Anti-CD68 antibody [FA-11] is a rat recombinant monoclonal antibody that is used to detect CD68 in Flow cytometry (Intra), ICC/IF. Suitable for Mouse samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Cited in over 310 publications
- Top cited clone for CD68 on the market
Images
![Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [FA-11] (AB53444), expandable thumbnail](https://content.abcam.com/products/images/cd68-antibody-fa-11-ab53444--immunocytochemistry-immunofluorescence-img408366.png/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [FA-11] (AB53444)")
![Flow Cytometry (Intracellular) - Anti-CD68 antibody [FA-11] (AB53444), expandable thumbnail](https://content.abcam.com/products/images/cd68-antibody-fa-11-ab53444--flow-cytometry-intracellular-img408367.png/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CD68 antibody [FA-11] (AB53444)")
Publications
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- Journal of neuroinflammation 20:2822023Inhibition of microfold cells ameliorates early pathological phenotypes by modulating microglial functions in Alzheimer's disease mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNamkwon Kim,In Gyoung Ju,Seung Ho Jeon,Yeongae Lee,Min-Ji Jung,Min Sung Gee,Jae Seok Cho,Kyung-Soo Inn,Lee Ann Garrett-Sinha,Myung Sook Oh,Jong Kil LeePubMed 38012646
- Scientific reports 13:188952023Altered peripheral taste function in a mouse model of inflammatory bowel disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuangkuo Dong,Khaylie Boothe,Lianying He,Yang Shi,Lynnette Phillips McCluskeyPubMed 37919307
- Molecular neurobiology 61:2569-25892023Integrated Analysis of Chromatin and Transcriptomic Profiling Identifies PU.1 as a Core Regulatory Factor in Microglial Activation Induced by Chronic Cerebral Hypoperfusion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZengyu Zhang,Pengpeng Jin,Zimin Guo,Zhilan Tu,Hualan Yang,Mengting Hu,Qinghua Li,Xingdang Liu,Weiwei Li,Shuangxing HouPubMed 37917300
- Biomedicines 11:2023HER-2 Expression in Colorectal Cancer and Its Correlation with Immune Cell Infiltration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDi Yang,Bo Wang,Yinuo Li,Jingyao Zhang,Xuantong Gong,Hao Qin,Yan Wang,Yahui Zhao,Yong WangPubMed 38001890
- Frontiers in neuroscience 17:12764952023Regulation of microglial responses after pediatric traumatic brain injury: exploring the role of SHIP-1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErskine Chu,Richelle Mychasiuk,Tabitha R F Green,Akram Zamani,Larissa K Dill,Rishabh Sharma,April L Raftery,Evelyn Tsantikos,Margaret L Hibbs,Bridgette D SemplePubMed 37901420
- Nature communications 14:62942023A human antibody against pathologic IAPP aggregates protects beta cells in type 2 diabetes models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFabian Wirth,Fabrice D Heitz,Christine Seeger,Ioana Combaluzier,Karin Breu,Heather C Denroche,Julien Thevenet,Melania Osto,Paolo Arosio,Julie Kerr-Conte,C Bruce Verchere,François Pattou,Thomas A Lutz,Marc Y Donath,Christoph Hock,Roger M Nitsch,Jan GrimmPubMed 37813862
- Neuromolecular medicine 25:616-6312023CD137L Inhibition Ameliorates Hippocampal Neuroinflammation and Behavioral Deficits in a Mouse Model of Sepsis-Associated Encephalopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFang Qiu,Yueming Liu,Yang Liu,Zhuyun Zhao,Lile Zhou,Pengfei Chen,Yunbo Du,Yanmei Wang,Huimin Sun,Changchun Zeng,Xiaokang Wang,Yuqiang Liu,Haobo Pan,Changneng KePubMed 37796401
- International journal of nanomedicine 18:5495-55102023Intranasal Delivery of Endothelial Cell-Derived Extracellular Vesicles with Supramolecular Gel Attenuates Myocardial Ischemia-Reperfusion Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunzhuo Wang,Ying Tan,Yang Dai,Ke Hu,Xi Tan,Shaoli Jiang,Guannan Li,Xinlin Zhang,Lina Kang,Xiaojian Wang,Biao XuPubMed 37791323
- International journal of molecular medicine 52:2023Adropin attenuates pancreatitis‑associated lung injury through PPARγ phosphorylation‑related macrophage polarization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFadian Ding,Guozhong Liu,Feng Gao,Zhou Zheng,Yupu Hong,Youting Chen,Shangeng WengPubMed 37654184
- Life science alliance 6:2023Inhibition of protein arginine deiminase 4 prevents inflammation-mediated heart failure in arthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLukas A Heger,Nicolas Schommer,Shoichi Fukui,Stijn Van Bruggen,Casey E Sheehy,Long Chu,Sridharan Rajagopal,Dhanalakshmi Sivanandhan,Bruce Ewenstein,Denisa D WagnerPubMed 37500179
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Flow Cyt (Intra) | IHC-Fr | |
|---|---|---|---|
| Mouse | Tested | Tested | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes Membrane permeabilisation is required for this application. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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Target data
Function
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Alternative names
CD68, Macrosialin, Cd68
Recommended products
Anti-CD68 antibody [FA-11] is a rat recombinant monoclonal antibody that is used to detect CD68 in Flow cytometry (Intra), ICC/IF. Suitable for Mouse samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Cited in over 310 publications
- Top cited clone for CD68 on the market
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- FA-11
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Anti-CD68 antibody [FA-11] (ab53444) is a rat recombinant monoclonal antibody and is validated for use in Flow Cyt (Intra), ICC/IF in dog, human, mouse, rat samples.
Anti-CD68 antibody [FA-11] (ab53444) has been cited over 312 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-CD68 antibody [FA-11] (ab53444) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-CD68 antibody [FA-11] (ab53444) has 24 independent reviews from customers.
Anti-CD68 antibody [FA-11] (Anti-Macrosialin antibody) (ab53444) specifically detects CD68 (UniProt ID: P34810; Molecular weight: 35kDa) and is sold in 100 µg and 1 mg selling sizes.
Conjugation-ready, carrier free format available for antibody clone FA-11 - Anti-CD68 antibody [FA-11] - BSA and Azide free ab237968.
Antibody clone FA-11 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 405, Alexa Fluor® 488, Alexa Fluor® 647, HRP, PE, PerCP, APC (Alexa Fluor® 405 Anti-CD68 antibody [FA-11] ab199571, Alexa Fluor® 488 Anti-CD68 antibody [FA-11] ab201844, Alexa Fluor® 647 Anti-CD68 antibody [FA-11] ab201845, HRP Anti-CD68 antibody [FA-11] ab216526, PE Anti-CD68 antibody [FA-11] ab216701, PerCP Anti-CD68 antibody [FA-11] ab220509, APC Anti-CD68 antibody [FA-11] ab221251).
CD68 is a transmembrane glycoprotein predominantly expressed on macrophages, playing a crucial role in the immune system. It is involved in phagocytosis and antigen presentation, making it essential for immune responses. CD68 is expressed in various macrophage subtypes, including M1 (pro-inflammatory) and M2 (anti-inflammatory), contributing to both inflammation and tissue repair. In the central nervous system, CD68 is a marker for microglia, the brain's immune cells, and is involved in clearing cellular debris and modulating inflammatory responses. Its expression levels can influence the progression of neurodegenerative diseases and other immunological conditions.
Although some customers have had success with this antibody in IHC-P, we are unable to obtain positive results in this application and so cannot recommend it for IHC-P. We batch test the antibody in IHC-Fr.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.
- Biological function summary
CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.
- Pathways
CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.
- Associated diseases and disorders
CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [FA-11] (ab53444), expandable thumbnail](https://content.abcam.com/products/images/cd68-antibody-fa-11-ab53444--immunocytochemistry-immunofluorescence-img408366.png "Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [FA-11] (ab53444)")
Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [FA-11] (ab53444)
CD68 Immunocytochemistry/ Immunofluorescence staining using rat Anti-CD68 antibody
RAW 264.7 cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab53444 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Rat secondary antibody (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157) at 1/1000 dilution at RT for 45 min. Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab53444 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI. Confocal image showing cytoplasmic staining in RAW 264.7 cell line.
![Flow Cytometry (Intracellular) - Anti-CD68 antibody [FA-11] (ab53444), expandable thumbnail](https://content.abcam.com/products/images/cd68-antibody-fa-11-ab53444--flow-cytometry-intracellular-img408367.png "Flow Cytometry (Intracellular) - Anti-CD68 antibody [FA-11] (ab53444)")
Flow Cytometry (Intracellular) - Anti-CD68 antibody [FA-11] (ab53444)
Flow Cytometry analysis of 4% paraformaldehyde-fixed RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling CD68 with ab53444 at a 1/100 dilution (1 μg) (Red) compared to Rat monoclonal IgG - Isotype Control (Black) and cells without incubation with primary antibody and secondary antibody (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed ab150161) at 1/2000 dilution was used as the secondary antibody.
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