Skip to main content

Rabbit Recombinant Monoclonal CD7 antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Flow Cytometry - Anti-CD7 antibody [EPR22065] (AB224194), expandable thumbnail
  • Western blot - Anti-CD7 antibody [EPR22065] (AB224194), expandable thumbnail
  • Immunoprecipitation - Anti-CD7 antibody [EPR22065] (AB224194), expandable thumbnail
  • Western blot - Anti-CD7 antibody [EPR22065] (AB224194), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD7 antibody [EPR22065] (AB224194), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IF
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Transmembrane glycoprotein expressed by T-cells and natural killer (NK) cells and their precursors (PubMed:7506726). Plays a costimulatory role in T-cell activation upon binding to its ligand K12/SECTM1 (PubMed:10652336). In turn, mediates the production of cytokines such as IL-2 (PubMed:1709867). On resting NK-cells, CD7 activation results in a significant induction of interferon-gamma levels (PubMed:7506726).

Alternative names

T-cell antigen CD7, GP40, T-cell leukemia antigen, T-cell surface antigen Leu-9, TP41, CD7

Recommended products

Rabbit Recombinant Monoclonal CD7 antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR22065

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD7 also known as Leu-9 or GP40 is a cell surface protein with a mass of approximately 40 kDa. The target is mainly expressed on T cells and NK cells and can be found during early T-cell development. Researchers also report CD7 expression in some B cell lineage progenitors. The CD7 function involves mediating cell signal transduction which contributes to the activation and differentiation of immune cells making it an important target in immunology studies.

Biological function summary

CD7 plays a role in modulating interactions between immune cells without being part of a larger complex. It bridges interactions promoting immune responses by influencing T cell proliferation and cytokine production. CD7 control is important in regulating immune response intensity and provides an essential checkpoint in immune system modulation. Variations in CD7 function can significantly influence immune cell behavior impacting host defense mechanisms.

Pathways

CD7 participates in immune signaling pathways such as the T-cell receptor (TCR) signaling pathway. This pathway is essential for T cell activation and involves proteins like CD3 and ZAP-70 which work together with CD7 to transduce activation signals. Alterations in the CD7 pathway can affect the downstream signaling events leading to changes in immune cell activation and function.

Associated diseases and disorders

Aberrations in CD7 expression or function link to conditions like T-cell acute lymphoblastic leukemia (T-ALL) and certain autoimmune diseases. In T-ALL abnormal CD7 expression serves as an indicator for diagnosis and disease progression monitoring. CD7 also interacts with proteins such as CD45 and CD3 which play roles in leukemogenesis and immune system dysregulation. Studies also explore anti-CD7 strategies for therapeutic interventions aiming to modulate its activity for treating these conditions effectively.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Flow Cytometry - Anti-CD7 antibody [EPR22065] (ab224194), expandable thumbnail

    Flow Cytometry - Anti-CD7 antibody [EPR22065] (ab224194)

    Human PBMCs (peripheral blood mononuclear cells) were stained with Alexa Fluor® 647-conjugated anti-human CD3 and rabbit IgG - Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 (Left) or CD7 - ab224194 at 1/500 (Right). Goat anti-Rabbit IgG Fc (Dylight® 488, ab98462), at 1/2000 dilution was used as the secondary antibody. Data shown was gated on viable cells. The expression pattern of CD7 is consistent with literature. (PMID: 3260936)

  • Western blot - Anti-CD7 antibody [EPR22065] (ab224194), expandable thumbnail

    Western blot - Anti-CD7 antibody [EPR22065] (ab224194)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times:

    Lane 1: 3 minutes

    Lane 2: 48 seconds

    This blot was developed using a higher sensitivity ECL substrate.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 24157461, PMID: 2479685); the actual size different from the predicted is due to glycosylation (PMID: 2479685).

    All lanes: Western blot - Anti-CD7 antibody [EPR22065] (ab224194) at 1/1000 dilution

    Lane 1: Human tonsil lysate at 20 µg

    Lane 2: Human thymus lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 25 kDa

    Observed band size: 37 kDa

  • Immunoprecipitation - Anti-CD7 antibody [EPR22065] (ab224194), expandable thumbnail

    Immunoprecipitation - Anti-CD7 antibody [EPR22065] (ab224194)

    CD7 was immunoprecipitated from 0.35 mg of human tonsil lysate with ab224194 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224194 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Human tonsil lysate 10 μg (Input).
    Lane 2: ab224194 IP in human tonsil lysate lysate (+).
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab224194 in human tonsil lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    All lanes: Immunoprecipitation - Anti-CD7 antibody [EPR22065] (ab224194)

    Predicted band size: 25 kDa

    Observed band size: 37 kDa

  • Western blot - Anti-CD7 antibody [EPR22065] (ab224194), expandable thumbnail

    Western blot - Anti-CD7 antibody [EPR22065] (ab224194)

    Blocking and dilution buffer: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 24157461, PMID: 2479685); the actual size different from the predicted is due to glycosylation (PMID: 2479685).

    Negative control: Raji (PMID: 1633296, PMID: 11389079).

    All lanes: Western blot - Anti-CD7 antibody [EPR22065] (ab224194) at 1/1000 dilution

    Lane 1: MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 20 µg

    Lane 2: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

    Lane 3: Raji (human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 25 kDa

    Observed band size: 37 kDa

    Exposure time: 3min

  • Immunocytochemistry/ Immunofluorescence - Anti-CD7 antibody [EPR22065] (ab224194), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD7 antibody [EPR22065] (ab224194)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MOLT-4 (human lymphoblastic leukemia cell line) cells labeling CD7 with ab224194 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing Membranous staining in MOLT-4 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).

    PDS only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

    Negative control: Raji (PMID: 16332967).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com