JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB224194

Anti-CD7 antibody [EPR22065]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal CD7 antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human samples.

View Alternative Names

CD7, T-cell antigen CD7, GP40, T-cell leukemia antigen, T-cell surface antigen Leu-9, TP41

5 Images
Western blot - Anti-CD7 antibody [EPR22065] (AB224194)
  • WB

Supplier Data

Western blot - Anti-CD7 antibody [EPR22065] (AB224194)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times :

Lane 1 : 3 minutes

Lane 2 : 48 seconds

This blot was developed using a higher sensitivity ECL substrate.

The molecular mass observed is consistent with what has been described in the literature (PMID : 24157461, PMID : 2479685); the actual size different from the predicted is due to glycosylation (PMID : 2479685).

All lanes:

Western blot - Anti-CD7 antibody [EPR22065] (ab224194) at 1/1000 dilution

Lane 1:

Human tonsil lysate at 20 µg

Lane 2:

Human thymus lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 25 kDa

Observed band size: 37 kDa

false

Flow Cytometry - Anti-CD7 antibody [EPR22065] (AB224194)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD7 antibody [EPR22065] (AB224194)

Human PBMCs (peripheral blood mononuclear cells) were stained with Alexa Fluor® 647-conjugated anti-human CD3 and rabbit IgG - ab172730 (Left) or CD7 - ab224194 at 1/500 (Right). Goat anti-Rabbit IgG Fc (Dylight® 488, ab98462), at 1/2000 dilution was used as the secondary antibody. Data shown was gated on viable cells. The expression pattern of CD7 is consistent with literature. (PMID : 3260936)

Western blot - Anti-CD7 antibody [EPR22065] (AB224194)
  • WB

Supplier Data

Western blot - Anti-CD7 antibody [EPR22065] (AB224194)

Blocking and dilution buffer : 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

The molecular mass observed is consistent with what has been described in the literature (PMID : 24157461, PMID : 2479685); the actual size different from the predicted is due to glycosylation (PMID : 2479685).

Negative control : Raji (PMID : 1633296, PMID : 11389079).

All lanes:

Western blot - Anti-CD7 antibody [EPR22065] (ab224194) at 1/1000 dilution

Lane 1:

MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 20 µg

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 3:

Raji (human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 25 kDa

Observed band size: 37 kDa

false

Exposure time: 3min

Immunoprecipitation - Anti-CD7 antibody [EPR22065] (AB224194)
  • IP

Supplier Data

Immunoprecipitation - Anti-CD7 antibody [EPR22065] (AB224194)

CD7 was immunoprecipitated from 0.35 mg of human tonsil lysate with ab224194 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224194 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Human tonsil lysate 10 μg (Input).
Lane 2 : ab224194 IP in human tonsil lysate lysate (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab224194 in human tonsil lysate (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-CD7 antibody [EPR22065] (ab224194)

Predicted band size: 25 kDa

Observed band size: 37 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-CD7 antibody [EPR22065] (AB224194)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD7 antibody [EPR22065] (AB224194)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MOLT-4 (human lymphoblastic leukemia cell line) cells labeling CD7 with ab224194 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing Membranous staining in MOLT-4 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

PDS only control : Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Negative control : Raji (PMID : 16332967).

  • Carrier free

    Anti-CD7 antibody [EPR22065] - BSA and Azide free

  • 578 PE

    PE Anti-CD7 antibody [EPR22065]

  • 660 APC

    APC Anti-CD7 antibody [EPR22065]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22065

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, IP, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

You may be interested in:

AB284026

Human CD7 ELISA Kit

0

0 Reviews

View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD7 also known as Leu-9 or GP40 is a cell surface protein with a mass of approximately 40 kDa. The target is mainly expressed on T cells and NK cells and can be found during early T-cell development. Researchers also report CD7 expression in some B cell lineage progenitors. The CD7 function involves mediating cell signal transduction which contributes to the activation and differentiation of immune cells making it an important target in immunology studies.
Biological function summary

CD7 plays a role in modulating interactions between immune cells without being part of a larger complex. It bridges interactions promoting immune responses by influencing T cell proliferation and cytokine production. CD7 control is important in regulating immune response intensity and provides an essential checkpoint in immune system modulation. Variations in CD7 function can significantly influence immune cell behavior impacting host defense mechanisms.

Pathways

CD7 participates in immune signaling pathways such as the T-cell receptor (TCR) signaling pathway. This pathway is essential for T cell activation and involves proteins like CD3 and ZAP-70 which work together with CD7 to transduce activation signals. Alterations in the CD7 pathway can affect the downstream signaling events leading to changes in immune cell activation and function.

Aberrations in CD7 expression or function link to conditions like T-cell acute lymphoblastic leukemia (T-ALL) and certain autoimmune diseases. In T-ALL abnormal CD7 expression serves as an indicator for diagnosis and disease progression monitoring. CD7 also interacts with proteins such as CD45 and CD3 which play roles in leukemogenesis and immune system dysregulation. Studies also explore anti-CD7 strategies for therapeutic interventions aiming to modulate its activity for treating these conditions effectively.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Transmembrane glycoprotein expressed by T-cells and natural killer (NK) cells and their precursors (PubMed : 7506726). Plays a costimulatory role in T-cell activation upon binding to its ligand K12/SECTM1 (PubMed : 10652336). In turn, mediates the production of cytokines such as IL-2 (PubMed : 1709867). On resting NK-cells, CD7 activation results in a significant induction of interferon-gamma levels (PubMed : 7506726).
See full target information CD7
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB213014

Anti-CD7 antibody [124-1D1] - BSA and Azide free

primary-antibodies

cd7-antibody-124-1d1-bsa-and-azide-free-ab213014

0

(0 reviews)

Alternative Product
Primary Antibodies

AB18276

FITC Anti-CD7 antibody [MEM-186]

primary-antibodies

fitc-cd7-antibody-mem-186-ab18276

0

(0 reviews)

Alternative Product
Primary Antibodies

AB239284

APC Anti-CD7 antibody [MEM-186]

primary-antibodies

apc-cd7-antibody-mem-186-ab239284

0

(0 reviews)

Alternative Product
Primary Antibodies

AB239299

PerCP Anti-CD7 antibody [MEM-186]

primary-antibodies

percp-cd7-antibody-mem-186-ab239299

0

(0 reviews)

Alternative Product
Primary Antibodies

AB223560

APC Anti-CD7 antibody [124-1D1]

primary-antibodies

apc-cd7-antibody-124-1d1-ab223560

0

(0 reviews)

Alternative Product
Primary Antibodies

AB230468

FITC Anti-CD7 antibody [124-1D1]

primary-antibodies

fitc-cd7-antibody-124-1d1-ab230468

0

(0 reviews)

Alternative Product
Primary Antibodies

AB104872

Anti-CD7 antibody

primary-antibodies

cd7-antibody-ab104872

0

(0 reviews)

Alternative Product
Primary Antibodies

AB199023

Alexa Fluor® 647 Anti-CD7 antibody [EPR4242]

primary-antibodies

alexa-fluor-647-cd7-antibody-epr4242-ab199023

0

(0 reviews)

Alternative Product
Primary Antibodies

AB199022

Alexa Fluor® 488 Anti-CD7 antibody [EPR4242]

primary-antibodies

alexa-fluor-488-cd7-antibody-epr4242-ab199022

0

(0 reviews)

Alternative Product
Primary Antibodies

AB183325

Anti-CD7 antibody [SP94]

primary-antibodies

cd7-antibody-sp94-ab183325

0

(0 reviews)

Alternative Product
Primary Antibodies

AB230834

Anti-CD7 antibody [EPR4242] - BSA and Azide free

primary-antibodies

cd7-antibody-epr4242-bsa-and-azide-free-ab230834

5

(2 reviews)

Alternative Product
Primary Antibodies

AB230904

Anti-CD7 antibody [SP94] - BSA and Azide free

primary-antibodies

cd7-antibody-sp94-bsa-and-azide-free-ab230904

0

(0 reviews)

Alternative Product
Primary Antibodies

AB109296

Anti-CD7 antibody [EPR4242]

primary-antibodies

cd7-antibody-epr4242-ab109296

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com