AB54217

Anti-CD73 antibody [7G2]

Name: Anti-CD73 antibody [7G2] (ab54217) | Abcam
Brand: Abcam Limited
SKU: AB54217
Price: 595 USD
Availability: InStock
Rating: 3 (3 reviews)

(3 Reviews)

(32 Publications)

Mouse Monoclonal CD73 antibody. Suitable for ICC, Flow Cyt and reacts with Human samples. Cited in 32 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human NT5E.

View Alternative Names

CD73, NT5, NTE, NT5E, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, Thymidylate 5'-phosphatase

2 Images

Flow Cytometry - Anti-CD73 antibody [7G2] (AB54217)

Flow Cyt

Unknown

Flow Cytometry - Anti-CD73 antibody [7G2] (AB54217)

ab54217 staining human peripheral blood lymphocytes by FACS analysis.

Unknown

Immunocytochemistry - Anti-CD73 antibody [7G2] (AB54217)

Immunofluorescence analysis of CD73 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ab54217 at a concentration of 1 μg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with a Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate, (1 : 2000 dilution), for 45 minutes at room temperature (Panel a : Green). Nuclei (Panel b : Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI F-actin (Panel c : Red) was stained with Rhodamine Phalloidin (1 : 300 dilution). Panel d represents the merged image showing membrane localization. Panel e represents HeLa cells showing no expression of CD73. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7G2

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

Flow Cyt, ICC

applications

Immunogen

Native Full Length Protein corresponding to Human NT5E.

P21589

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100", "ICC-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg/mL", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD73 also known as ecto-5'-nucleotidase is an enzyme that functions at the cellular level. The CD73 protein hydrolyzes extracellular nucleotides into nucleosides by removing phosphate groups. It is a 70 kDa glycosylphosphatidylinositol-anchored molecule found on the surface of various cell types including endothelial cells lymphocytes and epithelial cells. Its presence is noted in a wide range of tissues across the human body highlighting its versatile role in cellular communication and metabolism.

Biological function summary

CD73 participates in the regulation of purinergic signaling through adenosine production. It acts in cooperation with other cell surface enzymes such as CD39 forming a functional complex that handles ATP breakdown to adenosine. This enzymatic activity modulates immune responses tissue protection and inflammation control. In addition to immune-related functions CD73 supports the maintenance of vascular integrity and proper cellular adhesion which is important in different physiological and pathological contexts.

Pathways

CD73 plays an important part in the adenosinergic pathway governing the conversion of AMP into adenosine. This activity impacts the signaling and function of the adenosine receptors on immune and non-immune cells. Another important pathway is the angiogenesis process where CD73 contributes by affecting endothelial cell migration and blood vessel development. Relationships with key proteins such as adenosine A2 receptors help facilitate these critical pathway activities.

CD73's involvement with immunosuppression and tumor progression becomes significant. Elevated levels of CD73 are frequently observed in some cancers where it aids in creating a favorable tumor microenvironment through increased adenosine production. This situation assists tumor cells in evading immune detection. Additionally CD73 is linked to autoimmune diseases like multiple sclerosis where it contributes to modulated lymphocyte activity. Connections with proteins such as TGF-beta further illustrate its role in disease-related pathways creating potential targets for therapeutic interventions through the use of anti-CD73 antibodies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed : 21933152, PubMed : 22997138, PubMed : 23142347, PubMed : 24887587, PubMed : 34403084). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed : 34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed : 21933152, PubMed : 22997138, PubMed : 23142347, PubMed : 24887587, PubMed : 34403084).

See full target information NT5E

style

left-column

Publications (32)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 6:476 PubMed37127734

2023

Definitive Endodermal Cells Supply an in vitro Source of Mesenchymal Stem/Stromal Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yumeng Zhang,Ye Yi,Xia Xiao,Lingling Hu,Jiaqi Xu,Dejin Zheng,Ho Cheng Koc,Un In Chan,Ya Meng,Ligong Lu,Weiwei Liu,Xiaoling Xu,Ningyi Shao,Edwin Chong Wing Cheung,Ren-He Xu,Guokai Chen

Stem cell research & therapy 14:54 PubMed36978118

2023

MiR-21-5p regulates the dynamic of mitochondria network and rejuvenates the senile phenotype of bone marrow stromal cells (BMSCs) isolated from osteoporotic SAM/P6 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mateusz Sikora,Agnieszka Śmieszek,Ariadna Pielok,Krzysztof Marycz

Folia morphologica 83:72-82 PubMed36967622

2023

Biodegradable nanofiber coated human umbilical cord as nerve scaffold for sciatic nerve regeneration in albino Wistar rats.

Applications

Unspecified application

Species

Unspecified reactive species

Balasundari Ramesh,Jaikanth Chandrasekaran,Kotturathu Mammen Cherian,Adegbenro Omotuyi John Fakoya

Journal of nanobiotechnology 20:282 PubMed35710420

2022

Modulated nanowire scaffold for highly efficient differentiation of mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jose E Perez,Bashaer Bajaber,Nouf Alsharif,Aldo I Martínez-Banderas,Niketan Patel,Ainur Sharip,Enzo Di Fabrizio,Jasmeen Merzaban,Jürgen Kosel

Journal of cellular and molecular medicine 25:6634-6651 PubMed34075722

2021

Bone marrow stromal cells (BMSCs CD45 /CD44 /CD73 /CD90 ) isolated from osteoporotic mice SAM/P6 as a novel model for osteoporosis investigation.

Applications

Unspecified application

Species

Unspecified reactive species

Mateusz Sikora,Agnieszka Śmieszek,Krzysztof Marycz

Journal of experimental & clinical cancer research 39:238 PubMed33168024

2020

MiRNA-340-5p mediates the functional and infiltrative promotion of tumor-infiltrating CD8 T lymphocytes in human diffuse large B cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yangyang Xu,Zhenchuan Liu,Lixin Lv,Ping Li,Bing Xiu,Wenbin Qian,Aibin Liang

PeerJ 8:e9928 PubMed32999767

2020

Mesenchymal stem cell exosomes reverse acute lung injury through Nrf-2/ARE and NF-B signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Li,Xingqi Deng,Xiangling Ji,Xiaojun Shi,Zhiying Ying,Kan Shen,Dongwei Xu,Zhihui Cheng

Cell and tissue banking : PubMed32797333

2020

The influence of preparation and storage time on endothelial cells in Quarter-Descemet membrane endothelial keratoplasty (Quarter-DMEK) grafts in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Daniele Spinozzi,Alina Miron,Jessica Lie,Silke Oellerich,Sorcha Ni Dhubhghaill,Gerrit R J Melles

International journal of molecular medicine 46:1074-1084 PubMed32705163

2020

Activation of SIRT1 promotes cartilage differentiation and reduces apoptosis of nucleus pulposus mesenchymal stem cells via the MCP1/CCR2 axis in subjects with intervertebral disc degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Xuancheng Ou,Jinwei Ying,Xuedong Bai,Chaofeng Wang,Dike Ruan

Stem cell reviews and reports 16:585-595 PubMed32185666

2020

Energy Metabolism Analysis of Three Different Mesenchymal Stem Cell Populations of Umbilical Cord Under Normal and Pathologic Conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Eleonora Russo,Jea-Young Lee,Hung Nguyen,Simona Corrao,Rita Anzalone,Giampiero La Rocca,Cesar V Borlongan

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD73 antibody [7G2]

Flow Cytometry - Anti-CD73 antibody [7G2] (AB54217)

Immunocytochemistry - Anti-CD73 antibody [7G2] (AB54217)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (32)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise