Name: Anti-CD68 antibody [RM1031] - BSA and Azide free
SKU: ab303566
Rating: 5 (1 reviews)

Rabbit Recombinant Multiclonal CD68 antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr, ICC/IF and reacts with Human, Mouse, Rat samples.

Images

Western blot - Anti-CD68 antibody [RM1031] - BSA and Azide free (AB303566), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Multiclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	IHC-Fr	ICC/IF	Flow Cyt (Intra)	IP
Human	Tested	Tested	Expected	Tested	Not recommended	Not recommended
Mouse	Tested	Tested	Tested	Tested	Not recommended	Not recommended
Rat	Tested	Tested	Tested	Expected	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Target data

See full target information CD68

Function

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

Additional Targets

Cd68

Alternative names

CD68, Macrosialin, Gp110

Recommended products

Rabbit Recombinant Multiclonal CD68 antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr, ICC/IF and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Multiclonal
Immunogens: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: RM1031
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.

Biological function summary

CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.

Pathways

CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.

Associated diseases and disorders

CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

Western blot - Anti-CD68 antibody [RM1031] - BSA and Azide free (ab303566)
This data was developed using Anti-CD68 antibody [RM1031] ab303565, the same antibody clone in a different buffer formulation.
Western blot: Anti-CD68 antibody [RM1031] (Anti-CD68 antibody [RM1031] ab303565) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-CD68 antibody [RM1031] ab303565 was shown to bind specifically to CD68. A band was observed at 100 kDa in wild-type RAW 264.7 cell lysates with no signal observed at this size in CD68 knockout cell line. To generate this image, wild-type and CD68 knockout RAW 264.7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-CD68 antibody [RM1031] (Anti-CD68 antibody [RM1031] ab303565)
Lane 1: Wild-type RAW 264.7 cell lysate at 20 µg
Lane 2: CD68 knockout RAW 264.7 cell lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 100 kDa
Western blot - Anti-CD68 antibody [RM1031] - BSA and Azide free (ab303566)
This data was developed using Anti-CD68 antibody [RM1031] ab303565, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM /TBST

Exposure time: Lane1: 60 seconds; Lane2: 5 seconds; Lane3: 180 seconds; Lane4: 7 seconds

Anti-CD68 antibody [RM1031] ab303565 works better than Anti-CD68 antibody [KP1] ab955 in western blot testing.

We suggest optimizing experimental protocols (increasing lysate amount, using lower dilution or higher sensitivity ECL substrate) to improve results when using Anti-CD68 antibody [KP1] ab955 in western blot.
Lanes 1 and 3: Western blot - Anti-CD68 antibody [KP1] (Anti-CD68 antibody [KP1] ab955) at 1/1000 dilution
Lanes 2 and 4: Western blot - Anti-CD68 antibody [RM1031] (Anti-CD68 antibody [RM1031] ab303565) at 1/1000 dilution
Lanes 1 - 2: Human spleen tissue lysate at 20 µg
Lanes 3 - 4: THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 37 kDa
Observed band size: 110 kDa
Immunohistochemistry - Anti-CD68 antibody [RM1031] - BSA and Azide free (ab303566)
This data was developed using Anti-CD68 antibody [RM1031] ab303565, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CD68 with Anti-CD68 antibody [RM1031] ab303565 at 1/1000 (0.477 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection). Positve staining on the macrophages in the rat spleen. The section was incubated with Anti-CD68 antibody [RM1031] ab303565 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry - Anti-CD68 antibody [RM1031] - BSA and Azide free (ab303566)
This data was developed using Anti-CD68 antibody [RM1031] ab303565, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling CD68 with Anti-CD68 antibody [RM1031] ab303565 at 1/1000 (0.477 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection). Positve staining on the Kuffer cells in the rat liver. The section was incubated with Anti-CD68 antibody [RM1031] ab303565 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry - Anti-CD68 antibody [RM1031] - BSA and Azide free (ab303566)
This data was developed using Anti-CD68 antibody [RM1031] ab303565, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CD68 with Anti-CD68 antibody [RM1031] ab303565 at 1/1000 (0.477 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection). Positve staining on the Kuffer cells in the mouse liver. The section was incubated with Anti-CD68 antibody [RM1031] ab303565 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry - Anti-CD68 antibody [RM1031] - BSA and Azide free (ab303566)
This data was developed using Anti-CD68 antibody [RM1031] ab303565, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD68 with Anti-CD68 antibody [RM1031] ab303565 at 1/1000 (0.477 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection). Positve staining on the macrophages in the mouse spleen. The section was incubated with Anti-CD68 antibody [RM1031] ab303565 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Western blot - Anti-CD68 antibody [RM1031] - BSA and Azide free (ab303566)
This data was developed using Anti-CD68 antibody [RM1031] ab303565, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative controls: Jurkat, A20.
The molecular mass observed is consistent with the literature (PMID: 7680921)
Exposure time: 1 min
All lanes: Western blot - Anti-CD68 antibody [RM1031] (Anti-CD68 antibody [RM1031] ab303565) at 1/1000 dilution
Lane 1: Human spleen tissue lysate at 20 µg
Lane 2: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg
Lane 3: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg
Lane 4: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg
Lane 5: J774A.1 (mouse reticum cell sarcoma monocyte/macrophage) whole cell lysate at 20 µg
Lane 6: A20 (mouse reticum sarcoma B lymphocyte) whole cell lysate at 20 µg
Lane 7: NR8383 (rat alveolar macrophage) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 37 kDa
Observed band size: 110 kDa
Exposure time: 1min
Immunohistochemistry - Anti-CD68 antibody [RM1031] - BSA and Azide free (ab303566)
This data was developed using Anti-CD68 antibody [RM1031] ab303565, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD68 with Anti-CD68 antibody [RM1031] ab303565 at 1/1000 (0.477 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection). Positve staining on the macrophages in the human tonsil. The section was incubated with Anti-CD68 antibody [RM1031] ab303565 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Frozen sections) - Anti-CD68 antibody [RM1031] - BSA and Azide free (ab303566)
This data was developed using Anti-CD68 antibody [RM1031] ab303565, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat spleen (fresh) tissue labeling CD68 with Anti-CD68 antibody [RM1031] ab303565 at 1/50 (9.54 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining in the red pulp of rat spleen. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-CD68 antibody [RM1031] ab303565 for 60mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.<\p> .
Immunohistochemistry - Anti-CD68 antibody [RM1031] - BSA and Azide free (ab303566)
This data was developed using Anti-CD68 antibody [RM1031] ab303565, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CD68 with Anti-CD68 antibody [RM1031] ab303565 at 1/1000 (0.477 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection). Positve staining on the Kuffer cells in the human liver. The section was incubated with Anti-CD68 antibody [RM1031] ab303565 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Frozen sections) - Anti-CD68 antibody [RM1031] - BSA and Azide free (ab303566)
This data was developed using Anti-CD68 antibody [RM1031] ab303565, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 100% cold methanol-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen (fresh) tissue labeling CD68 with Anti-CD68 antibody [RM1031] ab303565 at 1/50 (9.54 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining in the red pulp of mouse spleen. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-CD68 antibody [RM1031] ab303565 for 60mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [RM1031] - BSA and Azide free (ab303566)
This data was developed using Anti-CD68 antibody [RM1031] ab303565, the same antibody clone in a different buffer formulation

Immunofluorescent analysis of 80% Methanol-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling CD68 with Anti-CD68 antibody [RM1031] ab303565 at 1/100 (4.77 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in THP-1 cell lineNegative control: Jurkat (PMID: 17583472 ? is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [RM1031] - BSA and Azide free (ab303566)
This data was developed using Anti-CD68 antibody [RM1031] ab303565, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage) cells labelling CD68 with Anti-CD68 antibody [RM1031] ab303565 at 1/100 (4.77 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in J774A.1 cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.