Anti-CD73 antibody [NT5E/2646]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [NT5E/2646] (AB257312)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD73 using ab257312 at 2 μg/ml in immunohistochemical analysis.

• Flow Cyt

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Flow Cytometry - Anti-CD73 antibody [NT5E/2646] (AB257312)

Flow Cytometric analysis of U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells using ab257312 followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [NT5E/2646] (AB257312)

Formalin-fixed, paraffin-embedded human colon carcinoma tissuse stained for CD73 using ab257312 at 2 μg/ml in immunohistochemical analysis.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CD73 antibody [NT5E/2646] (AB257312)

Immunofluorescence staining of U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells using ab257312 followed by a goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [NT5E/2646] (AB257312)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for CD73 using ab257312 at 2 μg/ml in immunohistochemical analysis.

• WB

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Western blot - Anti-CD73 antibody [NT5E/2646] (AB257312)

All lanes:

Western blot - Anti-CD73 antibody [NT5E/2646] (ab257312) at 2 µg/mL

Lane 1:

A431 (human epidermoid carcinoma cell line) whole cell lysate

Lane 2:

PC-3 (human prostate adenocarcinoma cell line) whole cell lysate

Predicted band size: 63 kDa

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• Protein Array

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Protein Array - Anti-CD73 antibody [NT5E/2646] (AB257312)

Analysis of Protein Array containing more than 19,000 full-length human proteins using ab257312.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.