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Rabbit Recombinant Monoclonal CD7 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.


Images

Western blot - Anti-CD7 antibody [EPR4242] - BSA and Azide free (AB230834), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-CD7 antibody [EPR4242] - BSA and Azide free (AB230834), expandable thumbnail
  • Immunoprecipitation - Anti-CD7 antibody [EPR4242] - BSA and Azide free (AB230834), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD7 antibody [EPR4242] - BSA and Azide free (AB230834), expandable thumbnail
  • OI-RD Scanning - Anti-CD7 antibody [EPR4242] - BSA and Azide free (AB230834), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Expected
Expected
Rat
Tested
Expected
Expected
Expected
Expected

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

14 products for Alternative Product

8 products for Alternative Version

Target data

Function

Not yet known.

Alternative names

T-cell antigen CD7, GP40, T-cell leukemia antigen, T-cell surface antigen Leu-9, TP41, CD7

Recommended products

Rabbit Recombinant Monoclonal CD7 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

Alternative names

T-cell antigen CD7, GP40, T-cell leukemia antigen, T-cell surface antigen Leu-9, TP41, CD7

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR4242

Purification technique

Affinity purification Protein A

Specificity

The mouse and rat recommendation is based on the IHC-P results. We do not guarantee WB for mouse and rat.

Dissociation constant

2.4 x 10-12 M

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab230834 is the carrier-free version of Anti-CD7 antibody [EPR4242] ab109296.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD7 also known as Leu-9 or GP40 is a cell surface protein with a mass of approximately 40 kDa. The target is mainly expressed on T cells and NK cells and can be found during early T-cell development. Researchers also report CD7 expression in some B cell lineage progenitors. The CD7 function involves mediating cell signal transduction which contributes to the activation and differentiation of immune cells making it an important target in immunology studies.

Biological function summary

CD7 plays a role in modulating interactions between immune cells without being part of a larger complex. It bridges interactions promoting immune responses by influencing T cell proliferation and cytokine production. CD7 control is important in regulating immune response intensity and provides an essential checkpoint in immune system modulation. Variations in CD7 function can significantly influence immune cell behavior impacting host defense mechanisms.

Pathways

CD7 participates in immune signaling pathways such as the T-cell receptor (TCR) signaling pathway. This pathway is essential for T cell activation and involves proteins like CD3 and ZAP-70 which work together with CD7 to transduce activation signals. Alterations in the CD7 pathway can affect the downstream signaling events leading to changes in immune cell activation and function.

Associated diseases and disorders

Aberrations in CD7 expression or function link to conditions like T-cell acute lymphoblastic leukemia (T-ALL) and certain autoimmune diseases. In T-ALL abnormal CD7 expression serves as an indicator for diagnosis and disease progression monitoring. CD7 also interacts with proteins such as CD45 and CD3 which play roles in leukemogenesis and immune system dysregulation. Studies also explore anti-CD7 strategies for therapeutic interventions aiming to modulate its activity for treating these conditions effectively.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834), expandable thumbnail

    Western blot - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)

    This data was developed using Anti-CD7 antibody [EPR4242] ab109296, the same antibody clone in a different buffer formulation.

    The molecular mass observed is consistent with what has been described in the literatures (PMID: 24157461, 2479685).

    All lanes: Western blot - Anti-CD7 antibody [EPR4242] (Anti-CD7 antibody [EPR4242] ab109296) at 1/10000 dilution

    All lanes: Human Spleen Lysate at 15 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 25 kDa

    Observed band size: 37 kDa

  • Flow Cytometry (Intracellular) - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)

    This data was developed using Anti-CD7 antibody [EPR4242] ab109296, the same antibody clone in a different buffer formulation.

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte) cells labelling CD7 with purifiedab109296 at 1/100 dilution (1�g) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor� 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834), expandable thumbnail

    Immunoprecipitation - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)

    This data was developed using Anti-CD7 antibody [EPR4242] ab109296, the same antibody clone in a different buffer formulation.

    CD7 was immunoprecipitated from 0.35 mg Human spleen lysate 10 ug with purified Anti-CD7 antibody [EPR4242] ab109296 at 1/50. Western blot was performed on the immunoprecipitate using Anti-CD7 antibody [EPR4242] ab109296 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Human spleen lysate 10 ug

    Lane 2: Anti-CD7 antibody [EPR4242] ab109296 IP in Human spleen lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD7 antibody [EPR4242] ab109296 in Human spleen lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

    All lanes: Immunoprecipitation - Anti-CD7 antibody [EPR4242] (Anti-CD7 antibody [EPR4242] ab109296)

    Predicted band size: 25 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)

    This data was developed using Anti-CD7 antibody [EPR4242] ab109296, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling CD7 with Purified Anti-CD7 antibody [EPR4242] ab109296 at 1/50 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • OI-RD Scanning - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834), expandable thumbnail

    OI-RD Scanning - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)

    Tissue Microarrays stained for "Anti-CD7 antibody [EPR4242]" using "Anti-CD7 antibody [EPR4242] ab109296"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with Anti-CD7 antibody [EPR4242] ab109296 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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