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Knockout Tested Rabbit Recombinant Monoclonal CD73 antibody. Suitable for Flow Cyt (Intra), WB, IP, ICC/IF and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-CD73 antibody [EPR28213-52] (AB313339), expandable thumbnail
  • Western blot - Anti-CD73 antibody [EPR28213-52] (AB313339), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD73 antibody [EPR28213-52] (AB313339), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD73 antibody [EPR28213-52] (AB313339), expandable thumbnail
  • Immunoprecipitation - Anti-CD73 antibody [EPR28213-52] (AB313339), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)WBIPICC/IFIHC-P
Human
Tested
Tested
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084).

Alternative names

CD73, NT5, NTE, NT5E, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, Thymidylate 5'-phosphatase

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal CD73 antibody. Suitable for Flow Cyt (Intra), WB, IP, ICC/IF and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28213-52
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD73 also known as ecto-5'-nucleotidase is an enzyme that functions at the cellular level. The CD73 protein hydrolyzes extracellular nucleotides into nucleosides by removing phosphate groups. It is a 70 kDa glycosylphosphatidylinositol-anchored molecule found on the surface of various cell types including endothelial cells lymphocytes and epithelial cells. Its presence is noted in a wide range of tissues across the human body highlighting its versatile role in cellular communication and metabolism.

Biological function summary

CD73 participates in the regulation of purinergic signaling through adenosine production. It acts in cooperation with other cell surface enzymes such as CD39 forming a functional complex that handles ATP breakdown to adenosine. This enzymatic activity modulates immune responses tissue protection and inflammation control. In addition to immune-related functions CD73 supports the maintenance of vascular integrity and proper cellular adhesion which is important in different physiological and pathological contexts.

Pathways

CD73 plays an important part in the adenosinergic pathway governing the conversion of AMP into adenosine. This activity impacts the signaling and function of the adenosine receptors on immune and non-immune cells. Another important pathway is the angiogenesis process where CD73 contributes by affecting endothelial cell migration and blood vessel development. Relationships with key proteins such as adenosine A2 receptors help facilitate these critical pathway activities.

Associated diseases and disorders

CD73's involvement with immunosuppression and tumor progression becomes significant. Elevated levels of CD73 are frequently observed in some cancers where it aids in creating a favorable tumor microenvironment through increased adenosine production. This situation assists tumor cells in evading immune detection. Additionally CD73 is linked to autoimmune diseases like multiple sclerosis where it contributes to modulated lymphocyte activity. Connections with proteins such as TGF-beta further illustrate its role in disease-related pathways creating potential targets for therapeutic interventions through the use of anti-CD73 antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-CD73 antibody [EPR28213-52] (ab313339), expandable thumbnail

    Western blot - Anti-CD73 antibody [EPR28213-52] (ab313339)

    CD73 Western blot staining using rabbit Anti-CD73 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Performed under reducing conditions.

    In Western blot, ab313339 was shown to bind specifically to CD73. A band was observed at 68 kDa in wild-type A431 cell lysates whereas loss of signal was observed in the CD73 knockout cell line.

    In Western blot, anti- Vinculin antibody (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) loading control staining at 1/10000 dilution.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-CD73 antibody [EPR28213-52] (ab313339) at 1/1000 dilution

    Lane 1: Wild-type A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 40 µg

    Lane 2: CD73 knockout A431 whole cell lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 63 kDa

    Observed band size: 68 kDa

    Exposure time: 92s

  • Western blot - Anti-CD73 antibody [EPR28213-52] (ab313339), expandable thumbnail

    Western blot - Anti-CD73 antibody [EPR28213-52] (ab313339)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CD73 antibody [EPR28213-52] (ab313339) at 1/1000 dilution

    Lane 1: A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: A375 (Human malignant melanoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: Human spleen tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 63 kDa

    Observed band size: 68 kDa

    Exposure time: 60s

  • Immunocytochemistry/ Immunofluorescence - Anti-CD73 antibody [EPR28213-52] (ab313339), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD73 antibody [EPR28213-52] (ab313339)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U-87 MG (human glioblastoma-astrocytoma epithelial cell) cells labelling CD73 with ab313339 at 1/50 (10.46 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing membranous staining in U-87 MG cells.Negative control: HeLa (PMID: 8566797)Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD73 antibody [EPR28213-52] (ab313339), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD73 antibody [EPR28213-52] (ab313339)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NT5E KO A431 (NT5E knockout human epidermoid carcinoma epithelial cell) cells labelling CD73 with ab313339 at 1/50 (10.46 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing membranous staining in A431 cells and no staining in NT5E knockout A431 cells.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

  • Immunoprecipitation - Anti-CD73 antibody [EPR28213-52] (ab313339), expandable thumbnail

    Immunoprecipitation - Anti-CD73 antibody [EPR28213-52] (ab313339)

    CD73 was immunoprecipitated from 0.35 mg U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate with ab313339 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313339 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate
    Lane 2: ab313339 IP in U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate
    Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab313339 in U-87 MG whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 180 seconds

    All lanes: Immunoprecipitation - Anti-CD73 antibody [EPR28213-52] (ab313339) at 1/30 dilution

    All lanes: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 180s

  • Western blot - Anti-CD73 antibody [EPR28213-52] (ab313339), expandable thumbnail

    Western blot - Anti-CD73 antibody [EPR28213-52] (ab313339)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: HeLa and Raji(PMID: 8566797)
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 180 seconds

    All lanes: Western blot - Anti-CD73 antibody [EPR28213-52] (ab313339) at 1/1000 dilution

    Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 40 µg

    Lane 2: Hela (human cervix adenocarcinoma epithelial cell) whole cell lysate at 40 µg

    Lane 3: Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 68 kDa

    Exposure time: 180s

  • Flow Cytometry (Intracellular) - Anti-CD73 antibody [EPR28213-52] (ab313339), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CD73 antibody [EPR28213-52] (ab313339)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NT5E KO A431 (human NT5E knockout epidermoid carcinoma epithelial cell, Left) / Parental A431(Right) cells labelling CD73 with ab313339 at 1/50 dilution (1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

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