AB224194

Anti-CD7 antibody [EPR22065]

20ul selling size
RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal CD7 antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CD7, T-cell antigen CD7, GP40, T-cell leukemia antigen, T-cell surface antigen Leu-9, TP41

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-CD7 antibody [EPR22065] (AB224194)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD7 antibody [EPR22065] (AB224194)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MOLT-4 (human lymphoblastic leukemia cell line) cells labeling CD7 with ab224194 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing Membranous staining in MOLT-4 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

PDS only control : Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Negative control : Raji (PMID : 16332967).

Flow Cytometry - Anti-CD7 antibody [EPR22065] (AB224194)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD7 antibody [EPR22065] (AB224194)

Human PBMCs (peripheral blood mononuclear cells) were stained with Alexa Fluor^® 647-conjugated anti-human CD3 and rabbit IgG - ab172730 (Left) or CD7 - ab224194 at 1/500 (Right). Goat anti-Rabbit IgG Fc (Dylight^® 488, ab98462), at 1/2000 dilution was used as the secondary antibody. Data shown was gated on viable cells. The expression pattern of CD7 is consistent with literature. (PMID : 3260936)

Supplier Data

Immunoprecipitation - Anti-CD7 antibody [EPR22065] (AB224194)

CD7 was immunoprecipitated from 0.35 mg of human tonsil lysate with ab224194 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224194 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Human tonsil lysate 10 μg (Input).
Lane 2 : ab224194 IP in human tonsil lysate lysate (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab224194 in human tonsil lysate (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-CD7 antibody [EPR22065] (ab224194)

Predicted band size: 25 kDa

Observed band size: 37 kDa

false

Supplier Data

Western blot - Anti-CD7 antibody [EPR22065] (AB224194)

Blocking and dilution buffer : 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

The molecular mass observed is consistent with what has been described in the literature (PMID : 24157461, PMID : 2479685); the actual size different from the predicted is due to glycosylation (PMID : 2479685).

Negative control : Raji (PMID : 1633296, PMID : 11389079).

All lanes:

Western blot - Anti-CD7 antibody [EPR22065] (ab224194) at 1/1000 dilution

Lane 1:

MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 20 µg

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 3:

Raji (human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 25 kDa

Observed band size: 37 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-CD7 antibody [EPR22065] (AB224194)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times :

Lane 1 : 3 minutes

Lane 2 : 48 seconds

This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Western blot - Anti-CD7 antibody [EPR22065] (ab224194) at 1/1000 dilution

Lane 1:

Human tonsil lysate at 20 µg

Lane 2:

Human thymus lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 25 kDa

Observed band size: 37 kDa

false

Carrier free

Anti-CD7 antibody [EPR22065] - BSA and Azide free
660 APC

APC Anti-CD7 antibody [EPR22065]
578 PE

PE Anti-CD7 antibody [EPR22065]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22065

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, Flow Cyt, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD7 also known as Leu-9 or GP40 is a cell surface protein with a mass of approximately 40 kDa. The target is mainly expressed on T cells and NK cells and can be found during early T-cell development. Researchers also report CD7 expression in some B cell lineage progenitors. The CD7 function involves mediating cell signal transduction which contributes to the activation and differentiation of immune cells making it an important target in immunology studies.

Biological function summary

CD7 plays a role in modulating interactions between immune cells without being part of a larger complex. It bridges interactions promoting immune responses by influencing T cell proliferation and cytokine production. CD7 control is important in regulating immune response intensity and provides an essential checkpoint in immune system modulation. Variations in CD7 function can significantly influence immune cell behavior impacting host defense mechanisms.

Pathways

CD7 participates in immune signaling pathways such as the T-cell receptor (TCR) signaling pathway. This pathway is essential for T cell activation and involves proteins like CD3 and ZAP-70 which work together with CD7 to transduce activation signals. Alterations in the CD7 pathway can affect the downstream signaling events leading to changes in immune cell activation and function.

Aberrations in CD7 expression or function link to conditions like T-cell acute lymphoblastic leukemia (T-ALL) and certain autoimmune diseases. In T-ALL abnormal CD7 expression serves as an indicator for diagnosis and disease progression monitoring. CD7 also interacts with proteins such as CD45 and CD3 which play roles in leukemogenesis and immune system dysregulation. Studies also explore anti-CD7 strategies for therapeutic interventions aiming to modulate its activity for treating these conditions effectively.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transmembrane glycoprotein expressed by T-cells and natural killer (NK) cells and their precursors (PubMed : 7506726). Plays a costimulatory role in T-cell activation upon binding to its ligand K12/SECTM1 (PubMed : 10652336). In turn, mediates the production of cytokines such as IL-2 (PubMed : 1709867). On resting NK-cells, CD7 activation results in a significant induction of interferon-gamma levels (PubMed : 7506726).

See full target information CD7

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

ACS omega 10:17416-17434 PubMed40352528

2025

Mercury Can Bond to α-Carbon of Curcumin Increasing Stability in Aqueous Medium and Demonstrated Selective Cytotoxicity against Acute Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Sougata Mondal,Upasana Das,Oyendrila Ghosh,Bidisha Maiti,Shuvam Halder,Uttam Pal,Kalyan Kusum Mukherjee,Supratim Ghosh

iMeta 3:e221 PubMed39135698

2024

Cell-type-specific expression analysis of liver transcriptomics with clinical parameters to decipher the cause of intrahepatic inflammation in chronic hepatitis B.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wang,Qian Li,Yuanwang Qiu,Simo Kitanovski,Chen Wang,Chenxia Zhang,Fahong Li,Xiaoguang Li,Zhenfeng Zhang,Lihua Huang,Jiming Zhang,Daniel Hoffmann,Mengji Lu,Hongzhou Lu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD7 antibody [EPR22065]

Immunocytochemistry/ Immunofluorescence - Anti-CD7 antibody [EPR22065] (AB224194)

Flow Cytometry - Anti-CD7 antibody [EPR22065] (AB224194)

Immunoprecipitation - Anti-CD7 antibody [EPR22065] (AB224194)

Western blot - Anti-CD7 antibody [EPR22065] (AB224194)

Western blot - Anti-CD7 antibody [EPR22065] (AB224194)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise