AB844

Anti-CD68 antibody [PG-M1], prediluted

Name: Anti-CD68 antibody [PG-M1], prediluted (ab844) | Abcam
Brand: Abcam Limited
SKU: AB844
Price: 400 USD
Availability: InStock
Rating: 5 (1 reviews)

Lab Essentials
What is this?

(1 Review)

(3 Publications )

Mouse Monoclonal CD68 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications.

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1], prediluted (AB844)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1], prediluted (AB844)

ab844 staining CD68 in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry. Staining was detected using DAB.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

PG-M1

Isotype

IgG3

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Specificity

This antibody reacts with a glycoprotein of 110 kDa, expressed as intracytoplasmic molecule. It stains macrophages in a wide variety of tissues. Myeloid precursor cells and peripheral granulocytes are negative. This antibody does not react with granulocytes and their precursor cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.

Biological function summary

CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.

Pathways

CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.

CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

See full target information CD68

Alternative Names

CD68, Macrosialin, Gp110

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Publications (3)

Recent publications for all applications. Explore the full listand refine your search

International journal of cell biology 2023:6840271 PubMed37305351

2023

AhR and HIF-1 Signaling Pathways in Benign Meningioma under Hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Maria L Perepechaeva,Lyubov S Klyushova,Alevtina Y Grishanova

Oncology letters 21:372 PubMed33777196

2021

Secreted phosphoprotein-1 accelerates the progression of human colorectal cancer through activating β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jianzhong Yi,Yungeng Liu,Lei Zhang,Chuanfa Fang

The Journal of investigative dermatology 134:2598-2609 PubMed24714202

2014

Upregulation of MMP12 and its activity by UVA1 in human skin: potential implications for photoaging.

Applications

Species

Unspecified reactive species

Angela Tewari,Katarzyna Grys,Jutta Kollet,Robert Sarkany,Antony R Young

View all publications

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Product promise

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