Anti-CD73 antibody [EPR28214-169] (ab314327)

Rabbit Recombinant Monoclonal CD73 antibody. Suitable for WB, IHC-P, mIHC and reacts with Mouse, Rat samples.

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Images

Multiplex immunohistochemistry - Anti-CD73 antibody [EPR28214-169] (AB314327), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	ICC/IF	Flow Cyt	IP	mIHC
Human	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Mouse	Tested	Tested	Not recommended	Not recommended	Not recommended	Tested
Rat	Tested	Tested	Not recommended	Not recommended	Not recommended	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

See full target information Nt5e

Function

Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside (PubMed:8224905). Hydrolyzes IMP (PubMed:8224905). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (By similarity). Although AMP is the preferred substrate can also hydrolyze UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (By similarity).

Alternative names

CD73, Nt5, Nte, Nt5e, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, Thymidylate 5'-phosphatase

Recommended products

Rabbit Recombinant Monoclonal CD73 antibody. Suitable for WB, IHC-P, mIHC and reacts with Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR28214-169
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD73 also known as ecto-5'-nucleotidase is an enzyme that functions at the cellular level. The CD73 protein hydrolyzes extracellular nucleotides into nucleosides by removing phosphate groups. It is a 70 kDa glycosylphosphatidylinositol-anchored molecule found on the surface of various cell types including endothelial cells lymphocytes and epithelial cells. Its presence is noted in a wide range of tissues across the human body highlighting its versatile role in cellular communication and metabolism.

Biological function summary

CD73 participates in the regulation of purinergic signaling through adenosine production. It acts in cooperation with other cell surface enzymes such as CD39 forming a functional complex that handles ATP breakdown to adenosine. This enzymatic activity modulates immune responses tissue protection and inflammation control. In addition to immune-related functions CD73 supports the maintenance of vascular integrity and proper cellular adhesion which is important in different physiological and pathological contexts.

Pathways

CD73 plays an important part in the adenosinergic pathway governing the conversion of AMP into adenosine. This activity impacts the signaling and function of the adenosine receptors on immune and non-immune cells. Another important pathway is the angiogenesis process where CD73 contributes by affecting endothelial cell migration and blood vessel development. Relationships with key proteins such as adenosine A2 receptors help facilitate these critical pathway activities.

Associated diseases and disorders

CD73's involvement with immunosuppression and tumor progression becomes significant. Elevated levels of CD73 are frequently observed in some cancers where it aids in creating a favorable tumor microenvironment through increased adenosine production. This situation assists tumor cells in evading immune detection. Additionally CD73 is linked to autoimmune diseases like multiple sclerosis where it contributes to modulated lymphocyte activity. Connections with proteins such as TGF-beta further illustrate its role in disease-related pathways creating potential targets for therapeutic interventions through the use of anti-CD73 antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Multiplex immunohistochemistry - Anti-CD73 antibody [EPR28214-169] (ab314327)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat lung staining of Surfactant Protein A/PSAP with Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] ab322404 at a 1/5000 (0.092 μg/ml) dilution, F4/80 with Anti-F4/80 antibody [EPR26545-166] ab300421 at 1/5000 (0.070 μg/ml) dilution, and CD73 with ab314327 at 1/500 (0.98 μg/ml), followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-Surfactant Protein A/PSAP (green; Opal™ 520), anti-F4/80 (grey; Opal™ 570) and anti-CD73 (magenta; Opal™ 690) on rat lung.

Panel B: anti-Surfactant Protein A/PSAP staining Clara cells and alveolar type II cells in rat lung.

Panel C: anti-F4/80 staining macrophages in rat lung.

Panel D: anti-CD73 staining epithelium in rat lung.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] ab322404, Anti-F4/80 antibody [EPR26545-166] ab300421 and ab314327 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system..
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-CD73 antibody [EPR28214-169] (ab314327)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse lung staining of Surfactant Protein A/PSAP with Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] ab322404 at a 1/5000 (0.092 μg/ml) dilution, F4/80 with Anti-F4/80 antibody [EPR26545-166] ab300421 at 1/5000 (0.070 μg/ml) dilution, and CD73 with ab314327 at 1/500 (0.98 μg/ml), followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-Surfactant Protein A/PSAP (green; Opal™ 520), anti-F4/80 (grey; Opal™ 570) and anti-CD73 (magenta; Opal™ 690) on mouse lung.

Panel B: anti-Surfactant Protein A/PSAP staining Clara cells and alveolar type II cells in mouse lung.

Panel C: anti-F4/80 staining macrophages in mouse lung.

Panel D: anti-CD73 staining epithelium in mouse lung.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] ab322404, Anti-F4/80 antibody [EPR26545-166] ab300421 and ab314327 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system..
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Western blot - Anti-CD73 antibody [EPR28214-169] (ab314327)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The lane 3 was developed using a high sensitivity ECL substrate.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-CD73 antibody [EPR28214-169] (ab314327) at 1/1000 dilution
Lane 1: Mouse lung tissue lysate at 20 µg
Lane 2: Mouse bladder tissue lysate at 20 µg
Lane 3: Rat lung tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 75 kDa
Exposure time: 70s
Western blot - Anti-CD73 antibody [EPR28214-169] (ab314327)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: RAW264.7 (PMID: 28060378), J774A.1 (PMID: 28060378).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-CD73 antibody [EPR28214-169] (ab314327) at 1/1000 dilution
Lane 1: HC11 (mouse mammary gland epithelial cell) whole cell lysate at 20 µg
Lane 2: 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 4: J774A.1 (mouse reticum cell sarcoma monocyte macrophage) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 75 kDa
Exposure time: 26s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [EPR28214-169] (ab314327)
Immunohistochemical analysis of paraffin-embedded Mouse large B cell lymphoma tissue labeling CD73 with ab314327 at 1/500 (0.98 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mouse large B cell lymphoma.The section was incubated with ab314327 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [EPR28214-169] (ab314327)
Immunohistochemical analysis of paraffin-embedded Mouse breast cancer tissue labeling CD73 with ab314327 at 1/500 (0.98 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mouse breast cancer. The section was incubated with ab314327 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [EPR28214-169] (ab314327)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CD73 with ab314327 at 1/500 (0.98 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on rat spleen. The section was incubated with ab314327 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [EPR28214-169] (ab314327)
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling CD73 with ab314327 at 1/500 (0.98 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on rat liver. The section was incubated with ab314327 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [EPR28214-169] (ab314327)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD73 with ab314327 at 1/500 (0.98 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mouse spleen. The section was incubated with ab314327 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [EPR28214-169] (ab314327)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CD73 with ab314327 at 1/500 (0.98 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mouse liver. The section was incubated with ab314327 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins