AB314071

Anti-CD73 antibody [BLR054F] - BSA free

Recombinant
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Rabbit Recombinant Monoclonal CD73 antibody. Suitable for ICC, IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NT5E aa 1 to C-terminus.

View Alternative Names

CD73, NT5, NTE, NT5E, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, Thymidylate 5'-phosphatase

4 Images

Supplier Data

Immunocytochemistry - Anti-CD73 antibody [BLR054F] - BSA free (AB314071)

Detection of human CD73 by immunocytochemistry. Sample : FFPE section of IMR-90 cells. Antibody : Rabbit anti-CD73 recombinant monoclonal antibody [BLR054F] (A700-054) used at 1 : 100. Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [BLR054F] - BSA free (AB314071)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [BLR054F] - BSA free (AB314071)

Detection of human CD73 by immunohistochemistry. Sample : FFPE section of human head and neck squamous cell carcinoma. Antibody : Rabbit anti-CD73 recombinant monoclonal antibody [BLR054F] (A700-054) used at 1 : 500. Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [BLR054F] - BSA free (AB314071)

Detection of human CD73 (red) by immunohistochemistry. Sample : FFPE section of human tonsil. Antibody : Rabbit anti-CD73 recombinant monoclonal antibody [BLR054F] (A700-054) used at 1 : 250. Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : Opal™. Counterstain : DAPI (blue).

Supplier Data

Western blot - Anti-CD73 antibody [BLR054F] - BSA free (AB314071)

Samples : Whole cell lysate (50 µg) from NCI-H226, U2OS, OVCAR-8, Jurkat, IMR-90, LNCaP, Hep-G2, K-562, and GaMG cells prepared using NETN lysis buffer. Antibody : Rabbit antiCD73 recombinant monoclonal antibody [BLR054F] (A700-054) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel : Rabbit anti-Actin recombinant monoclonal [BLR054F]

All lanes:

Western blot - Anti-CD73 antibody [BLR054F] - BSA free (ab314071) at 1/1000 dilution

Lane 1:

NCI-H226 Whole cell lysate at 50 µg

Lane 2:

U2OS Whole cell lysate at 50 µg

Lane 3:

OVCAR-8 Whole cell lysate at 50 µg

Lane 4:

Jurkat Whole cell lysate at 50 µg

Lane 5:

IMR-90 Whole cell lysate at 50 µg

Lane 6:

LNCaP Whole cell lysate at 50 µg

Lane 7:

Hep-G2 Whole cell lysate at 50 µg

Lane 8:

K-562 Whole cell lysate at 50 µg

Lane 9:

GaMG Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR054F

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NT5E aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P21589

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD73 also known as ecto-5'-nucleotidase is an enzyme that functions at the cellular level. The CD73 protein hydrolyzes extracellular nucleotides into nucleosides by removing phosphate groups. It is a 70 kDa glycosylphosphatidylinositol-anchored molecule found on the surface of various cell types including endothelial cells lymphocytes and epithelial cells. Its presence is noted in a wide range of tissues across the human body highlighting its versatile role in cellular communication and metabolism.

Biological function summary

CD73 participates in the regulation of purinergic signaling through adenosine production. It acts in cooperation with other cell surface enzymes such as CD39 forming a functional complex that handles ATP breakdown to adenosine. This enzymatic activity modulates immune responses tissue protection and inflammation control. In addition to immune-related functions CD73 supports the maintenance of vascular integrity and proper cellular adhesion which is important in different physiological and pathological contexts.

Pathways

CD73 plays an important part in the adenosinergic pathway governing the conversion of AMP into adenosine. This activity impacts the signaling and function of the adenosine receptors on immune and non-immune cells. Another important pathway is the angiogenesis process where CD73 contributes by affecting endothelial cell migration and blood vessel development. Relationships with key proteins such as adenosine A2 receptors help facilitate these critical pathway activities.

CD73's involvement with immunosuppression and tumor progression becomes significant. Elevated levels of CD73 are frequently observed in some cancers where it aids in creating a favorable tumor microenvironment through increased adenosine production. This situation assists tumor cells in evading immune detection. Additionally CD73 is linked to autoimmune diseases like multiple sclerosis where it contributes to modulated lymphocyte activity. Connections with proteins such as TGF-beta further illustrate its role in disease-related pathways creating potential targets for therapeutic interventions through the use of anti-CD73 antibodies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed : 21933152, PubMed : 22997138, PubMed : 23142347, PubMed : 24887587, PubMed : 34403084). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed : 34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed : 21933152, PubMed : 22997138, PubMed : 23142347, PubMed : 24887587, PubMed : 34403084).

See full target information NT5E

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD73 antibody [BLR054F] - BSA free

Immunocytochemistry - Anti-CD73 antibody [BLR054F] - BSA free (AB314071)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [BLR054F] - BSA free (AB314071)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [BLR054F] - BSA free (AB314071)

Western blot - Anti-CD73 antibody [BLR054F] - BSA free (AB314071)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise