AB272739

Anti-CD69 antibody [EPR23147-45] - BSA and Azide free (Capture)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal CD69 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

CD69, CLEC2C, Early activation antigen CD69, Activation inducer molecule, BL-AC/P26, C-type lectin domain family 2 member C, EA1, Early T-cell activation antigen p60, GP32/28, Leukocyte surface antigen Leu-23, MLR-3, AIM

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Sandwich ELISA - Anti-CD69 antibody [EPR23147-45] - BSA and Azide free (Capture) (AB272739)

sELISA

Supplier Data

Sandwich ELISA - Anti-CD69 antibody [EPR23147-45] - BSA and Azide free (Capture) (AB272739)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab272464).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23147-45

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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Product details

ab272739 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human CD69. The recommended pair for sandwich ELISA is:
Capture: ab272739, Human CD69 Capture Antibody (unconjugated)
Detector: ab272740, Human CD69 Detector Antibody (unconjugated)
The reference range value is 93.75 - 6000 pg/ml.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: 100% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD69 also known as Activation-Induced C-type Lectin or Early T-cell Activation Antigen is a type II transmembrane protein with a mass of approximately 28-32 kDa. It is expressed mainly on activated leukocytes including T cells B cells and natural killer (NK) cells. Expression occurs rapidly after cell activation making CD69 a robust early marker used in flow cytometry analysis like with CD69 FITC or CD69 PE conjugates to study immune cell activation. The mouse version of CD69 serves as an important model for understanding its function in human biology.

Biological function summary

CD69 functions as a signaling molecule during the immune response influencing both cell proliferation and cytokine production. It is a member of the C-type lectin superfamily and operates as a homodimer on the cell surface. CD69 helps modulate the migration of T cells to inflammatory sites by affecting sphingosine-1-phosphate receptor 1 (S1P1) downregulation directing their tissue retention and primary response roles.

Pathways

The protein plays an important role in the regulation of the NF-kB and JAK-STAT signaling pathways. These are important for immune cell communication and response and CD69 interacts with proteins like the IL-2 receptor complex in these pathways. Additionally its cross-linking can lead to altered expression of cytokines and chemokines impacting overall immune function.

Altered CD69 expression links to conditions like autoimmune diseases and chronic inflammation. Its involvement in rheumatoid arthritis shows how CD69 may regulate abnormal immune responses. Furthermore the modulation of CD69 expression can be associated with cancer progression highlighting connections to proteins like FN50 and FN61 which participate in tumor immunity and metastasis control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transmembrane protein expressed mainly on T-cells resident in mucosa that plays an essential role in immune cell homeostasis. Rapidly expressed on the surface of platelets, T-lymphocytes and NK cells upon activation by various stimuli, such as antigen recognition or cytokine signaling, stimulates different signaling pathways in different cell types (PubMed : 24752896, PubMed : 26296369, PubMed : 35930205). Negatively regulates Th17 cell differentiation through its carbohydrate dependent interaction with galectin-1/LGALS1 present on immature dendritic cells (PubMed : 24752896). Association of CD69 cytoplasmic tail with the JAK3/STAT5 signaling pathway regulates the transcription of RORgamma/RORC and, consequently, differentiation toward the Th17 lineage (By similarity). Acts also via the S100A8/S100A9 complex present on peripheral blood mononuclear cells to promote the conversion of naive CD4 T-cells into regulatory T-cells (PubMed : 26296369). Acts as an oxidized low-density lipoprotein (oxLDL) receptor in CD4 T-lymphocytes and negatively regulates the inflammatory response by inducing the expression of PDCD1 through the activation of NFAT (PubMed : 35930205). Participates in adipose tissue-derived mesenchymal stem cells (ASCs)-mediated protection against P. aeruginosa infection. Mechanistically, specifically recognizes P. aeruginosa to promote ERK1 activation, followed by granulocyte-macrophage colony-stimulating factor (GM-CSF) and other inflammatory cytokines secretion (PubMed : 34841721). In eosinophils, induces IL-10 production through the ERK1/2 pathway (By similarity). Negatively regulates the chemotactic responses of effector lymphocytes and dendritic cells (DCs) to sphingosine 1 phosphate/S1P by acting as a S1PR1 receptor agonist and facilitating the internalization and degradation of the receptor (PubMed : 37039481).

See full target information CD69

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD69 antibody [EPR23147-45] - BSA and Azide free (Capture)

Sandwich ELISA - Anti-CD69 antibody [EPR23147-45] - BSA and Azide free (Capture) (AB272739)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Product promise