Anti-CD68 antibody [EPR20545] - BSA and Azide free

13 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

  Clone EPR20545 (ab227458) has been successfully conjugated by Abcam. This image was generated using Anti-CD68 antibody [EPR20545] (Alexa Fluor® 647). Please refer to Alexa Fluor® 647 Anti-CD68 antibody [EPR20545] ab224029 for protocol details.

  IHC image of CD68 staining in a section of formalin-fixed paraffin-embedded human prefrontal cortex tissue.

  The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6) in a Biocare Medical NxGen pressure cooker using retrieval settings of 110^oC for 20 minutes. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature.  The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with Alexa Fluor® 647 Anti-CD68 antibody [EPR20545] ab224029 at 1/1000 dilution (shown in red) and counterstained using Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

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  Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

  Immunofluorescent analysis of 100% methanol-fixed THP-1 (human monocytic leukemia cell line) cells labeling CD68 with Anti-CD68 antibody [EPR20545] ab213363 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on THP-1 cells.

  The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD68 antibody [EPR20545] ab213363).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue, labeling CD68 with Anti-CD68 antibody [EPR20545] ab213363 at 1/8000 dilution, followed by Goat anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on macrophages of human tonsil is observed (PMID: 19543531). Counter stained with hematoxylin.
  

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-Rabbit IgG H&L (HRP) Ready to use.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD68 antibody [EPR20545] ab213363).

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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  Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

  Immunofluorescent analysis of 100% methanol-fixed U937 (human histiocytic lymphoma cell line) cells labeling CD68 with Anti-CD68 antibody [EPR20545] ab213363 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on U937 cells.

  The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD68 antibody [EPR20545] ab213363).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

  This IHC data was generated using the same anti-CD68 antibody clone, EPR20545, in a different buffer formulation (cat# Anti-CD68 antibody [EPR20545] ab213363).
  

  Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue labeling CD68 with Anti-CD68 antibody [EPR20545] ab213363 at 1/8000 dilution, followed by Goat anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on macrophages of human cervical carcinoma is observed (PMID: 12118106). Counter stained with hematoxylin.

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-Rabbit IgG H&L (HRP) Ready to use.

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

  Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD68 with Anti-CD68 antibody [EPR20545] ab213363 at 1/8000 dilution, followed by Goat anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on Kupffer cells of human liver is observed (PMID: 12118106). Counter stained with hematoxylin.
  

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-Rabbit IgG H&L (HRP) Ready to use.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD68 antibody [EPR20545] ab213363).

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

  Tissue Microarrays stained for "Anti-CD68 antibody [EPR20545]" using "Anti-CD68 antibody [EPR20545] ab213363"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). The sections were incubated with Anti-CD68 antibody [EPR20545] ab213363 at +4°C overnight followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

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  Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

  CD68 immunohistochemistry staining of human colon using rabbit anti-CD68 antibody
  
  

  Fluorescence multiplex immunohistochemical analysis of the human colon (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-liver FABP (Anti-liver FABP antibody [EPR20464] - BSA and Azide free ab240401 gray; Opal™690), anti-CD3 epsilon (Anti-CD3 epsilon antibody [SP7] ab16669 green; Opal™520) and anti-CD68 (Anti-CD68 antibody [EPR20545] ab213363 red; Opal™570) on human colon. Panel B: anti-liver FABP stained on enterocytes. Panel C: anti-CD3 epsilon stained on T cells. Panel D: anti-CD68 stained on macrophages. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of Anti-liver FABP antibody [EPR20464] - BSA and Azide free ab240401 (1/8000 dilution), Anti-CD3 epsilon antibody [SP7] ab16669 (1/150 dilution) and Anti-CD68 antibody [EPR20545] ab213363 (1/500 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD68 antibody [EPR20545] ab213363).

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  Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

  CD68 immunohistochemistry staining of human duodenum using rabbit anti-CD68 antibody
  
  

  Fluorescence multiplex immunohistochemical analysis of the human duodenum (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-liver FABP (Anti-liver FABP antibody [EPR20464] - BSA and Azide free ab240401 gray; Opal™690), anti-CD3 epsilon (Anti-CD3 epsilon antibody [SP7] ab16669 green; Opal™520) and anti-CD68 (Anti-CD68 antibody [EPR20545] ab213363 red; Opal™570) on human duodenum. Panel B: anti-liver FABP stained on enterocytes. Panel C: anti-CD3 epsilon stained on T cells. Panel D: anti-CD68 stained on macrophages. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of Anti-liver FABP antibody [EPR20464] - BSA and Azide free ab240401 (1/8000 dilution), Anti-CD3 epsilon antibody [SP7] ab16669 (1/150 dilution) and Anti-CD68 antibody [EPR20545] ab213363 (1/500 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD68 antibody [EPR20545] ab213363).

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  Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

  This data was developed using Anti-CD68 antibody [EPR20545] ab213363, the same antibody clone in a different buffer formulation.

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human tonsil tissue staining PD-L1 with Anti-PD-L1 antibody [28-8] ab205921 at a 1:500 (2.19 ug/ml) dilution; PD1 with Anti-PD1 antibody [CAL20] ab237728 at 1:2000 (0.525 ug/ml) dilution and CD68 with Anti-CD68 antibody [EPR20545] ab213363 at 1:500 (1.26 ug/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

  Panel A: merged staining of anti-CD68 (magenta; Opal^™690), anti-PD-L1 (green; Opal^™520) and anti-PD1 (red; Opal^™570) on human tonsil.
  
  Panel B: anti-PD-L1 stained on cells involved in T cell inhibition.
  
  Panel C: anti-PD1 stained on antigen-stimulated T cells.
  
  Panel D: anti-CD68 stained on macrophages.

  The section was incubated in three rounds of staining: in the order of Anti-CD68 antibody [EPR20545] ab213363 and Anti-PD-L1 antibody [28-8] ab205921 for 30 mins, then Anti-PD1 antibody [CAL20] ab237728 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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  Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

  This data was developed using Anti-CD68 antibody [EPR20545] ab213363, the same antibody clone in a different buffer formulation.
  
  Panel A: merged staining of anti-CD68 (gray; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-CD19 (red; Opal™570) on Formalin/PFA-fixed paraffin-embedded sections of human tonsil. Secondary antibody was Opal Polymer HRP Ms + Rb, and counterstaining was with DAPI.
  
  Panel B: anti-CD3 epsilon stained on T cells with Anti-CD3 epsilon antibody [SP7] ab16669 at 1/500 dilution
  Panel C: anti-CD19 stained on B cells with Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/5000 dilution
  Panel D: anti-CD68 stained on macrophages with Anti-CD68 antibody [EPR20545] ab213363 1/500 dilution
  
  The section was incubated in three rounds of staining: in the order of Anti-CD68 antibody [EPR20545] ab213363 and Anti-CD3 epsilon antibody [SP7] ab16669 for 30 mins, then Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

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  Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

  This data was developed using Anti-CD68 antibody [EPR20545] ab213363, the same antibody clone in a different buffer formulation.
  Panel A: merged staining of anti-CD68 (magenta; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-CD19 (red; Opal™570) on Formalin/PFA-fixed paraffin-embedded sections of human spleen. Secondary antibody was Opal Polymer HRP Ms + Rb, and couterstaining was with DAPI.
  Panel B: anti-CD3 epsilon stained on T cells with Anti-CD3 epsilon antibody [SP7] ab16669 at 1/500 dilution
  Panel C: anti-CD19 stained on B cells with Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/5000 dilution
  Panel D: anti-CD68 stained on macrophages with Anti-CD68 antibody [EPR20545] ab213363 1/500 dilution
  The section was incubated in three rounds of staining: in the order of Anti-CD68 antibody [EPR20545] ab213363 and Anti-CD3 epsilon antibody [SP7] ab16669 for 30 mins, then Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

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  Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)

  CD68 immunohistochemistry staining of human thymus using rabbit anti-CD68 antibody
  
  This data was developed using Anti-CD68 antibody [EPR20545] ab213363, the same antibody clone in a different buffer formulation.
  
  
  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human thymus tissue labeling CD3 epsilon with Anti-CD3 epsilon antibody [SP7] ab16669 at 1/500 dilution, LY75/DEC-205 with Anti-LY75/DEC-205 antibody [EPR5233] - BSA and Azide free ab208649 at 1/15000, and CD68 with Anti-CD68 antibody [EPR20545] ab213363 at 1/500 dilution.
  
  Panel A: merged staining of anti-CD68 (magenta; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-LY75/DEC-205 (red; Opal™570) on human thymus.
  Panel B: anti-CD3 epsilon stained on T cells.
  Panel C: anti-LY75/DEC-205 stained on thymic cortical epithelium and dendritic cells.
  Panel D: anti-CD68 stained on macrophages.
  
  Sections  were treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins before antibody incubation. The section was incubated in three rounds of staining: in the order of Anti-CD68 antibody [EPR20545] ab213363, Anti-CD3 epsilon antibody [SP7] ab16669, and Anti-LY75/DEC-205 antibody [EPR5233] - BSA and Azide free ab208649 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
  
  DAPI was used as a nuclear counterstain.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.