Anti-CD73 antibody [EPR28214-169] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [EPR28214-169] - BSA and Azide free (AB314328)

This data was developed using ab314327, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse large B cell lymphoma tissue labeling CD73 with ab314327 at 1/500 (0.98 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mouse large B cell lymphoma.The section was incubated with ab314327 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [EPR28214-169] - BSA and Azide free (AB314328)

This data was developed using ab314327, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling CD73 with ab314327 at 1/500 (0.98 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on rat liver. The section was incubated with ab314327 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [EPR28214-169] - BSA and Azide free (AB314328)

This data was developed using ab314327, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CD73 with ab314327 at 1/500 (0.98 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on rat spleen. The section was incubated with ab314327 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [EPR28214-169] - BSA and Azide free (AB314328)

This data was developed using ab314327, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CD73 with ab314327 at 1/500 (0.98 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mouse liver. The section was incubated with ab314327 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [EPR28214-169] - BSA and Azide free (AB314328)

This data was developed using ab314327, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse breast cancer tissue labeling CD73 with ab314327 at 1/500 (0.98 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mouse breast cancer. The section was incubated with ab314327 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [EPR28214-169] - BSA and Azide free (AB314328)

This data was developed using ab314327, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD73 with ab314327 at 1/500 (0.98 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mouse spleen. The section was incubated with ab314327 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CD73 antibody [EPR28214-169] - BSA and Azide free (AB314328)

This data was developed using ab314327, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse lung staining of Surfactant Protein A/PSAP with ab322404 at a 1/5000 (0.092 μg/ml) dilution, F4/80 with ab300421 at 1/5000 (0.070 μg/ml) dilution, and CD73 with ab314327 at 1/500 (0.98 μg/ml), followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-Surfactant Protein A/PSAP (green; Opal™ 520), anti-F4/80 (grey; Opal™ 570) and anti-CD73 (magenta; Opal™ 690) on mouse lung.
Panel B : anti-Surfactant Protein A/PSAP staining Clara cells and alveolar type II cells in mouse lung.
Panel C : anti-F4/80 staining macrophages in mouse lung.
Panel D : anti-CD73 staining epithelium in mouse lung.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab322404, ab300421 and ab314327 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system..

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CD73 antibody [EPR28214-169] - BSA and Azide free (AB314328)

This data was developed using ab314327, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat liver tissue staining ALDH1L1 with ab307696 at a 1/5000 dilution, ab302928 anti-HIRA/HIR used at 1/100 dilution and ab314327 anti-CD73 used at a 1/250 dilution.

Panel A : merged staining of anti-ALDH1L1 (green; Opal^™520), anti-HIRA/HIR (magenta; Opal^™690) and anti-CD73 (grey; Opal^™570) on rat liver.
Panel B : anti-ALDH1L1 staining cytoplasm of hepatocytes in rat liver.
Panel C : anti-HIRA/HIR staining nucleus of hepatocytes in rat liver.
Panel D : anti-CD73 staining endothelium in rat liver.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307696, ab302928 and ab314327 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CD73 antibody [EPR28214-169] - BSA and Azide free (AB314328)

This data was developed using ab314327, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat lung staining of Surfactant Protein A/PSAP with ab322404 at a 1/5000 (0.092 μg/ml) dilution, F4/80 with ab300421 at 1/5000 (0.070 μg/ml) dilution, and CD73 with ab314327 at 1/500 (0.98 μg/ml), followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-Surfactant Protein A/PSAP (green; Opal™ 520), anti-F4/80 (grey; Opal™ 570) and anti-CD73 (magenta; Opal™ 690) on rat lung.
Panel B : anti-Surfactant Protein A/PSAP staining Clara cells and alveolar type II cells in rat lung.
Panel C : anti-F4/80 staining macrophages in rat lung.
Panel D : anti-CD73 staining epithelium in rat lung.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab322404, ab300421 and ab314327 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system..

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CD73 antibody [EPR28214-169] - BSA and Azide free (AB314328)

This data was developed using ab314327, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse liver tissue staining ALDH1L1 with ab307696 at a 1/5000 dilution, ab302928 anti-HIRA/HIR used at 1/100 dilution and ab314327 anti-CD73 used at a 1/250 dilution.

Panel A : merged staining of anti-ALDH1L1 (green; Opal^™520), anti-HIRA/HIR (magenta; Opal^™690) and anti-CD73 (grey; Opal^™570) on mouse liver.
Panel B : anti-ALDH1L1 staining cytoplasm of hepatocytes in mouse liver.
Panel C : anti-HIRA/HIR staining nucleus of hepatocytes in mouse liver.
Panel D : anti-CD73 staining endothelium in mouse liver.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307696, ab302928 and ab314327 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-CD73 antibody [EPR28214-169] - BSA and Azide free (AB314328)

This data was developed using ab314327, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : RAW264.7 (PMID : 28060378), J774A.1 (PMID : 28060378). In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-CD73 antibody [EPR28214-169] (<a href='/en-us/products/primary-antibodies/cd73-antibody-epr28214-169-ab314327'>ab314327</a>) at 1/1000 dilution

Lane 1:

HC11 (mouse mammary gland epithelial cell) whole cell lysate at 20 µg

Lane 2:

4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 4:

J774A.1 (mouse reticum cell sarcoma monocyte macrophage) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-CD73 antibody [EPR28214-169] - BSA and Azide free (AB314328)

This data was developed using ab314327, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST The lane 3 was developed using a high sensitivity ECL substrate. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-CD73 antibody [EPR28214-169] (<a href='/en-us/products/primary-antibodies/cd73-antibody-epr28214-169-ab314327'>ab314327</a>) at 1/1000 dilution

Lane 1:

Mouse lung tissue lysate at 20 µg

Lane 2:

Mouse bladder tissue lysate at 20 µg

Lane 3:

Rat lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75 kDa

false

Exposure time: 70s