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AB130451

Anti-CD73 antibody [AD2]

5

(1 Review)

|

(3 Publications)

Mouse Recombinant Monoclonal CD73 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CD73, NT5, NTE, NT5E, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, Thymidylate 5'-phosphatase

1 Images
Flow Cytometry - Anti-CD73 antibody [AD2] (AB130451)
  • Flow Cyt

Lab

Flow Cytometry - Anti-CD73 antibody [AD2] (AB130451)

Flow cytometric analysis of human PBMC cells labeling CD73 with ab130451 at 1/500 dilution (Right) compared with an isotype control (Left).

Cells were blocked with 20 μg/mL human IgG for 15 minutes on ice. Primary antibody incubation for 30 minutes on ice.

Goat Anti-Mouse IgG H&L (AlexaFluor® 488) (ab150113) was used as secondary antibody for 30 minutes on ice in the dark. Cells were also stained with Anti-Mouse CD19 conjugated to AlexaFluor® 647 for 30 minutes on ice in the dark. Added 7-AAD in 1X PBS and incubated for 5 minutes.

  • Carrier free

    Anti-CD73 antibody [AD2] - BSA and Azide free

  • 660 APC

    APC Anti-CD73 antibody [AD2]

  • 660 APC

    APC Anti-CD73 antibody [AD2]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD73 antibody [AD2]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD73 antibody [AD2]

  • 519 FITC

    FITC Anti-CD73 antibody [AD2]

  • 578 PE

    PE Anti-CD73 antibody [AD2]

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD73 antibody [AD2]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD73 antibody [AD2]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AD2

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

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Alternative Products

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Primary Antibodies

AB81289

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-4 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

This product has switched from a hybridoma to recombinant production method on 21st September 2020.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD73 also known as ecto-5'-nucleotidase is an enzyme that functions at the cellular level. The CD73 protein hydrolyzes extracellular nucleotides into nucleosides by removing phosphate groups. It is a 70 kDa glycosylphosphatidylinositol-anchored molecule found on the surface of various cell types including endothelial cells lymphocytes and epithelial cells. Its presence is noted in a wide range of tissues across the human body highlighting its versatile role in cellular communication and metabolism.
Biological function summary

CD73 participates in the regulation of purinergic signaling through adenosine production. It acts in cooperation with other cell surface enzymes such as CD39 forming a functional complex that handles ATP breakdown to adenosine. This enzymatic activity modulates immune responses tissue protection and inflammation control. In addition to immune-related functions CD73 supports the maintenance of vascular integrity and proper cellular adhesion which is important in different physiological and pathological contexts.

Pathways

CD73 plays an important part in the adenosinergic pathway governing the conversion of AMP into adenosine. This activity impacts the signaling and function of the adenosine receptors on immune and non-immune cells. Another important pathway is the angiogenesis process where CD73 contributes by affecting endothelial cell migration and blood vessel development. Relationships with key proteins such as adenosine A2 receptors help facilitate these critical pathway activities.

CD73's involvement with immunosuppression and tumor progression becomes significant. Elevated levels of CD73 are frequently observed in some cancers where it aids in creating a favorable tumor microenvironment through increased adenosine production. This situation assists tumor cells in evading immune detection. Additionally CD73 is linked to autoimmune diseases like multiple sclerosis where it contributes to modulated lymphocyte activity. Connections with proteins such as TGF-beta further illustrate its role in disease-related pathways creating potential targets for therapeutic interventions through the use of anti-CD73 antibodies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed : 21933152, PubMed : 22997138, PubMed : 23142347, PubMed : 24887587, PubMed : 34403084). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed : 34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed : 21933152, PubMed : 22997138, PubMed : 23142347, PubMed : 24887587, PubMed : 34403084).
See full target information NT5E
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 5:101572 PubMed38754420

2024

CD37 is a safe chimeric antigen receptor target to treat acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin Caulier,Sandy Joaquina,Pascal Gelebart,Tara Helén Dowling,Fatemeh Kaveh,Moritz Thomas,Luka Tandaric,Patrik Wernhoff,Niveditha Umesh Katyayini,Cara Wogsland,May Eriksen Gjerstad,Yngvar Fløisand,Gunnar Kvalheim,Carsten Marr,Sebastian Kobold,Jorrit M Enserink,Bjørn Tore Gjertsen,Emmet McCormack,Else Marit Inderberg,Sébastien Wälchli

Heliyon 7:e07996 PubMed34589625

2021

Mesenchymal stem cells act as stimulators of neurogenesis and synaptic function in a rat model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Maryam Doshmanziari,Sadegh Shirian,Mohammad-Reza Kouchakian,Seyedeh Farzaneh Moniri,Saranaz Jangnoo,Niloofar Mohammadi,Fariba Zafari

Journal of immunology (Baltimore, Md. : 1950) 162:6238-46 PubMed10229870

1999

CD38 stimulation lowers the activation threshold and enhances the alloreactivity of cord blood T cells by activating the phosphatidylinositol 3-kinase pathway and inducing CD73 expression.

Applications

Unspecified application

Species

Unspecified reactive species

P Borrione,S Peola,S Mariani,B Besostri,R Mallone,F Malavasi,A Pileri,M Massaia
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com