Anti-CD68 antibody [KP1]

7 product images

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  Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [KP1] (ab955)

  CD68 immunofluorescence staining of THP-1 cells using mouse anti-CD68 antibody
  
  Immunocytochemistry analysis of THP-1 (human monocytic leukemia monocyte) labelling CD68 with ab955 at 1/50 dilution. Cells were fixed with 100% methanol. Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-Tubulin antibody (rabbit mAb) Anti-beta IV Tubulin antibody [EPR16775] ab179504 - AlexaFluor^®594 Goat anti- Rabbit secondary Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 at 1/500 dilution (red). Nuclear DNA was labelled with DAPI (blue).

  Confocal image showing cytoplasmic staining in THP-1 cells

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)

  CD68 immunohistochemistry staining of human liver using mouse anti-CD68 antibody
  
  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue labelling CD68 with ab955 at 1/3000 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 a Goat Anti-mouse IgG H&L (HRP) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  Cytoplasmic staining on Kupffer cells of human liver (PMID: 12118106).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)

  CD68 immunohistochemistry staining of human tonsil using mouse anti-CD68 antibody
  
  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue labelling CD68 with ab955 at 1/3000 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 a Goat Anti-mouse IgG H&L (HRP) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
  

  Cytoplasmic staining on macrophages of human tonsil (PMID: 19543531).

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  Image courtesy of an anonymous customer review.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)

  CD68 immunohistochemistry staining of human liver using mouse anti-CD68 antibody
  
  ab955 staining CD68 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

  Tissue was fixed with paraformaldehyde. Samples were then blocked with 10% serum for 3 hours at 22°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa-Fluor^® 568 conjugated goat anti-mouse polyclonal was used as secondary antibody at a 1/400 dilution.

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  This image is courtesy of an anonymous customer review

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)

  ab955 staining CD68 in human ulcerated oral (Mucosa/Bone) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
  

  Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton-X 100 in PBS and blocked with 2.5% serum for 90 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/500 in 1% serum in PBS +0.01% Triton-X 100) for 16 hours at 4°C. A commercial IHC kit and DAB was used to visualize the staining.

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  Western blot - Anti-CD68 antibody [KP1] (ab955)

  Blocking and diluting buffer and concentration: 5% NFDM /TBST
  
  Exposure time: Lane1: 60 seconds; Lane2: 5 seconds; Lane3: 180 seconds; Lane4: 7 seconds
  
  Anti-CD68 antibody [RM1031] ab303565 works better than ab955 in western blot testing.
  
  We suggest optimizing experimental protocols (increasing lysate amount, using lower dilution or higher sensitivity ECL substrate) to improve results when using ab955 in western blot.

  Lanes 1 and 3: Western blot - Anti-CD68 antibody [KP1] (ab955) at 1/1000 dilution

  Lanes 2 and 4: Western blot - Anti-CD68 antibody [RM1031] (Anti-CD68 antibody [RM1031] ab303565) at 1/1000 dilution

  Lanes 1 - 2: Human spleen tissue lysate at 20 µg

  Lanes 3 - 4: THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Predicted band size: 37 kDa

  Observed band size: 110 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)

  Immunohistochemical analysis of formalin fixed paraffin embedded human liver labelling CD68 with ab955 at a concentration of 0.1 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.

  ab955 Anti-CD68 antibody [KP1] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

  Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)