Mouse Monoclonal CD73 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NT5E aa 1-250.
Images
![Flow Cytometry - Anti-CD73 antibody [4G6E3] (AB202122), expandable thumbnail](https://content.abcam.com/products/images/cd73-antibody-4g6e3-ab202122--flow-cytometry-img121405.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD73 antibody [4G6E3] (AB202122)")
![Western blot - Anti-CD73 antibody [4G6E3] (AB202122), expandable thumbnail](https://content.abcam.com/products/images/cd73-antibody-4g6e3-ab202122--western-blot-img172695.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD73 antibody [4G6E3] (AB202122)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [4G6E3] (AB202122), expandable thumbnail](https://content.abcam.com/products/images/cd73-antibody-4g6e3-ab202122--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132330.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [4G6E3] (AB202122)")
![Western blot - Anti-CD73 antibody [4G6E3] (AB202122), expandable thumbnail](https://content.abcam.com/products/images/cd73-antibody-4g6e3-ab202122--western-blot-img2458.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD73 antibody [4G6E3] (AB202122)")
![Western blot - Anti-CD73 antibody [4G6E3] (AB202122), expandable thumbnail](https://content.abcam.com/products/images/cd73-antibody-4g6e3-ab202122--western-blot-img64643.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD73 antibody [4G6E3] (AB202122)")
Publications
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- International journal of molecular sciences 24:2023BMSC-Derived Exosomal CircHIPK3 Promotes Osteogenic Differentiation of MC3T3-E1 Cells via Mitophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShaoyang Ma,Sijia Li,Yuchen Zhang,Jiaming Nie,Jiao Cao,Ang Li,Ye Li,Dandan PeiPubMed 36769123
- Cellular & molecular biology letters 27:862022Anti-aging effect of β-carotene through regulating the KAT7-P15 signaling axis, inflammation and oxidative stress process.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei V Zheng,Wang Xu,Yaqin Li,Jie Qin,Tao Zhou,Dezhi Li,Yanwei Xu,Xianyi Cheng,Yu Xiong,Zaizhong ChenPubMed 36209059
- Disease markers 2022:69436302022Kartogenin Induced Adipose-Derived Stem Cell Exosomes Enhance the Chondrogenic Differentiation Ability of Adipose-Derived Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAiguo Xie,Jixin Xue,Yuting Wang,Chao Yang,Miao Xu,Yongkang JiangPubMed 36072901
- Analytical cellular pathology (Amsterdam) 2022:93024962022Extracellular Vesicle-Encapsulated MicroRNA-375 from Bone Marrow-Derived Mesenchymal Stem Cells Inhibits Hepatocellular Carcinoma Progression through Regulating HOXB3-Mediated Wnt/-Catenin Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhaoxia Yu,Ju Liu,Qiqi Fan,Jun Yu,Xiaoting Ren,Xiaobin WangPubMed 35127344
- Journal of tissue engineering and regenerative medicine 16:311-3292022LncRNA metastasis-associated lung adenocarcinoma transcript-1 promotes osteogenic differentiation of bone marrow stem cells and inhibits osteoclastic differentiation of Mø in osteoporosis via the miR-124-3p/IGF2BP1/Wnt/β-catenin axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangxin LiPubMed 34962086
- Bioengineered 12:9739-97522021A novel circular RNA circ_HN1/miR-628-5p/Ecto-5'-nucleotidase competing endogenous RNA network regulates gastric cancer development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianmin Zhang,Fang Wang,Haihui Zhang,Mingbo CaoPubMed 34637682
- International journal of stem cells 14:434-4462021MiR-148a-3p Regulates the Invasion and Odontoblastic Differentiation of Human Dental Pulp Stem Cells via the Wnt1/-Catenin Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiong Li,Lei HuangPubMed 34456188
- Experimental and therapeutic medicine 22:10912021Osteogenic ability using porous hydroxyapatite scaffold-based delivery of human placenta-derived mesenchymal stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaohua Ren,Qingwei Wang,Chunhui Liu,Qian Zhao,Jiajun Zheng,Kun Tian,Huijuan Xu,Yandong MuPubMed 34504545
- Journal of tissue engineering and regenerative medicine 15:699-7112021The function and mechanism of the miR-210-3p/KRAS axis in bone marrow-derived mesenchymal stem cell from patients with osteoporosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinghua Hu,Xi Zhu,Heng Yuan,Haifeng Li,Hongchun Liao,Shijie ChenPubMed 33982450
- Experimental and therapeutic medicine 20:1122020Pretreatment with human urine-derived stem cells protects neurological function in rats following cardiopulmonary resuscitation after cardiac arrest.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun Pan et. al.PubMed 32989390
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human NT5E aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P21589
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Reactivity data
Select an application| Flow Cyt | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/400.00000 | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
14 products for Alternative Product
Target data
Function
Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084).
Alternative names
CD73, NT5, NTE, NT5E, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, Thymidylate 5'-phosphatase
Recommended products
Mouse Monoclonal CD73 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NT5E aa 1-250.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human NT5E aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P21589
- Clone number
- 4G6E3
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purified from tissue culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to supernatant. Lot no's high than GR287343-13 are from Tissue Culture Supernatant
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD73 also known as ecto-5'-nucleotidase is an enzyme that functions at the cellular level. The CD73 protein hydrolyzes extracellular nucleotides into nucleosides by removing phosphate groups. It is a 70 kDa glycosylphosphatidylinositol-anchored molecule found on the surface of various cell types including endothelial cells lymphocytes and epithelial cells. Its presence is noted in a wide range of tissues across the human body highlighting its versatile role in cellular communication and metabolism.
- Biological function summary
CD73 participates in the regulation of purinergic signaling through adenosine production. It acts in cooperation with other cell surface enzymes such as CD39 forming a functional complex that handles ATP breakdown to adenosine. This enzymatic activity modulates immune responses tissue protection and inflammation control. In addition to immune-related functions CD73 supports the maintenance of vascular integrity and proper cellular adhesion which is important in different physiological and pathological contexts.
- Pathways
CD73 plays an important part in the adenosinergic pathway governing the conversion of AMP into adenosine. This activity impacts the signaling and function of the adenosine receptors on immune and non-immune cells. Another important pathway is the angiogenesis process where CD73 contributes by affecting endothelial cell migration and blood vessel development. Relationships with key proteins such as adenosine A2 receptors help facilitate these critical pathway activities.
- Associated diseases and disorders
CD73's involvement with immunosuppression and tumor progression becomes significant. Elevated levels of CD73 are frequently observed in some cancers where it aids in creating a favorable tumor microenvironment through increased adenosine production. This situation assists tumor cells in evading immune detection. Additionally CD73 is linked to autoimmune diseases like multiple sclerosis where it contributes to modulated lymphocyte activity. Connections with proteins such as TGF-beta further illustrate its role in disease-related pathways creating potential targets for therapeutic interventions through the use of anti-CD73 antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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7 product images
![Flow Cytometry - Anti-CD73 antibody [4G6E3] (ab202122), expandable thumbnail](https://content.abcam.com/products/images/cd73-antibody-4g6e3-ab202122--flow-cytometry-img121405.jpg "Flow Cytometry - Anti-CD73 antibody [4G6E3] (ab202122)")
Flow Cytometry - Anti-CD73 antibody [4G6E3] (ab202122)
Flow cytometric analysis of A431 cells using ab202122 at 1/200 (green) and negative control (red).
![Western blot - Anti-CD73 antibody [4G6E3] (ab202122), expandable thumbnail](https://content.abcam.com/products/images/cd73-antibody-4g6e3-ab202122--western-blot-img172695.jpg "Western blot - Anti-CD73 antibody [4G6E3] (ab202122)")
Western blot - Anti-CD73 antibody [4G6E3] (ab202122)
False colour image of Western blot: Anti-CD73 antibody [4G6E3] staining at 1/500 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab202122 was shown to bind specifically to CD73. A band was observed at 65-70 kDa in wild-type A-375 cell lysates with no signal observed at this size in NT5E knockout cell line Human NT5E knockout A375 cell line ab281591 (knockout cell lysate Human NT5E knockout A375 cell lysate ab282943). A cross-reactive band is observed at 65 kDa in all samples. To generate this image, wild-type and NT5E knockout A-375 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/20000 dilution.
All lanes: Western blot - Anti-CD73 antibody [4G6E3] (ab202122) at 1/500 dilution
Lane 1: Wild-type A-375 cell lysate at 20 µg
Lane 2: NT5E knockout A-375 cell lysate at 20 µg
Lane 2: Western blot - Human NT5E knockout A375 cell line (Human NT5E knockout A375 cell line ab281591)
Lane 3: A431 cell lysate at 20 µg
Lane 4: HepG2 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 65 kDa, 65-70 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [4G6E3] (ab202122), expandable thumbnail](https://content.abcam.com/products/images/cd73-antibody-4g6e3-ab202122--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132330.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [4G6E3] (ab202122)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [4G6E3] (ab202122)
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissue labeling CD73 with ab202122 at 1/200 with DAB staining.
![Western blot - Anti-CD73 antibody [4G6E3] (ab202122), expandable thumbnail](https://content.abcam.com/products/images/cd73-antibody-4g6e3-ab202122--western-blot-img2458.jpg "Western blot - Anti-CD73 antibody [4G6E3] (ab202122)")
Western blot - Anti-CD73 antibody [4G6E3] (ab202122)
Lanes 1 - 3: Merged signal (red and green). Green - ab202122 observed at 63 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.ab202122 was shown to recognize NT5E in wild-type A-431 cells as signal was lost at the expected MW in NT5E knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and NT5E knockout samples were subjected to SDS-PAGE. ab202122 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-CD73 antibody [4G6E3] (ab202122) at 1/1000 dilution
Lane 1: Wild-type A-431 whole cell lysate at 20 µg
Lane 2: NT5E knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg
Lane 2: Western blot - Human NT5E knockout A-431 cell line (Human NT5E knockout A-431 cell line ab261895)
Lane 3: A375 (Human malignant melanoma cell line) whole cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 63 kDa
![Western blot - Anti-CD73 antibody [4G6E3] (ab202122), expandable thumbnail](https://content.abcam.com/products/images/cd73-antibody-4g6e3-ab202122--western-blot-img64643.jpg "Western blot - Anti-CD73 antibody [4G6E3] (ab202122)")
Western blot - Anti-CD73 antibody [4G6E3] (ab202122)
All lanes: Western blot - Anti-CD73 antibody [4G6E3] (ab202122) at 1/500 dilution
All lanes: A431 cell lysate
Predicted band size: 63 kDa
![Western blot - Anti-CD73 antibody [4G6E3] (ab202122), expandable thumbnail](https://content.abcam.com/products/images/cd73-antibody-4g6e3-ab202122--western-blot-img65262.jpg "Western blot - Anti-CD73 antibody [4G6E3] (ab202122)")
Western blot - Anti-CD73 antibody [4G6E3] (ab202122)
Expected MW is 26.6 kDa
All lanes: Western blot - Anti-CD73 antibody [4G6E3] (ab202122) at 1/500 dilution
All lanes: Human CD73 (AA: 30-250) recombinant protein
Predicted band size: 63 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [4G6E3] (ab202122), expandable thumbnail](https://content.abcam.com/products/images/cd73-antibody-4g6e3-ab202122--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123999.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [4G6E3] (ab202122)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [4G6E3] (ab202122)
Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling CD73 with ab202122 at 1/200 with DAB staining.
![Western blot - Anti-CD73 antibody [4G6E3] (ab202122), expandable thumbnail](https://content.abcam.com/products/images/cd73-antibody-4g6e3-ab202122--western-blot-img65262.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD73 antibody [4G6E3] (ab202122)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [4G6E3] (ab202122), expandable thumbnail](https://content.abcam.com/products/images/cd73-antibody-4g6e3-ab202122--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123999.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [4G6E3] (ab202122)")
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