1 antibody [CAL20] ab237728 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

    This data was developed using Anti-CD68 antibody [EPR20545] ab213363, the same antibody clone in a different buffer formulation.

    Panel A: merged staining of anti-CD68 (gray; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-CD19 (red; Opal™570) on Formalin/PFA-fixed paraffin-embedded sections of human tonsil. Secondary antibody was Opal Polymer HRP Ms + Rb, and counterstaining was with DAPI.

    Panel B: anti-CD3 epsilon stained on T cells with Anti-CD3 epsilon antibody [SP7] ab16669 at 1/500 dilution
    Panel C: anti-CD19 stained on B cells with Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/5000 dilution
    Panel D: anti-CD68 stained on macrophages with Anti-CD68 antibody [EPR20545] ab213363 1/500 dilution

    The section was incubated in three rounds of staining: in the order of Anti-CD68 antibody [EPR20545] ab213363 and Anti-CD3 epsilon antibody [SP7] ab16669 for 30 mins, then Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

    This data was developed using Anti-CD68 antibody [EPR20545] ab213363, the same antibody clone in a different buffer formulation.
    Panel A: merged staining of anti-CD68 (magenta; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-CD19 (red; Opal™570) on Formalin/PFA-fixed paraffin-embedded sections of human spleen. Secondary antibody was Opal Polymer HRP Ms + Rb, and couterstaining was with DAPI.
    Panel B: anti-CD3 epsilon stained on T cells with Anti-CD3 epsilon antibody [SP7] ab16669 at 1/500 dilution
    Panel C: anti-CD19 stained on B cells with Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/5000 dilution
    Panel D: anti-CD68 stained on macrophages with Anti-CD68 antibody [EPR20545] ab213363 1/500 dilution
    The section was incubated in three rounds of staining: in the order of Anti-CD68 antibody [EPR20545] ab213363 and Anti-CD3 epsilon antibody [SP7] ab16669 for 30 mins, then Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

    CD68 immunohistochemistry staining of human thymus using rabbit anti-CD68 antibody

    This data was developed using Anti-CD68 antibody [EPR20545] ab213363, the same antibody clone in a different buffer formulation.


    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human thymus tissue labeling CD3 epsilon with Anti-CD3 epsilon antibody [SP7] ab16669 at 1/500 dilution, LY75/DEC-205 with Anti-LY75/DEC-205 antibody [EPR5233] - BSA and Azide free ab208649 at 1/15000, and CD68 with Anti-CD68 antibody [EPR20545] ab213363 at 1/500 dilution.

    Panel A: merged staining of anti-CD68 (magenta; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-LY75/DEC-205 (red; Opal™570) on human thymus.
    Panel B: anti-CD3 epsilon stained on T cells.
    Panel C: anti-LY75/DEC-205 stained on thymic cortical epithelium and dendritic cells.
    Panel D: anti-CD68 stained on macrophages.

    Sections  were treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins before antibody incubation. The section was incubated in three rounds of staining: in the order of Anti-CD68 antibody [EPR20545] ab213363, Anti-CD3 epsilon antibody [SP7] ab16669, and Anti-LY75/DEC-205 antibody [EPR5233] - BSA and Azide free ab208649 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    DAPI was used as a nuclear counterstain.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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