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Mouse Monoclonal CD73 antibody. Carrier free. Suitable for WB, Protein Array, ICC/IF, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NT5E.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [NT5E/2503] - BSA and Azide free (AB257329), expandable thumbnail
  • Western blot - Anti-CD73 antibody [NT5E/2503] - BSA and Azide free (AB257329), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [NT5E/2503] - BSA and Azide free (AB257329), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [NT5E/2503] - BSA and Azide free (AB257329), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [NT5E/2503] - BSA and Azide free (AB257329), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human NT5E. Database link P21589

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBProtein ArrayICC/IFIHC-P
Human
Tested
Expected
Tested
Tested
Recombinant full length protein - Human
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1-2 µg/mL

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1-2 µg/mL

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1-2 µg/mL

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

4 products for Alternative Product

Target data

Function

Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084).

Alternative names

NT5, NTE, NTE, NT5, NT5E, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, Thymidylate 5'-phosphatase

Recommended products

Mouse Monoclonal CD73 antibody. Carrier free. Suitable for WB, Protein Array, ICC/IF, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NT5E.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human NT5E. Database link P21589
Carrier free

Yes

Clone number

NT5E/2503

Purification technique

Affinity purification Protein A/G

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

ab257329 is the carrier-free version of Anti-CD73 antibody [NT5E/2503] ab257309.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD73 also known as ecto-5'-nucleotidase is an enzyme that functions at the cellular level. The CD73 protein hydrolyzes extracellular nucleotides into nucleosides by removing phosphate groups. It is a 70 kDa glycosylphosphatidylinositol-anchored molecule found on the surface of various cell types including endothelial cells lymphocytes and epithelial cells. Its presence is noted in a wide range of tissues across the human body highlighting its versatile role in cellular communication and metabolism.

Biological function summary

CD73 participates in the regulation of purinergic signaling through adenosine production. It acts in cooperation with other cell surface enzymes such as CD39 forming a functional complex that handles ATP breakdown to adenosine. This enzymatic activity modulates immune responses tissue protection and inflammation control. In addition to immune-related functions CD73 supports the maintenance of vascular integrity and proper cellular adhesion which is important in different physiological and pathological contexts.

Pathways

CD73 plays an important part in the adenosinergic pathway governing the conversion of AMP into adenosine. This activity impacts the signaling and function of the adenosine receptors on immune and non-immune cells. Another important pathway is the angiogenesis process where CD73 contributes by affecting endothelial cell migration and blood vessel development. Relationships with key proteins such as adenosine A2 receptors help facilitate these critical pathway activities.

Associated diseases and disorders

CD73's involvement with immunosuppression and tumor progression becomes significant. Elevated levels of CD73 are frequently observed in some cancers where it aids in creating a favorable tumor microenvironment through increased adenosine production. This situation assists tumor cells in evading immune detection. Additionally CD73 is linked to autoimmune diseases like multiple sclerosis where it contributes to modulated lymphocyte activity. Connections with proteins such as TGF-beta further illustrate its role in disease-related pathways creating potential targets for therapeutic interventions through the use of anti-CD73 antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [NT5E/2503] - BSA and Azide free (ab257329), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [NT5E/2503] - BSA and Azide free (ab257329)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD73 using Anti-CD73 antibody [NT5E/2503] ab257309 at 2 μg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD73 antibody [NT5E/2503] ab257309).

  • Western blot - Anti-CD73 antibody [NT5E/2503] - BSA and Azide free (ab257329), expandable thumbnail

    Western blot - Anti-CD73 antibody [NT5E/2503] - BSA and Azide free (ab257329)

    Western blot analysis labeling CD73 with ab257329 at 1 ug/ml.

    All lanes: Western blot - Anti-CD73 antibody [NT5E/2503] - BSA and Azide free (ab257329) at 1 µg/mL

    Lane 1: A431 (Human epidermoid carcinoma cell line) whole cell lysate

    Lane 2: PC3 (Human prostate adenocarcinoma cell line) whole cell lysate

    Predicted band size: 63 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [NT5E/2503] - BSA and Azide free (ab257329), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [NT5E/2503] - BSA and Azide free (ab257329)

    Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for CD73 using Anti-CD73 antibody [NT5E/2503] ab257309 at 2 μg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD73 antibody [NT5E/2503] ab257309).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [NT5E/2503] - BSA and Azide free (ab257329), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [NT5E/2503] - BSA and Azide free (ab257329)

    Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for CD73 using Anti-CD73 antibody [NT5E/2503] ab257309 at 2 μg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD73 antibody [NT5E/2503] ab257309).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [NT5E/2503] - BSA and Azide free (ab257329), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [NT5E/2503] - BSA and Azide free (ab257329)

    Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for CD73 using Anti-CD73 antibody [NT5E/2503] ab257309 at 2 μg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD73 antibody [NT5E/2503] ab257309).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD73 antibody [NT5E/2503] - BSA and Azide free (ab257329), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD73 antibody [NT5E/2503] - BSA and Azide free (ab257329)

    Immunfluorescence staining of U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells stained for CD73 using Anti-CD73 antibody [NT5E/2503] ab257309 at 2 μg/ml followed by goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD73 antibody [NT5E/2503] ab257309).

  • Protein Array - Anti-CD73 antibody [NT5E/2503] - BSA and Azide free (ab257329), expandable thumbnail

    Protein Array - Anti-CD73 antibody [NT5E/2503] - BSA and Azide free (ab257329)

    Analysis of Protein Array containing more than 19,000 full-length human proteins using Anti-CD73 antibody [NT5E/2503] ab257309.

    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD73 antibody [NT5E/2503] ab257309).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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