Anti-CD7 antibody [EPR4242]

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CD7 antibody [EPR4242] (AB109296)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte) cells labelling CD7 with purified ab109296 at 1/100 dilution (1μg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CD7 antibody [EPR4242] (AB109296)

Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling CD7 with Purified ab109296 at 1/50 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] (AB109296)

Tissue Microarrays stained for "Anti-CD7 antibody [EPR4242]" using "ab109296"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab109296 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• IP

Unknown

Immunoprecipitation - Anti-CD7 antibody [EPR4242] (AB109296)

CD7 was immunoprecipitated from 0.35 mg Human spleen lysate 10 ug with ab109296 at 1/50. Western blot was performed on the immunoprecipitate using ab109296 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Human spleen lysate 10 ug

Lane 2 : ab109296 IP in Human spleen lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab109296 in Human spleen lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds

All lanes:

Immunoprecipitation - Anti-CD7 antibody [EPR4242] (ab109296)

Predicted band size: 25 kDa

false

• WB

Lab

Western blot - Anti-CD7 antibody [EPR4242] (AB109296)

The molecular mass observed is consistent with what has been described in the literatures (PMID : 24157461, 2479685).

All lanes:

Western blot - Anti-CD7 antibody [EPR4242] (ab109296) at 1/10000 dilution

All lanes:

Human Spleen Lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 25 kDa

Observed band size: 37 kDa

false

• OI-RD Scanning

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OI-RD Scanning - Anti-CD7 antibody [EPR4242] (AB109296)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.