AB199000

Anti-CD68 antibody [KP1 + C68/684]

Lab Essentials
What is this?

Be the first to review this product! Submit a review

(4 Publications)

Mouse Multiclonal CD68 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing CD68 protein.

View Alternative Names

CD68, Macrosialin, Gp110

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1 + C68/684] (AB199000)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1 + C68/684] (AB199000)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD68 with ab199000 at 1μg/ml (10X).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1 + C68/684] (AB199000)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human histiocytoma tissue labelling CD68 with ab199000 at 2 μg/mL for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.

Key facts

Host species

Mouse

Clonality

Multiclonal

Clone number

KP1 + C68/684

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Cell preparation containing CD68 protein. The exact immunogen used to generate this antibody is proprietary information.

P34810

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

What is an oligoclonal antibody?
This oligoclonal antibody is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 99% PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.

Biological function summary

CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.

Pathways

CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.

CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

See full target information CD68

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 13:936164 PubMed35990658

2022

Mice with humanized immune system as novel models to study HIV-associated pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Valerie J Rodriguez-Irizarry,Alina C Schneider,Daniel Ahle,Justin M Smith,Edu B Suarez-Martinez,Ethan A Salazar,Brianyell McDaniel Mims,Fahmida Rasha,Hanna Moussa,Naima Moustaïd-Moussa,Kevin Pruitt,Marcelo Fonseca,Mauricio Henriquez,Matthias A Clauss,Matthew B Grisham,Sharilyn Almodovar

Cancer immunology, immunotherapy : CII 68:1501-1513 PubMed31489465

2019

Tumor-induced peripheral immunosuppression promotes brain metastasis in patients with non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuping D Li,Jonathan B Lamano,Jason B Lamano,Jessica Quaggin-Smith,Dorina Veliceasa,Gurvinder Kaur,Dauren Biyashev,Dusten Unruh,Orin Bloch

Cancer immunology, immunotherapy : CII 68:999-1009 PubMed31030234

2019

Systemic and local immunosuppression in patients with high-grade meningiomas.

Applications

Unspecified application

Species

Unspecified reactive species

Yuping D Li,Dorina Veliceasa,Jason B Lamano,Jonathan B Lamano,Gurvinder Kaur,Dauren Biyashev,Craig M Horbinski,Tim J Kruser,Orin Bloch

Clinical cancer research : an official journal of 25:3643-3657 PubMed30824583

2019

Glioblastoma-Derived IL6 Induces Immunosuppressive Peripheral Myeloid Cell PD-L1 and Promotes Tumor Growth.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan B Lamano,Jason Balquidera Lamano,Yuping D Li,Joseph D DiDomenico,Winward Choy,Dorina Veliceasa,Daniel E Oyon,Shayan Fakurnejad,Leonel Ampie,Kartik Kesavabhotla,Rajwant Kaur,Gurvinder Kaur,Dauren Biyashev,Dusten J Unruh,Craig M Horbinski,C David James,Andrew T Parsa,Orin Bloch

View all publications

style

left-column

style

left-column

style

right-column

Alternative Products

Primary Antibodies

AB53444

Anti-CD68 antibody [FA-11]

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [FA-11] (AB53444)

Primary Antibodies

AB222914

Alexa Fluor® 488 Anti-CD68 antibody [KP1]

Recombinant|Advanced Validation

Multiplex immunohistochemistry - Alexa Fluor® 488 Anti-CD68 antibody [KP1] (AB222914)

Primary Antibodies

AB277938

Alexa Fluor® 594 Anti-CD68 antibody [EPR20545]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD68 antibody [EPR20545] (AB277938)

Primary Antibodies

AB224029

Alexa Fluor® 647 Anti-CD68 antibody [EPR20545]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD68 antibody [EPR20545] (AB224029)

Primary Antibodies

AB201844

Alexa Fluor® 488 Anti-CD68 antibody [FA-11]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD68 antibody [FA-11] (AB201844)

Primary Antibodies

AB201845

Alexa Fluor® 647 Anti-CD68 antibody [FA-11]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD68 antibody [FA-11] (AB201845)

Primary Antibodies

AB227458

Anti-CD68 antibody [EPR20545] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - BSA and Azide free (AB227458)

Primary Antibodies

AB213363

Anti-CD68 antibody [EPR20545]

Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] (AB213363)

Complementary Products

Assay Kits

AB287167

Human CD68 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD68 ELISA Kit (AB287167)

Primary Antibodies

AB182422

Anti-CD163 antibody [EPR19518]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Flow Cytometry - Anti-CD163 antibody [EPR19518] (AB182422)

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Primary Antibodies

AB64693

Anti-Mannose Receptor antibody

BOND RX™ Validated

Western blot - Anti-Mannose Receptor antibody (AB64693)

Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com