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Rabbit Monoclonal CD70 antibody. Carrier free. Suitable for IHC-P, WB, IP, ICC/IF and reacts with Human samples.

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Images

Western blot - Anti-CD70 antibody [EPR26536-122] (BSA and Azide free) (AB300084), expandable thumbnail
  • Western blot - Anti-CD70 antibody [EPR26536-122] (BSA and Azide free) (AB300084), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD70 antibody [EPR26536-122] (BSA and Azide free) (AB300084), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD70 antibody [EPR26536-122] (BSA and Azide free) (AB300084), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD70 antibody [EPR26536-122] (BSA and Azide free) (AB300084), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWBIPICC/IF
Human
Tested
Not recommended
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells (PubMed:28011863, PubMed:28011864, PubMed:8387892). The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells (PubMed:28011863, PubMed:28011864).

Alternative names

CD70, CD27L, CD27LG, TNFSF7, CD70 antigen, CD27 ligand, Tumor necrosis factor ligand superfamily member 7, CD27-L

Recommended products

Rabbit Monoclonal CD70 antibody. Carrier free. Suitable for IHC-P, WB, IP, ICC/IF and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26536-122
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot

Notes

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD70 also known as CD27 ligand or CD27L is a type II transmembrane protein with a molecular mass of approximately 30 kDa. It belongs to the tumor necrosis factor (TNF) superfamily and is encoded by the CD70 gene. The expression of CD70 occurs mainly on activated T and B cells dendritic cells and some NK cells but not on resting lymphocytes. In addition CD70 expression is observed in certain malignancies and inflammatory conditions.

Biological function summary

CD70 plays an essential role in the regulation of immune responses. It does this by binding to its receptor CD27 forming a receptor-ligand complex that triggers signaling pathways vital for T cell proliferation survival and differentiation. This interaction also influences the production of immunoglobulins by B cells. CD70-CD27 signaling is particularly important in shaping adaptive immunity enhancing the body's ability to respond to pathogens effectively.

Pathways

CD70 is integral in the activation of the NF-κB and MAPK pathways. These pathways are activated upon the interaction of CD70 with CD27 promoting cell survival proliferation and pro-inflammatory cytokine production. Other proteins related to CD70 through these pathways include TRAF2 and TRAF3 which are essential for downstream signaling events that modulate immune responses.

Associated diseases and disorders

CD70 has been implicated in autoimmune diseases and certain cancers. For instance it plays a role in the pathogenesis of lupus by contributing to abnormal immune activation. Overexpression of CD70 is also associated with various cancers such as renal cell carcinoma where it can support tumor cell growth and immune evasion. Through these conditions CD70 interacts with proteins like BCL-2 in cancer affecting cell survival pathways while its interaction with CD27 influences the immune deregulation seen in lupus.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-CD70 antibody [EPR26536-122] (BSA and Azide free) (ab300084), expandable thumbnail

    Western blot - Anti-CD70 antibody [EPR26536-122] (BSA and Azide free) (ab300084)

    This data was developed using Anti-CD70 antibody [EPR26536-122] ab300083, the same antibody clone in a different buffer formulation.

    5% NFDM/TBST was used as blosking and diluting buffer.

    Samples are non-boiled as boiling may cause protein aggregates.
    The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 25691774, 11980654).
    Low expression: Jurkat, SH-SY5Y ( PMID: 16892042).

    Exposure time:

    Lane1: 48 seconds;
    Lane2: 10 seconds

    All lanes: Western blot - Anti-CD70 antibody [EPR26536-122] (Anti-CD70 antibody [EPR26536-122] ab300083) at 1/1000 dilution

    Lane 1: U266B1 (human multiple myeloma B lymphocyte) whole cell lysate at 20 µg

    Lane 2: SK-OV-3 (human ovarian cancer epithelial cell) whole cell lysate at 20 µg

    Lane 3: A-498 (Human kidney carcinoma) whole cell lysate at 20 µg

    Lane 4: 786-O (Human kidney renal cell adenocarcinoma) whole cell lysate at 20 µg

    Lane 5: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

    Lane 6: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/5000 dilution

    Predicted band size: 21 kDa

    Observed band size: 27 kDa, 54 kDa, 81 kDa

  • Western blot - Anti-CD70 antibody [EPR26536-122] (BSA and Azide free) (ab300084), expandable thumbnail

    Western blot - Anti-CD70 antibody [EPR26536-122] (BSA and Azide free) (ab300084)

    This data was developed using Anti-CD70 antibody [EPR26536-122] ab300083, the same antibody clone in a different buffer formulation.

    5% NFDM/TBST was used as blocking and diluting buffer.

    This blot was developed using a higher sensitivity ECL substrate.
    Samples are non-boiled as boiling may cause protein aggregates.
    The expression profile/molecular weight observed is consistent with that described in the literature (PMID: 25691774, 11980654).

    All lanes: Western blot - Anti-CD70 antibody [EPR26536-122] (Anti-CD70 antibody [EPR26536-122] ab300083) at 1/1000 dilution

    All lanes: Human lymphoma tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/5000 dilution

    Predicted band size: 21 kDa

    Observed band size: 27 kDa, 54 kDa, 81 kDa

    Exposure time: 81s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD70 antibody [EPR26536-122] (BSA and Azide free) (ab300084), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD70 antibody [EPR26536-122] (BSA and Azide free) (ab300084)

    This data was developed using Anti-CD70 antibody [EPR26536-122] ab300083, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human NKT lymphoma tissue labeling CD70 with Anti-CD70 antibody [EPR26536-122] ab300083 at 1/500 dilution (0.868 μg/ml), followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining in human NKT lymphoma is observed. The section was incubated with Anti-CD70 antibody [EPR26536-122] ab300083 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD70 antibody [EPR26536-122] (BSA and Azide free) (ab300084), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD70 antibody [EPR26536-122] (BSA and Azide free) (ab300084)

    This data was developed using Anti-CD70 antibody [EPR26536-122] ab300083, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human lymph node tissue labeling CD70 with Anti-CD70 antibody [EPR26536-122] ab300083 at 1/500 dilution (0.868 μg/ml), followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining in human lymph node is observed. The section was incubated with Anti-CD70 antibody [EPR26536-122] ab300083 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD70 antibody [EPR26536-122] (BSA and Azide free) (ab300084), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD70 antibody [EPR26536-122] (BSA and Azide free) (ab300084)

    This data was developed using Anti-CD70 antibody [EPR26536-122] ab300083, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded clear cell carcinoma tissue labeling CD70 with Anti-CD70 antibody [EPR26536-122] ab300083 at 1/500 dilution (0.868 μg/ml), followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining in clear cell carcinoma of human kidney is observed. The section was incubated with Anti-CD70 antibody [EPR26536-122] ab300083 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

  • Immunoprecipitation - Anti-CD70 antibody [EPR26536-122] (BSA and Azide free) (ab300084), expandable thumbnail

    Immunoprecipitation - Anti-CD70 antibody [EPR26536-122] (BSA and Azide free) (ab300084)

    This data was developed using anti CD70 antibody [EPR26536-122] the same antibody clone in a different buffer formulation.

    CD70 was immunoprecipitated from 0.35 mg A-498 (Human kidney carcinoma) whole cell lysate with anti CD70 antibody Anti-CD70 antibody [EPR26536-122] ab300083 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CD70 antibody [EPR26536-122] ab300083 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: A-498 (Human kidney carcinoma) whole cell lysate 10 μg (Inset)

    Lane 2: Anti-CD70 antibody [EPR26536-122] ab300083 IP in A-498 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD70 antibody [EPR26536-122] ab300083 in A-498 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 32 seconds

    The CD27 monomer and dimer were enriched by IP.

    All lanes: Immunoprecipitation - Anti-CD70 antibody [EPR26536-122] (Anti-CD70 antibody [EPR26536-122] ab300083)

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD70 antibody [EPR26536-122] (BSA and Azide free) (ab300084), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD70 antibody [EPR26536-122] (BSA and Azide free) (ab300084)

    This data was developed using Anti-CD70 antibody [EPR26536-122] ab300083, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling CD70 with Anti-CD70 antibody [EPR26536-122] ab300083 at 1/500 dilution (0.868 μg/ml), followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). Positive staining in human lung cancer is observed. The section was incubated with Anti-CD70 antibody [EPR26536-122] ab300083 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD70 antibody [EPR26536-122] (BSA and Azide free) (ab300084), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD70 antibody [EPR26536-122] (BSA and Azide free) (ab300084)

    This data was developed using Anti-CD70 antibody [EPR26536-122] ab300083, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized A-498 (human kidney epithelial) cells labeling CD70 with Anti-CD70 antibody [EPR26536-122] ab300083 at 1/50 (8.68 μg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 μg/ml) dilution (Green). Confocal showing cytoplasmic and membranous staining in A-498 cell line. Negative control: Jurkat (PMID: 15226368). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/ml) dilution.

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Product protocols

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