Goat Recombinant Monoclonal CD68 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - Chimeric – BSA and Azide free (AB289677), expandable thumbnail](https://content.abcam.com/products/images/cd68-antibody-epr20545-goat-igg-chimeric-bsa-and-azide-free-ab289677--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img164712.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - Chimeric – BSA and Azide free (AB289677)")
![Western blot - Anti-CD68 antibody [EPR20545] - Chimeric – BSA and Azide free (AB289677), expandable thumbnail](https://content.abcam.com/products/images/cd68-antibody-epr20545-goat-igg-chimeric-bsa-and-azide-free-ab289677--western-blot-img171346.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD68 antibody [EPR20545] - Chimeric – BSA and Azide free (AB289677)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - Chimeric – BSA and Azide free (AB289677), expandable thumbnail](https://content.abcam.com/products/images/cd68-antibody-epr20545-goat-igg-chimeric-bsa-and-azide-free-ab289677--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img178049.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - Chimeric – BSA and Azide free (AB289677)")
Key facts
- Isotype
IgG
- Host species
Goat
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Alternative names
Macrosialin, Gp110, CD68
Recommended products
Goat Recombinant Monoclonal CD68 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Alternative names
Macrosialin, Gp110, CD68
Key facts
- Isotype
IgG
- Host species
Goat
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR20545
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab289677 is the carrier-free version of Anti-CD68 antibody [EPR20545] - Goat IgG (Chimeric) ab289671.
This goat monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-CD68 antibody [EPR20545] ab213363). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.
- Biological function summary
CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.
- Pathways
CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.
- Associated diseases and disorders
CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - Chimeric – BSA and Azide free (ab289677), expandable thumbnail](https://content.abcam.com/products/images/cd68-antibody-epr20545-goat-igg-chimeric-bsa-and-azide-free-ab289677--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img164712.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - Chimeric – BSA and Azide free (ab289677)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - Chimeric – BSA and Azide free (ab289677)
This data was developed using Anti-CD68 antibody [EPR20545] - Goat IgG (Chimeric) ab289671 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD68 with Anti-CD68 antibody [EPR20545] - Goat IgG (Chimeric) ab289671 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the macrophages in human tonsil. The section was incubated with Anti-CD68 antibody [EPR20545] - Goat IgG (Chimeric) ab289671 for 30 mins at room temperature and followed by goat specific IgG antibody (Rabbit Anti-Goat IgG H&L preadsorbed ab97101) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Western blot - Anti-CD68 antibody [EPR20545] - Chimeric – BSA and Azide free (ab289677), expandable thumbnail](https://content.abcam.com/products/images/cd68-antibody-epr20545-goat-igg-chimeric-bsa-and-azide-free-ab289677--western-blot-img171346.jpg "Western blot - Anti-CD68 antibody [EPR20545] - Chimeric – BSA and Azide free (ab289677)")
Western blot - Anti-CD68 antibody [EPR20545] - Chimeric – BSA and Azide free (ab289677)
This data was developed using Anti-CD68 antibody [EPR20545] - Goat IgG (Chimeric) ab289671 the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times - Lanes 1-2: 3 minutes; Lane 3: 37 seconds.
The observed molecular weight is consistent with the literature (PMID:18405323, 11739566, 16710801).
All lanes: Western blot - Anti-CD68 antibody [EPR20545] - Goat IgG (Anti-CD68 antibody [EPR20545] - Goat IgG (Chimeric) ab289671) at 1/1000 dilution
Lane 1: Human tonsil tissue lysate at 20 µg
Lane 2: Human liver tissue lysate at 20 µg
Lane 3: THP-1 (human monocytic leukemia monocyte cell line) whole cell lysate at 20 µg
SecondaryAll lanes: Rabbit anti-Goat IgG, (H+L), Peroxidase conjugated at 1/5000 dilution
Predicted band size: 37 kDa
Observed band size: 110 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - Chimeric – BSA and Azide free (ab289677), expandable thumbnail](https://content.abcam.com/products/images/cd68-antibody-epr20545-goat-igg-chimeric-bsa-and-azide-free-ab289677--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img178049.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - Chimeric – BSA and Azide free (ab289677)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - Chimeric – BSA and Azide free (ab289677)
This data was developed using Anti-CD68 antibody [EPR20545] - Goat IgG (Chimeric) ab289671 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling CD68 with Anti-CD68 antibody [EPR20545] - Goat IgG (Chimeric) ab289671 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the Kuffer cells in human liver. The section was incubated with Anti-CD68 antibody [EPR20545] - Goat IgG (Chimeric) ab289671 for 30 mins at room temperature and followed by goat specific IgG antibody (Rabbit Anti-Goat IgG H&L preadsorbed ab97101) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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