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AB845

Anti-CD68 antibody [KP1], prediluted

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(15 Publications)

Mouse Monoclonal CD68 antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 15 publications.

View Alternative Names

CD68, Macrosialin, Gp110

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1], prediluted (AB845)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1], prediluted (AB845)

Formalin-fixed, paraffin-embeded human tonsil tissue stained for CD68 using ab845 in immunohistochemical analysis. Antigen retrieval with EDTA buffer pH 8.0

Flow Cytometry - Anti-CD68 antibody [KP1], prediluted (AB845)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD68 antibody [KP1], prediluted (AB845)

Overlay histogram showing THP1 cells stained with ab845 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab845, 1/4 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

KP1

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P, Flow Cyt

applications

Specificity

This antibody is specific to macrophages in a wide variety of human tissues. It reacts with myeloid precursors and peripheral blood granulocytes. It also stains a cell population known as Plasmacytoid T cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/4", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification notes
Purified from TCS
Storage buffer
pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.
Biological function summary

CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.

Pathways

CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.

CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
See full target information CD68
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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 5:101556 PubMed38776872

2024

Transcriptomic and spatial dissection of human ex vivo right atrial tissue reveals proinflammatory microvascular changes in ischemic heart disease.

Applications

Unspecified application

Species

Unspecified reactive species

Suvi Linna-Kuosmanen,Eloi Schmauch,Kyriakitsa Galani,Johannes Ojanen,Carles A Boix,Tiit Örd,Anu Toropainen,Prosanta K Singha,Pierre R Moreau,Kristiina Harju,Adriana Blazeski,Åsa Segerstolpe,Veikko Lahtinen,Lei Hou,Kai Kang,Elamaran Meibalan,Leandro Z Agudelo,Hannu Kokki,Jari Halonen,Juho Jalkanen,Jarmo Gunn,Calum A MacRae,Maija Hollmén,Juha E K Hartikainen,Minna U Kaikkonen,Guillermo García-Cardeña,Pasi Tavi,Tuomas Kiviniemi,Manolis Kellis

Cancers 13: PubMed34885098

2021

Stage I-IV Colorectal Cancer Prognosis Can Be Predicted by Type and Number of Intratumoral Macrophages and CLEVER-1 Vessel Density.

Applications

Unspecified application

Species

Unspecified reactive species

Annika Ålgars,Lotta Kemppinen,Ruth Fair-Mäkelä,Harri Mustonen,Caj Haglund,Sirpa Jalkanen

International journal of molecular sciences 21: PubMed33050010

2020

Prognostic Role of Survivin and Macrophage Infiltration Quantified on Protein and mRNA Level in Molecular Subtypes Determined by RT-qPCR of , , and in Muscle-Invasive Bladder Cancer Treated by Adjuvant Chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Thorsten H Ecke,Adisch Kiani,Thorsten Schlomm,Frank Friedersdorff,Anja Rabien,Klaus Jung,Ergin Kilic,Peter Boström,Minna Tervahartiala,Pekka Taimen,Jan Gleichenhagen,Georg Johnen,Thomas Brüning,Stefan Koch,Jenny Roggisch,Ralph M Wirtz

The Prostate 79:1053-1058 PubMed30958903

2019

Protein kinase D1 induces G1-phase cell-cycle arrest independent of Checkpoint kinases by phosphorylating Cell Division Cycle Phosphatase 25.

Applications

Unspecified application

Species

Unspecified reactive species

Bita Nickkholgh,Sivanandane Sittadjody,Karina Ordonez,Michael Bryan Rothberg,K C Balaji

Oncotarget 9:25294-25303 PubMed29861872

2018

Prognostic roles of tumor associated macrophages in bladder cancer: a system review and meta-analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Shui-Qing Wu,Ran Xu,Xue-Feng Li,Xiao-Kun Zhao,Bin-Zhi Qian

Experimental neurology 305:76-88 PubMed29596844

2018

Molecular pathology of Multiple Sclerosis lesions reveals a heterogeneous expression pattern of genes involved in oligodendrogliogenesis.

Applications

IHC-Fr

Species

Human

T Zeis,O W Howell,R Reynolds,N Schaeren-Wiemers

The Journal of allergy and clinical immunology 141:1479-1482.e6 PubMed29225085

2017

Anticytokine autoantibodies in a patient with a heterozygous NFKB2 mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Kesava A Ramakrishnan,William Rae,Gabriela Barcenas-Morales,Yifang Gao,Reuben J Pengelly,Sanjay V Patel,Dinakantha S Kumararatne,Sarah Ennis,Rainer Döffinger,Saul N Faust,Anthony P Williams

Scientific reports 7:12682 PubMed28978923

2017

Immunological tumor status may predict response to neoadjuvant chemotherapy and outcome after radical cystectomy in bladder cancer.

Applications

IHC-P

Species

Human

Minna Tervahartiala,Pekka Taimen,Tuomas Mirtti,Ilmari Koskinen,Thorsten Ecke,Sirpa Jalkanen,Peter J Boström

Oncology reports 38:886-898 PubMed28656201

2017

Intratumoral and peritumoral expression of CD68 and CD206 in hepatocellular carcinoma and their prognostic value.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Xia Ren,Rui-Xue Leng,Yin-Guang Fan,Hai-Feng Pan,Bao-Zhu Li,Chang-Hao Wu,Qiang Wu,Na-Na Wang,Qi-Ru Xiong,Xiao-Ping Geng,Dong-Qing Ye

PloS one 10:e0133552 PubMed26197470

2015

Tumor-Associated Macrophages Provide Significant Prognostic Information in Urothelial Bladder Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Minna M Boström,Heikki Irjala,Tuomas Mirtti,Pekka Taimen,Tommi Kauko,Annika Ålgars,Sirpa Jalkanen,Peter J Boström
View all publications
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