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Rabbit Polyclonal CD72 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human B-cell differentiation antigen CD72 aa 150-350.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD72 antibody (AB204336), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD72 antibody (AB204336), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human B-cell differentiation antigen CD72 aa 150-350. The exact immunogen used to generate this antibody is proprietary information. Database link P21854

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IF
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/1000.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

0.25000-2.00000 µg/mL

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Co-receptor of B cell receptor (BCR) that plays both positive and negative roles on B-cell functions. Recognizes the Sm/ribonucleoprotein (RNP) self-antigen ligand, and coligation of CD72 and BCR inhibits BCR signaling. Mechanistically, ligand binding leads to the recruitment of PTPN6/SHP-1 to the BCR complex which is inhibitory to BCR signaling. Acts also as a ligand for CD5 and thereby plays a critical role in maintaining regulatory T and B-cell homeostasis.

Alternative names

Recommended products

Rabbit Polyclonal CD72 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human B-cell differentiation antigen CD72 aa 150-350.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human B-cell differentiation antigen CD72 aa 150-350. The exact immunogen used to generate this antibody is proprietary information. Database link P21854
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD72 also known as Lyb-2 is a membrane protein with a molecular weight of approximately 45-50 kDa. It is part of the C-type lectin family and expresses mainly on B cells. CD72 serves as a receptor and recognizes ligands to mediate cell signaling. The protein is also present in some myeloid cells contributing to its diverse roles in the immune system. The function of CD72 involves the modulation of B cell receptor (BCR) signaling which is important for B cell development and activation.

Biological function summary

CD72 functions as a regulatory protein that influences B cell activity by modulating BCR signaling thresholds. CD72 is part of receptor-ligand complexes on the B cell surface. It operates alongside other proteins like CD5 and SHP-1 to fine-tune immune responses. By interacting with these proteins CD72 helps in maintaining immune balance and prevents overactivation that can lead to autoimmune reactions. This protein modulates both positive and negative signaling affecting B cell survival and differentiation.

Pathways

CD72 influences immune response pathways specifically the BCR signaling pathway and the downstream PI3K-Akt pathway. As a negative regulator CD72 works in tandem with proteins such as Lyn and SHP-1 to attenuate BCR signal transduction. These signaling cascades play a role in immune responses lymphocyte development and adaptation. The involvement of CD72 in these pathways illustrates its function in balancing the intensity of signals received by B cells ultimately affecting immune response outcomes.

Associated diseases and disorders

CD72 dysregulation has been linked to systemic lupus erythematosus (SLE) and certain lymphomas. Abnormal expression or function of CD72 can lead to improper B cell activation contributing to autoimmune conditions like SLE. In these diseases CD72 interaction with proteins such as SHP-1 and CD22 becomes disrupted affecting immune tolerance and promoting pathological immune responses. Additionally altered CD72 expression or mutations have been observed in B cell malignancies indicating its role in the pathology of these disorders.

Product promise

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Full details and terms and conditions can be found here:
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2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD72 antibody (ab204336), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD72 antibody (ab204336)

    Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling CD72 with ab204336 at 1/500 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD72 antibody (ab204336), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD72 antibody (ab204336)

    SH-SY5Y cells stained for CD72 (green) using ab204336 at 2 μg/ml in ICC/IF.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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