JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB171218

Anti-CD70 antibody [FR70] - Low endotoxin, Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rat Monoclonal CD70 antibody. Carrier free. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

CD70, CD27L, CD27LG, TNFSF7, CD70 antigen, CD27 ligand, Tumor necrosis factor ligand superfamily member 7, CD27-L

1 Images
Flow Cytometry - Anti-CD70 antibody [FR70] - Low endotoxin, Azide free (AB171218)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD70 antibody [FR70] - Low endotoxin, Azide free (AB171218)

Flow cytometric analysis of 4-day LPS activated BALB/c splenocytes labeling CD70 with 0.25 μg ab171218 (filled histogram) with Rat IgG2b kappa Isotype Control (open histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE. Total viable cells were used for analysis.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

FR70

Isotype

IgG2b

Light chain type

kappa

Carrier free

Yes

Reacts with

Mouse

Applications

Flow Cyt

applications

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.5 µg for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p>A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL.</p><p>ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }
style
left-column

Product details

Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD70 also known as CD27 ligand or CD27L is a type II transmembrane protein with a molecular mass of approximately 30 kDa. It belongs to the tumor necrosis factor (TNF) superfamily and is encoded by the CD70 gene. The expression of CD70 occurs mainly on activated T and B cells dendritic cells and some NK cells but not on resting lymphocytes. In addition CD70 expression is observed in certain malignancies and inflammatory conditions.
Biological function summary

CD70 plays an essential role in the regulation of immune responses. It does this by binding to its receptor CD27 forming a receptor-ligand complex that triggers signaling pathways vital for T cell proliferation survival and differentiation. This interaction also influences the production of immunoglobulins by B cells. CD70-CD27 signaling is particularly important in shaping adaptive immunity enhancing the body's ability to respond to pathogens effectively.

Pathways

CD70 is integral in the activation of the NF-κB and MAPK pathways. These pathways are activated upon the interaction of CD70 with CD27 promoting cell survival proliferation and pro-inflammatory cytokine production. Other proteins related to CD70 through these pathways include TRAF2 and TRAF3 which are essential for downstream signaling events that modulate immune responses.

CD70 has been implicated in autoimmune diseases and certain cancers. For instance it plays a role in the pathogenesis of lupus by contributing to abnormal immune activation. Overexpression of CD70 is also associated with various cancers such as renal cell carcinoma where it can support tumor cell growth and immune evasion. Through these conditions CD70 interacts with proteins like BCL-2 in cancer affecting cell survival pathways while its interaction with CD27 influences the immune deregulation seen in lupus.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells (PubMed : 28011863, PubMed : 28011864, PubMed : 8387892). The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells (PubMed : 28011863, PubMed : 28011864).
See full target information CD70
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of neurovirology 25:384-396 PubMed30758811

2019

Osteopontin counters human immunodeficiency virus type 1-induced impairment of neurite growth through mammalian target of rapamycin and beta-integrin signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Mathilde Calvez,George Hseeh,Simon Benzer,Amanda M Brown
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB234218

PE Anti-CD70 antibody [Ki-24]

Flow Cytometry - PE Anti-CD70 antibody [Ki-24] (AB234218)

  • 0
  • 0
Primary Antibodies

AB269573

Anti-CD70 antibody [BU69] - BSA and Azide free

BOND RX™ Validated

Immunohistochemistry (Frozen sections) - Anti-CD70 antibody [BU69] - BSA and Azide free (AB269573)

  • 0
  • 0
Primary Antibodies

AB77868

Anti-CD70 antibody [BU69]

BOND RX™ Validated|Trial Size

Immunohistochemistry (Frozen sections) - Anti-CD70 antibody [BU69] (AB77868)

  • 5
  • 4
Primary Antibodies

AB223292

Anti-CD70 antibody [EPR21946-290]

RabMAb|Recombinant|Trial Size

Flow Cytometry - Anti-CD70 antibody [EPR21946-290] (AB223292)

  • 0
  • 0
Primary Antibodies

AB239348

Anti-CD70 antibody [EPR21946-290] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-CD70 antibody [EPR21946-290] - BSA and Azide free (AB239348)

  • 0
  • 0
Primary Antibodies

AB133398

Anti-CD70 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD70 antibody (AB133398)

  • 0
  • 0
Primary Antibodies

AB108482

Anti-CD70 antibody [TAN 1-7]

  • 0
  • 0

Complementary Products

Assay Kits

AB267612

Human CD70 ELISA Kit

Sandwich ELISA - Human CD70 ELISA Kit (AB267612)

  • 0
  • 0
Primary Antibodies

AB170867

Anti-MHC Class II beta antibody [EPR11227]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC Class II beta antibody [EPR11227] (AB170867)

  • 0
  • 0
Primary Antibodies

AB224639

Anti-CD40 antibody [EPR20735]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR20735] (AB224639)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB108309

Anti-CD1a antibody [EP3622]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1a antibody [EP3622] (AB108309)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com