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Rabbit Recombinant Monoclonal CD7 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (AB230904), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (AB230904), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (AB230904), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (AB230904), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (AB230904), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

Antigen Retrieval: Boil tissue section in 1 mM EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Associated Products

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Target data

Function

Transmembrane glycoprotein expressed by T-cells and natural killer (NK) cells and their precursors (PubMed:7506726). Plays a costimulatory role in T-cell activation upon binding to its ligand K12/SECTM1 (PubMed:10652336). In turn, mediates the production of cytokines such as IL-2 (PubMed:1709867). On resting NK-cells, CD7 activation results in a significant induction of interferon-gamma levels (PubMed:7506726).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal CD7 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

SP94

Purification technique

Affinity purification Protein A/G

Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab230904 is the carrier-free version of Anti-CD7 antibody [SP94] ab183325.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD7 also known as Leu-9 or GP40 is a cell surface protein with a mass of approximately 40 kDa. The target is mainly expressed on T cells and NK cells and can be found during early T-cell development. Researchers also report CD7 expression in some B cell lineage progenitors. The CD7 function involves mediating cell signal transduction which contributes to the activation and differentiation of immune cells making it an important target in immunology studies.

Biological function summary

CD7 plays a role in modulating interactions between immune cells without being part of a larger complex. It bridges interactions promoting immune responses by influencing T cell proliferation and cytokine production. CD7 control is important in regulating immune response intensity and provides an essential checkpoint in immune system modulation. Variations in CD7 function can significantly influence immune cell behavior impacting host defense mechanisms.

Pathways

CD7 participates in immune signaling pathways such as the T-cell receptor (TCR) signaling pathway. This pathway is essential for T cell activation and involves proteins like CD3 and ZAP-70 which work together with CD7 to transduce activation signals. Alterations in the CD7 pathway can affect the downstream signaling events leading to changes in immune cell activation and function.

Associated diseases and disorders

Aberrations in CD7 expression or function link to conditions like T-cell acute lymphoblastic leukemia (T-ALL) and certain autoimmune diseases. In T-ALL abnormal CD7 expression serves as an indicator for diagnosis and disease progression monitoring. CD7 also interacts with proteins such as CD45 and CD3 which play roles in leukemogenesis and immune system dysregulation. Studies also explore anti-CD7 strategies for therapeutic interventions aiming to modulate its activity for treating these conditions effectively.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach carcinoma tissue sections labeling CD7 with Anti-CD7 antibody [SP94] ab183325 at 1: 50 dilution (2.0 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the stromal cells in human stomach carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with Anti-CD7 antibody [SP94] ab183325 for 30 mins at room temperature. This image was generated using Anti-CD7 antibody [SP94] ab183325, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD7 with Anti-CD7 antibody [SP94] ab183325 at 1: 50 dilution (2.0 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with Anti-CD7 antibody [SP94] ab183325 for 30 mins at room temperature. This image was generated using Anti-CD7 antibody [SP94] ab183325, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904)

    Immunohistochemical analysis of paraffin embedded Human spleen tissue labeling CD7 with Anti-CD7 antibody [SP94] ab183325 at 1/50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD7 antibody [SP94] ab183325).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904)

    Immunohistochemical analysis of paraffin embedded Human stomach adenocarcinoma tissue labeling CD7 with Anti-CD7 antibody [SP94] ab183325 at 1/50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD7 antibody [SP94] ab183325).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904)

    Immunohistochemical analysis of paraffin embedded Human stomach tissue labeling CD7 with Anti-CD7 antibody [SP94] ab183325 at 1/50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD7 antibody [SP94] ab183325).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904)

    Immunohistochemical analysis of paraffin embedded Human renal cell carcinoma tissue labeling CD7 with Anti-CD7 antibody [SP94] ab183325 at 1/50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD7 antibody [SP94] ab183325).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904)

    Immunohistochemical analysis of paraffin embedded Human skin squamous cell carcinoma tissue labeling CD7 with Anti-CD7 antibody [SP94] ab183325 at 1/50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD7 antibody [SP94] ab183325).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904)

    Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling CD7 with Anti-CD7 antibody [SP94] ab183325 at 1/50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD7 antibody [SP94] ab183325).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904)

    Immunohistochemical analysis of paraffin embedded Human reactive lymph node tissue labeling CD7 with Anti-CD7 antibody [SP94] ab183325 at 1/50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD7 antibody [SP94] ab183325).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904)

    Immunohistochemical analysis of paraffin embedded Human B cell lymphoma tissue labeling CD7 with Anti-CD7 antibody [SP94] ab183325 at 1/50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD7 antibody [SP94] ab183325).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904)

    Immunohistochemical analysis of paraffin embedded Human thymus tissue labeling CD7 with Anti-CD7 antibody [SP94] ab183325 at 1/50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD7 antibody [SP94] ab183325).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904)

    Paraffin-embedded human colon tissue stained for CD7 with Anti-CD7 antibody [SP94] ab183325 at a 1/50 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD7 antibody [SP94] ab183325).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904)

    Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling CD7 with Anti-CD7 antibody [SP94] ab183325 at 1/50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD7 antibody [SP94] ab183325).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [SP94] - BSA and Azide free (ab230904)

    Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling CD7 with Anti-CD7 antibody [SP94] ab183325 at 1/50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD7 antibody [SP94] ab183325).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com