Mouse Recombinant Monoclonal CD69 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples.
Images
![Flow Cytometry - Anti-CD69 antibody [FN61] - BSA and Azide free (AB280967), expandable thumbnail](https://content.abcam.com/products/images/cd69-antibody-fn61-bsa-and-azide-free-ab280967--flow-cytometry-img181645.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD69 antibody [FN61] - BSA and Azide free (AB280967)")
![Flow Cytometry - Anti-CD69 antibody [FN61] - BSA and Azide free (AB280967), expandable thumbnail](https://content.abcam.com/products/images/cd69-antibody-fn61-bsa-and-azide-free-ab280967--flow-cytometry-img169901.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD69 antibody [FN61] - BSA and Azide free (AB280967)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| Flow Cyt | IHC-P | |
|---|---|---|
| Human | Tested | Not recommended |
| Mouse | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Transmembrane protein expressed mainly on T-cells resident in mucosa that plays an essential role in immune cell homeostasis. Rapidly expressed on the surface of platelets, T-lymphocytes and NK cells upon activation by various stimuli, such as antigen recognition or cytokine signaling, stimulates different signaling pathways in different cell types (PubMed:24752896, PubMed:26296369, PubMed:35930205). Negatively regulates Th17 cell differentiation through its carbohydrate dependent interaction with galectin-1/LGALS1 present on immature dendritic cells (PubMed:24752896). Association of CD69 cytoplasmic tail with the JAK3/STAT5 signaling pathway regulates the transcription of RORgamma/RORC and, consequently, differentiation toward the Th17 lineage (By similarity). Acts also via the S100A8/S100A9 complex present on peripheral blood mononuclear cells to promote the conversion of naive CD4 T-cells into regulatory T-cells (PubMed:26296369). Acts as an oxidized low-density lipoprotein (oxLDL) receptor in CD4 T-lymphocytes and negatively regulates the inflammatory response by inducing the expression of PDCD1 through the activation of NFAT (PubMed:35930205). Participates in adipose tissue-derived mesenchymal stem cells (ASCs)-mediated protection against P. aeruginosa infection. Mechanistically, specifically recognizes P. aeruginosa to promote ERK1 activation, followed by granulocyte-macrophage colony-stimulating factor (GM-CSF) and other inflammatory cytokines secretion (PubMed:34841721). In eosinophils, induces IL-10 production through the ERK1/2 pathway (By similarity). Negatively regulates the chemotactic responses of effector lymphocytes and dendritic cells (DCs) to sphingosine 1 phosphate/S1P by acting as a S1PR1 receptor agonist and facilitating the internalization and degradation of the receptor (PubMed:37039481).
Alternative names
CD69, CLEC2C, Early activation antigen CD69, Activation inducer molecule, BL-AC/P26, C-type lectin domain family 2 member C, EA1, Early T-cell activation antigen p60, GP32/28, Leukocyte surface antigen Leu-23, MLR-3, AIM
Recommended products
Mouse Recombinant Monoclonal CD69 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Carrier free
- Yes
- Clone number
- FN61
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody is unsuitable in Human, Mouse, and Rat for IHC-P as well as Mouse for Flow Cyt.
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab280967 is the carrier-free version of Anti-CD69 antibody [FN61] ab280965.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD69 also known as Activation-Induced C-type Lectin or Early T-cell Activation Antigen is a type II transmembrane protein with a mass of approximately 28-32 kDa. It is expressed mainly on activated leukocytes including T cells B cells and natural killer (NK) cells. Expression occurs rapidly after cell activation making CD69 a robust early marker used in flow cytometry analysis like with CD69 FITC or CD69 PE conjugates to study immune cell activation. The mouse version of CD69 serves as an important model for understanding its function in human biology.
- Biological function summary
CD69 functions as a signaling molecule during the immune response influencing both cell proliferation and cytokine production. It is a member of the C-type lectin superfamily and operates as a homodimer on the cell surface. CD69 helps modulate the migration of T cells to inflammatory sites by affecting sphingosine-1-phosphate receptor 1 (S1P1) downregulation directing their tissue retention and primary response roles.
- Pathways
The protein plays an important role in the regulation of the NF-kB and JAK-STAT signaling pathways. These are important for immune cell communication and response and CD69 interacts with proteins like the IL-2 receptor complex in these pathways. Additionally its cross-linking can lead to altered expression of cytokines and chemokines impacting overall immune function.
- Associated diseases and disorders
Altered CD69 expression links to conditions like autoimmune diseases and chronic inflammation. Its involvement in rheumatoid arthritis shows how CD69 may regulate abnormal immune responses. Furthermore the modulation of CD69 expression can be associated with cancer progression highlighting connections to proteins like FN50 and FN61 which participate in tumor immunity and metastasis control.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry - Anti-CD69 antibody [FN61] - BSA and Azide free (ab280967), expandable thumbnail](https://content.abcam.com/products/images/cd69-antibody-fn61-bsa-and-azide-free-ab280967--flow-cytometry-img181645.jpg "Flow Cytometry - Anti-CD69 antibody [FN61] - BSA and Azide free (ab280967)")
Flow Cytometry - Anti-CD69 antibody [FN61] - BSA and Azide free (ab280967)
This data was developed using Anti-CD69 antibody [FN61] ab280965 the same antibody clone in a different buffer formulation.Flow cytometric analysis of Daudi (Human Burkitt's lymphoma lymphoblast) treated with 50 ng/ml PMA for 24 hours (Red) / Untreated control (Green) cells labelling CD69 with Anti-CD69 antibody [FN61] ab280965 at 1/1000 dilution (0.1ug) (Red) and Green compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
![Flow Cytometry - Anti-CD69 antibody [FN61] - BSA and Azide free (ab280967), expandable thumbnail](https://content.abcam.com/products/images/cd69-antibody-fn61-bsa-and-azide-free-ab280967--flow-cytometry-img169901.jpg "Flow Cytometry - Anti-CD69 antibody [FN61] - BSA and Azide free (ab280967)")
Flow Cytometry - Anti-CD69 antibody [FN61] - BSA and Azide free (ab280967)
This data was developed using Anti-CD69 antibody [FN61] ab280965 the same antibody clone in a different buffer formulation.Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte) treated with 5ug/ml PHA for 24 hours (Red) / Untreated control (Green) cells labelling CD69 with Anti-CD69 antibody [FN61] ab280965 at 1/1000 dilution (0.1ug) (Red) and Green compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
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