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Anti-CD7 antibody [EPR4242] (ab109296) is a rabbit monoclonal antibody that is used to detect CD7 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

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Images

Western blot - Anti-CD7 antibody [EPR4242] (AB109296), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-CD7 antibody [EPR4242] (AB109296), expandable thumbnail
  • Immunoprecipitation - Anti-CD7 antibody [EPR4242] (AB109296), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD7 antibody [EPR4242] (AB109296), expandable thumbnail
  • OI-RD Scanning - Anti-CD7 antibody [EPR4242] (AB109296), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Expected
Expected
Rat
Tested
Expected
Expected
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/8000
Notes

For unpurified use at 1/1600.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/8000
Notes

For unpurified use at 1/1600.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Human
Dilution info
1/8000
Notes

For unpurified use at 1/1600.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/50
Notes

For unpurified use at 1/100 - 1/250.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

11 products for Alternative Product

Target data

Function

Transmembrane glycoprotein expressed by T-cells and natural killer (NK) cells and their precursors (PubMed:7506726). Plays a costimulatory role in T-cell activation upon binding to its ligand K12/SECTM1 (PubMed:10652336). In turn, mediates the production of cytokines such as IL-2 (PubMed:1709867). On resting NK-cells, CD7 activation results in a significant induction of interferon-gamma levels (PubMed:7506726).

Alternative names

CD7, T-cell antigen CD7, GP40, T-cell leukemia antigen, T-cell surface antigen Leu-9, TP41

Recommended products

Anti-CD7 antibody [EPR4242] (ab109296) is a rabbit monoclonal antibody that is used to detect CD7 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4242
Purification technique
Affinity purification Protein A
Specificity

The mouse and rat recommendation is based on the IHC-P results. We do not guarantee WB for mouse and rat.

Dissociation constant
2.4 x 10-12 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-CD7 antibody [EPR4242] (ab109296) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), in Human, Mouse, Rat samples.

What is the molecular weight of CD7?


Anti-CD7 [EPR4242] (ab109296) specifically detects a band for CD7 (UniProt: P09564) at a molecular weight of 25kDa.

Recommended positive controls


Flow Cyt (intra): Jurkat cells; IP: Human spleen lysate; ICC/IF: Jurkat cells; IHC-P: Rat and mouse spleen tissues. Human colon carcinoma and tonsil tissues;WB: Human spleen lysate,

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products


We have a range of other formats of antibody clone [EPR4242] also available for your convenience:
ab109296, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-CD7 antibody [EPR4242] ab199022, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-CD7 antibody [EPR4242] ab199023, Carrier free - Anti-CD7 antibody [EPR4242] - BSA and Azide free ab230834, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-CD7 antibody [EPR4242] ab277233, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-CD7 antibody [EPR4242] ab279334, PE - PE Anti-CD7 antibody [EPR4242] ab303140, APC - APC Anti-CD7 antibody [EPR4242] ab303141, HRP - HRP Anti-CD7 antibody [EPR4242] ab303142, Alkaline Phosphatase - Alkaline Phosphatase Anti-CD7 antibody [EPR4242] ab308876, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-CD7 antibody [EPR4242] ab312617, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-CD7 antibody [EPR4242] ab320942



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD7 also known as Leu-9 or GP40 is a cell surface protein with a mass of approximately 40 kDa. The target is mainly expressed on T cells and NK cells and can be found during early T-cell development. Researchers also report CD7 expression in some B cell lineage progenitors. The CD7 function involves mediating cell signal transduction which contributes to the activation and differentiation of immune cells making it an important target in immunology studies.

Biological function summary

CD7 plays a role in modulating interactions between immune cells without being part of a larger complex. It bridges interactions promoting immune responses by influencing T cell proliferation and cytokine production. CD7 control is important in regulating immune response intensity and provides an essential checkpoint in immune system modulation. Variations in CD7 function can significantly influence immune cell behavior impacting host defense mechanisms.

Pathways

CD7 participates in immune signaling pathways such as the T-cell receptor (TCR) signaling pathway. This pathway is essential for T cell activation and involves proteins like CD3 and ZAP-70 which work together with CD7 to transduce activation signals. Alterations in the CD7 pathway can affect the downstream signaling events leading to changes in immune cell activation and function.

Associated diseases and disorders

Aberrations in CD7 expression or function link to conditions like T-cell acute lymphoblastic leukemia (T-ALL) and certain autoimmune diseases. In T-ALL abnormal CD7 expression serves as an indicator for diagnosis and disease progression monitoring. CD7 also interacts with proteins such as CD45 and CD3 which play roles in leukemogenesis and immune system dysregulation. Studies also explore anti-CD7 strategies for therapeutic interventions aiming to modulate its activity for treating these conditions effectively.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-CD7 antibody [EPR4242] (ab109296), expandable thumbnail

    Western blot - Anti-CD7 antibody [EPR4242] (ab109296)

    The molecular mass observed is consistent with what has been described in the literatures (PMID: 24157461, 2479685).

    All lanes: Western blot - Anti-CD7 antibody [EPR4242] (ab109296) at 1/10000 dilution

    All lanes: Human Spleen Lysate at 15 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 25 kDa

    Observed band size: 37 kDa

  • Flow Cytometry (Intracellular) - Anti-CD7 antibody [EPR4242] (ab109296), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CD7 antibody [EPR4242] (ab109296)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte) cells labelling CD7 with purified ab109296 at 1/100 dilution (1μg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-CD7 antibody [EPR4242] (ab109296), expandable thumbnail

    Immunoprecipitation - Anti-CD7 antibody [EPR4242] (ab109296)

    CD7 was immunoprecipitated from 0.35 mg Human spleen lysate 10 ug with ab109296 at 1/50. Western blot was performed on the immunoprecipitate using ab109296 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: Human spleen lysate 10 ug

    Lane 2: ab109296 IP in Human spleen lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab109296 in Human spleen lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

    All lanes: Immunoprecipitation - Anti-CD7 antibody [EPR4242] (ab109296)

    Predicted band size: 25 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CD7 antibody [EPR4242] (ab109296), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD7 antibody [EPR4242] (ab109296)

    Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling CD7 with Purified ab109296 at 1/50 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • OI-RD Scanning - Anti-CD7 antibody [EPR4242] (ab109296), expandable thumbnail

    OI-RD Scanning - Anti-CD7 antibody [EPR4242] (ab109296)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] (ab109296), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] (ab109296)

    Tissue Microarrays stained for "Anti-CD7 antibody [EPR4242]" using "ab109296"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab109296 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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