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AB134350

Anti-CD68 antibody [Y1/82A]

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(1 Publication)

Mouse Monoclonal CD68 antibody. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CD68.

View Alternative Names

CD68, Macrosialin, Gp110

1 Images
Flow Cytometry (Intracellular) - Anti-CD68 antibody [Y1/82A] (AB134350)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CD68 antibody [Y1/82A] (AB134350)

Intracellular immunofluorescent staining with flow cytometric analysis labelling CD68 in Human peripheral blood monocytes at 0.125 µg per million cells in 100 µl volume, intracellularly stained by ab134351.

  • 519 FITC

    FITC Anti-CD68 antibody [Y1/82A]

  • Biotin

    Biotin Anti-CD68 antibody [Y1/82A]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Y1/82A

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human CD68. The exact immunogen used to generate this antibody is proprietary information.

P34810

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Alternative Products

Primary Antibodies

AB53444

Anti-CD68 antibody [FA-11]

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [FA-11] (AB53444)

  • 4
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Primary Antibodies

AB222914

Alexa Fluor® 488 Anti-CD68 antibody [KP1]

Recombinant|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD68 antibody [KP1] (AB222914)

  • 4
  • 2
Primary Antibodies

AB277938

Alexa Fluor® 594 Anti-CD68 antibody [EPR20545]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD68 antibody [EPR20545] (AB277938)

  • 0
  • 0
Primary Antibodies

AB224029

Alexa Fluor® 647 Anti-CD68 antibody [EPR20545]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD68 antibody [EPR20545] (AB224029)

  • 0
  • 0
Primary Antibodies

AB201844

Alexa Fluor® 488 Anti-CD68 antibody [FA-11]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD68 antibody [FA-11] (AB201844)

  • 0
  • 0
Primary Antibodies

AB227458

Anti-CD68 antibody [EPR20545] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [EPR20545] - BSA and Azide free (AB227458)

  • 4
  • 1
Primary Antibodies

AB213363

Anti-CD68 antibody [EPR20545]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-CD68 antibody [EPR20545] (AB213363)

  • 4
  • 5

Complementary Products

Assay Kits

AB287167

Human CD68 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD68 ELISA Kit (AB287167)

  • 0
  • 0
Primary Antibodies

AB182422

Anti-CD163 antibody [EPR19518]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Flow Cytometry - Anti-CD163 antibody [EPR19518] (AB182422)

  • 5
  • 17
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB64693

Anti-Mannose Receptor antibody

BOND RX™ Validated

Western blot - Anti-Mannose Receptor antibody (AB64693)

  • 5
  • 42
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "0.125 µg/cells for 6 µg/cells", "FlowCytIntra-species-notes": "<p>in 100 μl volume.</p><p><a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified by affinity chromatography.
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.
Biological function summary

CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.

Pathways

CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.

CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
See full target information CD68
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:17707 PubMed37853206

2023

Use of transcranial low-intensity focused ultrasound for targeted delivery of stem cell-derived exosomes to the brain.

Applications

Unspecified application

Species

Unspecified reactive species

J Haroon,K Aboody,L Flores,M McDonald,K Mahdavi,M Zielinski,K Jordan,E Rindner,J Surya,V Venkatraman,V Go-Stevens,G Ngai,J Lara,C Hyde,S Schafer,M Schafer,A Bystritsky,I Nardi,T Kuhn,D Ross,S Jordan
View all publications
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Product promise

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