Rabbit Recombinant Monoclonal CD72 antibody. Carrier free. Suitable for Flow Cyt, IHC-P, mIHC and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (AB324360), expandable thumbnail](https://content.abcam.com/products/images/cd72-antibody-epr29739-578-bsa-and-azide-free-ab324360--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img447305.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (AB324360)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (AB324360), expandable thumbnail](https://content.abcam.com/products/images/cd72-antibody-epr29739-578-bsa-and-azide-free-ab324360--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img447304.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (AB324360)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (AB324360), expandable thumbnail](https://content.abcam.com/products/images/cd72-antibody-epr29739-578-bsa-and-azide-free-ab324360--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img447303.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (AB324360)")
![Multiplex immunohistochemistry - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (AB324360), expandable thumbnail](https://content.abcam.com/products/images/cd72-antibody-epr29739-578-bsa-and-azide-free-ab324360--multiplex-immunohistochemistry-img447317.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (AB324360)")
![Flow Cytometry - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (AB324360), expandable thumbnail](https://content.abcam.com/products/images/cd72-antibody-epr29739-578-bsa-and-azide-free-ab324360--flow-cytometry-img447313.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (AB324360)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | IHC-P | mIHC | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Co-receptor of B cell receptor (BCR) that plays both positive and negative roles on B-cell functions. Recognizes the Sm/ribonucleoprotein (RNP) self-antigen ligand, and coligation of CD72 and BCR inhibits BCR signaling. Mechanistically, ligand binding leads to the recruitment of PTPN6/SHP-1 to the BCR complex which is inhibitory to BCR signaling. Acts also as a ligand for CD5 and thereby plays a critical role in maintaining regulatory T and B-cell homeostasis.
Alternative names
CD72, B-cell differentiation antigen CD72, Lyb-2
Rabbit Recombinant Monoclonal CD72 antibody. Carrier free. Suitable for Flow Cyt, IHC-P, mIHC and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR29739-578
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab324360 is the carrier-free version of Anti-CD72 antibody [EPR29739-578] ab324351
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (ab324360), expandable thumbnail](https://content.abcam.com/products/images/cd72-antibody-epr29739-578-bsa-and-azide-free-ab324360--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img447305.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (ab324360)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (ab324360)
CD72 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human kidney using rabbit Anti-CD72 antibody
This data was developed using Anti-CD72 antibody [EPR29739-578] ab324351, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CD72 with Anti-CD72 antibody [EPR29739-578] ab324351 at 1/500 (1.018 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Negative control: Weak staining on human kidney.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (ab324360), expandable thumbnail](https://content.abcam.com/products/images/cd72-antibody-epr29739-578-bsa-and-azide-free-ab324360--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img447304.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (ab324360)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (ab324360)
CD72 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human B-Cell follicular lymphoma using rabbit Anti-CD72 antibody
This data was developed using Anti-CD72 antibody [EPR29739-578] ab324351, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human B-Cell follicular lymphoma tissue labeling CD72 with Anti-CD72 antibody [EPR29739-578] ab324351 at 1/500 (1.018 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on human B-Cell follicular lymphoma.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (ab324360), expandable thumbnail](https://content.abcam.com/products/images/cd72-antibody-epr29739-578-bsa-and-azide-free-ab324360--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img447303.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (ab324360)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (ab324360)
CD72 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human tonsil using rabbit Anti-CD72 antibody
This data was developed using Anti-CD72 antibody [EPR29739-578] ab324351, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD72 with Anti-CD72 antibody [EPR29739-578] ab324351 at 1/500 (1.018 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on human tonsil.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Multiplex immunohistochemistry - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (ab324360), expandable thumbnail](https://content.abcam.com/products/images/cd72-antibody-epr29739-578-bsa-and-azide-free-ab324360--multiplex-immunohistochemistry-img447317.jpg "Multiplex immunohistochemistry - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (ab324360)")
Multiplex immunohistochemistry - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (ab324360)
CD72 Multiplex immunohistochemistry staining of Human Hodgkin's lymphoma using rabbit Anti-CD72 antibody
This data was developed using Anti-CD72 antibody [EPR29739-578] ab324351, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human hodgkin's lymphoma tissue staining CD72 with Anti-CD72 antibody [EPR29739-578] ab324351 at a 1:500 (1.018 ug/ml) dilution, Anti-CD19 antibody [SP291] - C-terminal ab227688 anti-CD19 used at 1:100 (0.15 ug/ml) dilution and Anti-CD3 epsilon antibody [CAL57] ab237721 anti-CD3 used at a 1:2000 (0.26 ug/ml) dilution.
Panel A: merged staining of anti-CD72 (green; Opal™520), anti-CD19 (magenta; Opal™570) and anti-CD3 (yellow; Opal™690) on human Hodgkin's lymphoma.
Panel B: anti-CD72 staining immune cells in human Hodgkin's lymphoma.
Panel C: ant-CD19 staining B lymphocytes in human Hodgkin's lymphoma.
Panel D: ant-CD3 staining T lymphocytes in human Hodgkin's lymphoma.
Nuclear DNA was labeled with DAPI (shown in blue).The section was incubated in three rounds of staining: in the order of Anti-CD72 antibody [EPR29739-578] ab324351, Anti-CD19 antibody [SP291] - C-terminal ab227688 and Anti-CD3 epsilon antibody [CAL57] ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Nuclear counter stain with DAPI.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Flow Cytometry - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (ab324360), expandable thumbnail](https://content.abcam.com/products/images/cd72-antibody-epr29739-578-bsa-and-azide-free-ab324360--flow-cytometry-img447313.jpg "Flow Cytometry - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (ab324360)")
Flow Cytometry - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (ab324360)
CD72 Flow Cytometry staining of Human PBMC (human peripheral blood mononuclear cell) using rabbit Anti-CD72 antibody
This data was developed using Anti-CD72 antibody [EPR29739-578] ab324351, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD72 with Anti-CD72 antibody [EPR29739-578] ab324351 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Gated on viable cells.
Cells are co-stained with CD19 conjugated to Alexa Fluor® 647.![Flow Cytometry - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (ab324360), expandable thumbnail](https://content.abcam.com/products/images/cd72-antibody-epr29739-578-bsa-and-azide-free-ab324360--flow-cytometry-img447314.jpg "Flow Cytometry - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (ab324360)")
Flow Cytometry - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (ab324360)
CD72 Flow Cytometry staining of Human PBMC (human peripheral blood mononuclear cell) using rabbit Anti-CD72 antibody
This data was developed using Anti-CD72 antibody [EPR29739-578] ab324351, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD72 with Anti-CD72 antibody [EPR29739-578] ab324351 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Gated on viable cells.
Cells are co-stained with CD3 conjugated to Brilliant Violet 421.
![Flow Cytometry - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (ab324360), expandable thumbnail](https://content.abcam.com/products/images/cd72-antibody-epr29739-578-bsa-and-azide-free-ab324360--flow-cytometry-img447314.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD72 antibody [EPR29739-578] - BSA and Azide free (ab324360)")
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