AB53444

Anti-CD68 antibody [FA-11]

Name: Anti-CD68/macrosialin antibody [FA-11] - macrophage marker (ab53444) | Abcam
Brand: Abcam Limited
SKU: AB53444
Price: 250 USD
Availability: InStock
Rating: 4 (25 reviews)

Lab Essentials
What is this?

(25 Reviews)

(416 Publications)

Anti-CD68 antibody [FA-11] (ab53444) is a rat monoclonal antibody detecting CD68 in Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Mouse.

- Over 310 publications
- Trusted since 2007

View Alternative Names

CD68, Macrosialin, Cd68

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [FA-11] (AB53444)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [FA-11] (AB53444)

RAW 264.7 cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab53444 at 1 : 50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Rat secondary antibody (ab150157) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab53444 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI. Confocal image showing cytoplasmic staining in RAW 264.7 cell line.

Flow Cytometry (Intracellular) - Anti-CD68 antibody [FA-11] (AB53444)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CD68 antibody [FA-11] (AB53444)

Flow Cytometry analysis of 4% paraformaldehyde-fixed RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling CD68 with ab53444 at a 1/100 dilution (1 μg) (Red) compared to Rat monoclonal IgG - Isotype Control (Black) and cells without incubation with primary antibody and secondary antibody (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.

Carrier free

Anti-CD68 antibody [FA-11] - BSA and Azide free
660 APC

APC Anti-CD68 antibody [FA-11]
675 PerCP

PerCP Anti-CD68 antibody [FA-11]
HRP

HRP Anti-CD68 antibody [FA-11]
578 PE

PE Anti-CD68 antibody [FA-11]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CD68 antibody [FA-11]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD68 antibody [FA-11]
421 Alexa Fluor® 405

Alexa Fluor® 405 Anti-CD68 antibody [FA-11]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

FA-11

Isotype

IgG2a

Carrier free

Reacts with

Mouse

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "Membrane permeabilisation is required for this application.<a href='/en-us/products/primary-antibodies/rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18450'>ab18450</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "0.1-5 µg/mL", "IHCFr-species-notes": "" } } }

style

left-column

Product details

Anti-CD68 antibody [FA-11] (ab53444) is a rat recombinant monoclonal antibody and is validated for use in Flow Cyt (Intra), ICC/IF in dog, human, mouse, rat samples.

Anti-CD68 antibody [FA-11] (ab53444) has been cited over 312 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality manufacturing and validation processes ensure Anti-CD68 antibody [FA-11] (ab53444) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-CD68 antibody [FA-11] (ab53444) has 24 independent reviews from customers.

Anti-CD68 antibody [FA-11] (Anti-Macrosialin antibody) (ab53444) specifically detects CD68 (UniProt ID: P34810; Molecular weight: 35kDa) and is sold in 100 µg and 1 mg selling sizes.

Conjugation-ready, carrier free format available for antibody clone FA-11 - ab237968.

Antibody clone FA-11 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 405, Alexa Fluor® 488, Alexa Fluor® 647, HRP, PE, PerCP, APC (ab199571, ab201844, ab201845, ab216526, ab216701, ab220509, ab221251).

CD68 is a transmembrane glycoprotein predominantly expressed on macrophages, playing a crucial role in the immune system. It is involved in phagocytosis and antigen presentation, making it essential for immune responses. CD68 is expressed in various macrophage subtypes, including M1 (pro-inflammatory) and M2 (anti-inflammatory), contributing to both inflammation and tissue repair. In the central nervous system, CD68 is a marker for microglia, the brain's immune cells, and is involved in clearing cellular debris and modulating inflammatory responses. Its expression levels can influence the progression of neurodegenerative diseases and other immunological conditions.

Although some customers have had success with this antibody in IHC-P, we are unable to obtain positive results in this application and so cannot recommend it for IHC-P. We batch test the antibody in IHC-Fr.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.

Biological function summary

CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.

Pathways

CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.

CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

See full target information Cd68

style

left-column

Publications (416)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:691 PubMed41053007

2025

Repeated ablations of mature Tmem10 oligodendrocytes recapitulates key pathological features of multiple sclerosis with prolonged demyelination.

Applications

Unspecified application

Species

Unspecified reactive species

Feiyan Zhu,Haijiao Huang,Yuting Shu,Guoru Ren,Bo Jing,Honglin Tan,Wanxiang Jiang,Yiyuan Cui,Paul F Worley,Bo Xiao,Mina Chen

Nature communications 16:8694 PubMed41027899

2025

Macrophage WDFY3 mitigates autoimmunity by enhancing efferocytosis and suppressing T cell activation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xun Wu,Ziyi Wang,Katherine R Croce,Fang Li,Jian Cui,Vivette D D'Agati,Rajesh K Soni,Ira Tabas,Ai Yamamoto,Hanrui Zhang

Acta neuropathologica communications 13:203 PubMed41024170

2025

Rab7a is required to degrade select blood-brain barrier junctional proteins after ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Azzurra Cottarelli,Danny Jamoul,Mary Claire Tuohy,Sanjid Shahriar,Michael Glendinning,Grace Prochilo,Aimee L Edinger,Ahmet Arac,Dritan Agalliu

Cell reports 44:116342 PubMed41004340

2025

Neuropeptide adrenomedullin remodels stemness and macrophage dynamics in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Heba Ali,Fatima Khan,Wenjing Xuan,Yang Liu,Yuyun Huang,Donovan Whitfield,Lizhi Pang,Peiwen Chen

Stem cell reviews and reports : PubMed40987919

2025

Investigating the Therapeutic Efficacy of Quality-Controlled, miR-146a-5p-Enriched Small Extracellular Vesicles Derived From MSCs Against Idiopathic Pulmonary Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Wang,Lingjiao Meng,Qiuhong Wang,Ruixue Rong,Yu Zhang,Xiaohui Zhao,Chen Liang,Huizhen Guo,Li Deng,Zengqi Tan,Feng Guan,Yi Tan

Journal of neuroinflammation 22:208 PubMed40877894

2025

P2X7-CaMKII drives 5-LOX nuclear translocation to impair microglial function after subarachnoid hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Peng-Fei Ding,Jia-Tong Zhang,Xiao-Long Zhu,Yue Cui,Chun-Lei Chen,Xun-Zhi Liu,Jun-Da Shen,Le-Xuan Zou,Yue Lu,Zong Zhuang,Chun-Hua Hang,Wei Li

PloS one 20:e0328001 PubMed40844997

2025

Ischemic duration determines extent of cardiac remodeling, and both early and delayed reperfusion prevent fatal cardiac rupture: Model comparison.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Zhao,Amanguli Ruze,Guo-Li Du,Min-Tao Gai,Jing Tang,Xiao-Ming Gao

Nature 646:452-461 PubMed40836081

2025

Axonal injury is a targetable driver of glioblastoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Melanie Clements,Wenhao Tang,Zan Florjanic Baronik,Holly Simpson Ragdale,Roger Oria,Dimitrios Volteras,Ian J White,Gordon Beattie,Imran Uddin,Tchern Lenn,Rachel Lindsay,Sara Castro Devesa,Saketh R Karamched,Mark F Lythgoe,Vahid Shahrezaei,Valerie M Weaver,Ryoichi Sugisawa,Federico Roncaroli,Samuel Marguerat,Ciaran S Hill,Simona Parrinello

Scientific reports 15:29741 PubMed40804251

2025

HSF1 in macrophages suppressed the progression of asthma via modulating SIRPα/SHP2-Dectin-1/ SYK mediated ROS and inflammatory responses.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Liu,Yingqian Zhang,Huifang Wu,Jingli Zhang,Yingxue Wang,Huifeng Zhang

Scientific reports 15:28976 PubMed40775512

2025

Identification and validation of hub genes related to neutrophil extracellular traps-mediated cell damage and immune recruitment during abdominal aortic aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanlong Lu,Heng Wang,Maolin Qiao,Runze Chang,Jinshan Chen,Lizheng Li,Keyi Fan,Sheng Yan,Ruijing Zhang,Honglin Dong

View all publications

style

left-column

style

left-column

style

right-column

Primary Antibodies

AB222914

Alexa Fluor® 488 Anti-CD68 antibody [KP1]

Recombinant|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD68 antibody [KP1] (AB222914)

Primary Antibodies

AB125157

Anti-CD68 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (AB125157)

Primary Antibodies

AB277938

Alexa Fluor® 594 Anti-CD68 antibody [EPR20545]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD68 antibody [EPR20545] (AB277938)

Complementary Products

Assay Kits

AB287167

Human CD68 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD68 ELISA Kit (AB287167)

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

Primary Antibodies

AB64693

Anti-Mannose Receptor antibody

BOND RX™ Validated

Western blot - Anti-Mannose Receptor antibody (AB64693)

Primary Antibodies

AB182422

Anti-CD163 antibody [EPR19518]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Flow Cytometry - Anti-CD163 antibody [EPR19518] (AB182422)

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD68 antibody [FA-11]

Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [FA-11] (AB53444)

Flow Cytometry (Intracellular) - Anti-CD68 antibody [FA-11] (AB53444)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (416)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise