AB237968

Anti-CD68 antibody [FA-11] - BSA and Azide free

Recombinant
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(10 Publications)

Rat Recombinant Monoclonal CD68 antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Mouse samples. Cited in 10 publications.

View Alternative Names

CD68, Macrosialin, Cd68

2 Images

Flow Cytometry (Intracellular) - Anti-CD68 antibody [FA-11] - BSA and Azide free (AB237968)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CD68 antibody [FA-11] - BSA and Azide free (AB237968)

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab53444). Flow Cytometry analysis of 4% paraformaldehyde-fixed RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling CD68 with ab53444 at a 1/100 dilution (1 μg) (Red) compared to Rat monoclonal IgG - Isotype Control (Black) and cells without incubation with primary antibody and secondary antibody (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [FA-11] - BSA and Azide free (AB237968)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [FA-11] - BSA and Azide free (AB237968)

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab53444). RAW 264.7 cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab53444 at 1 : 50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Rat secondary antibody (ab150157) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab53444 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI. Confocal image showing cytoplasmic staining in RAW 264.7 cell line.

Unconjugated

Anti-CD68 antibody [FA-11]
660 APC

APC Anti-CD68 antibody [FA-11]
675 PerCP

PerCP Anti-CD68 antibody [FA-11]
HRP

HRP Anti-CD68 antibody [FA-11]
578 PE

PE Anti-CD68 antibody [FA-11]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CD68 antibody [FA-11]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD68 antibody [FA-11]
421 Alexa Fluor® 405

Alexa Fluor® 405 Anti-CD68 antibody [FA-11]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

FA-11

Isotype

IgG2a

Carrier free

Yes

Reacts with

Mouse

Applications

ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "Membrane permeabilisation is required for this application.<a href='/en-us/products/primary-antibodies/rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18450'>ab18450</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "0.1-5 µg/mL", "IHCFr-species-notes": "" } } }

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Product details

ab23796 is the carrier-free version of ab53444.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.

Biological function summary

CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.

Pathways

CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.

CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

See full target information Cd68

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nature 644:790-798 PubMed40533564

2025

Kupffer cell programming by maternal obesity triggers fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Huang,Nora R Balzer,Lea Seep,Iva Splichalova,Nelli Blank-Stein,Maria Francesca Viola,Eliana Franco Taveras,Kerim Acil,Diana Fink,Franzisca Petrovic,Nikola Makdissi,Seyhmus Bayar,Katharina Mauel,Carolin Radwaniak,Jelena Zurkovic,Amir H Kayvanjoo,Klaus Wunderling,Malin Jessen,Mohamed H Yaghmour,Lukas Kenner,Thomas Ulas,Stephan Grein,Joachim L Schultze,Charlotte L Scott,Martin Guilliams,Zhaoyuan Liu,Florent Ginhoux,Marc D Beyer,Christoph Thiele,Felix Meissner,Jan Hasenauer,Dagmar Wachten,Elvira Mass

Developmental cell 59:869-881.e6 PubMed38359832

2024

Multimodal mass spectrometry imaging identifies cell-type-specific metabolic and lipidomic variation in the mammalian liver.

Applications

mIHC

Species

Mouse

Hua Tian,Presha Rajbhandari,Jay Tarolli,Aubrianna M Decker,Taruna V Neelakantan,Tina Angerer,Fereshteh Zandkarimi,Helen Remotti,Gilles Frache,Nicholas Winograd,Brent R Stockwell

JCI insight 9: PubMed37971881

2024

Efficient pulmonary lymphatic drainage is necessary for inflammation resolution in ARDS.

Applications

Unspecified application

Species

Unspecified reactive species

Pu-Hong Zhang,Wen-Wu Zhang,Shun-Shun Wang,Cheng-Hua Wu,Yang-Dong Ding,Xin-Yi Wu,Fang Gao Smith,Yu Hao,Sheng-Wei Jin

eLife 12: PubMed38127424

2023

Apoptosis recognition receptors regulate skin tissue repair in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Olivia Justynski,Kate Bridges,Will Krause,Maria Fernanda Forni,Quan M Phan,Teresa Sandoval-Schaefer,Kristyn Carter,Diane E King,Henry C Hsia,Michael I Gazes,Steven D Vyce,Ryan R Driskell,Kathryn Miller-Jensen,Valerie Horsley

Frontiers in neuroimaging 1:903531 PubMed37555169

2023

Synergistic photobiomodulation with 808-nm and 1064-nm lasers to reduce the β-amyloid neurotoxicity in the Alzheimer's disease models.

Applications

Unspecified application

Species

Unspecified reactive species

Renlong Zhang,Ting Zhou,Soham Samanta,Ziyi Luo,Shaowei Li,Hao Xu,Junle Qu

Mediators of inflammation 2023:4206316 PubMed36852396

2023

Induced Inflammatory and Oxidative Markers in Cerebral Microvasculature by Mentally Depressive Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yuequan Zhu,Yazeed Haddad,Ho Jun Yun,Xiaokun Geng,Yuchuan Ding

Alzheimer's research & therapy 15:33 PubMed36797783

2023

Early activation of Toll-like receptor-3 reduces the pathological progression of Alzheimer's disease in APP/PS1 mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Shang Wang,Taiyang Zhu,Wanyan Ni,Chao Zhou,Hui Zhou,Li Lin,Yuting Hu,Xiaoyu Sun,Jingjing Han,Yan Zhou,Guoliang Jin,Jie Zu,Hongjuan Shi,Xingxing Yang,Zuohui Zhang,Fang Hua

Frontiers in pharmacology 12:734589 PubMed34966274

2021

Tanyu Tongzhi Formula Delays Atherosclerotic Plaque Progression by Promoting Alternative Macrophage Activation PPARγ and AKT/ERK Signal Pathway in ApoE Knock-Out Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lan Ma,Xiaoce Dai,Chenxia Wu,Mingshuang Li,Hongzhuan Sheng,Wei Mao

Frontiers in immunology 12:766178 PubMed34721438

2021

TREM1 Regulates Neuroinflammatory Injury by Modulate Proinflammatory Subtype Transition of Microglia and Formation of Neutrophil Extracellular Traps Interaction With SYK in Experimental Subarachnoid Hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyan Wu,Hanhai Zeng,Chaoran Xu,Huaijun Chen,Linfeng Fan,Hang Zhou,Qian Yu,Xiongjie Fu,Yucong Peng,Feng Yan,Xiaobo Yu,Gao Chen

Bioscience reports 41: PubMed33969376

2021

Reduction of TMAO level enhances the stability of carotid atherosclerotic plaque through promoting macrophage M2 polarization and efferocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Weihao Shi,Yijun Huang,Zhou Yang,Liang Zhu,Bo Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD68 antibody [FA-11] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-CD68 antibody [FA-11] - BSA and Azide free (AB237968)

Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [FA-11] - BSA and Azide free (AB237968)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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