Rabbit Polyclonal CD70 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CD70.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CD70. The exact immunogen used to generate this antibody is proprietary information. Database link P32970
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Common marmoset | Predicted |
| Gorilla | Predicted |
| Monkey | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5.00000-10.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey, Gorilla, Common marmoset | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells (PubMed:28011863, PubMed:28011864, PubMed:8387892). The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells (PubMed:28011863, PubMed:28011864).
Alternative names
CD70, CD27L, CD27LG, TNFSF7, CD70 antigen, CD27 ligand, Tumor necrosis factor ligand superfamily member 7, CD27-L
Recommended products
Rabbit Polyclonal CD70 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CD70.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CD70. The exact immunogen used to generate this antibody is proprietary information. Database link P32970
- Purification technique
- Affinity purification Immunogen
- Specificity
BLAST analysis of the peptide immunogen showed no homology with other Human proteins.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD70 also known as CD27 ligand or CD27L is a type II transmembrane protein with a molecular mass of approximately 30 kDa. It belongs to the tumor necrosis factor (TNF) superfamily and is encoded by the CD70 gene. The expression of CD70 occurs mainly on activated T and B cells dendritic cells and some NK cells but not on resting lymphocytes. In addition CD70 expression is observed in certain malignancies and inflammatory conditions.
- Biological function summary
CD70 plays an essential role in the regulation of immune responses. It does this by binding to its receptor CD27 forming a receptor-ligand complex that triggers signaling pathways vital for T cell proliferation survival and differentiation. This interaction also influences the production of immunoglobulins by B cells. CD70-CD27 signaling is particularly important in shaping adaptive immunity enhancing the body's ability to respond to pathogens effectively.
- Pathways
CD70 is integral in the activation of the NF-κB and MAPK pathways. These pathways are activated upon the interaction of CD70 with CD27 promoting cell survival proliferation and pro-inflammatory cytokine production. Other proteins related to CD70 through these pathways include TRAF2 and TRAF3 which are essential for downstream signaling events that modulate immune responses.
- Associated diseases and disorders
CD70 has been implicated in autoimmune diseases and certain cancers. For instance it plays a role in the pathogenesis of lupus by contributing to abnormal immune activation. Overexpression of CD70 is also associated with various cancers such as renal cell carcinoma where it can support tumor cell growth and immune evasion. Through these conditions CD70 interacts with proteins like BCL-2 in cancer affecting cell survival pathways while its interaction with CD27 influences the immune deregulation seen in lupus.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD70 antibody (ab133398)
Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling CD70 with ab133398 antibody at 10μg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD70 antibody (ab133398)
Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling CD70 with ab133398 antibody at 10μg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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