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AB97479

Anti-CD74 antibody

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(2 Publications)

Rabbit Polyclonal CD74 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CD74 aa 1-50.

View Alternative Names

CD74, DHLAG, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, Ii

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody (AB97479)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody (AB97479)

Immunofluorescence analysis of Raji cells fixed in 4% paraformaldehyde at RT for 15 min labelling CD74 protein using ab97479 at a 1/500 dilution (Green). DAPI was used for nuclear staining (Blue).

Western blot - Anti-CD74 antibody (AB97479)
  • WB

Supplier Data

Western blot - Anti-CD74 antibody (AB97479)

Samples were separated by 15% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-CD74 antibody (ab97479) at 1/1000 dilution

Lane 1:

Jurkat whole cell extract at 30 µg

Lane 2:

Raji whole cell extract at 30 µg

Lane 3:

NCI-H929 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 34 kDa

false

Western blot - Anti-CD74 antibody (AB97479)
  • WB

Supplier Data

Western blot - Anti-CD74 antibody (AB97479)

Samples were separated by 15% SDS-PAGE. The signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-CD74 antibody (ab97479) at 1/1000 dilution

All lanes:

Rat spleen tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 34 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Rat

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human CD74 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P04233

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD74 also known as the invariant chain or li is a protein with a mass of about 33 kDa. It is widely expressed on the surface of immune cells such as B cells dendritic cells and macrophages. CD74 plays an important role in the immune system by acting as a chaperone for major histocompatibility complex class II (MHC II) molecules guiding their migration to endosomes where they encounter antigenic peptides. The CD74 protein also functions as a cell surface receptor for macrophage migration inhibitory factor (MIF) enhancing the immune response process.
Biological function summary

CD74 facilitates multiple immune regulatory processes. It forms a complex with the MHC II molecules aiding in their proper folding and peptide loading in the endoplasmic reticulum. This complex then travels through the Golgi apparatus towards the lysosomal compartments where antigens are presented to initiate adaptive immune responses. In addition CD74 is involved in signal transduction pathways that regulate cell proliferation and survival influenced by interactions with MIF and other molecules.

Pathways

The role of CD74 extends into both the antigen presentation and MIF signaling pathways. Within the antigen presentation pathway CD74's activity is tightly connected with MHC II and subsequently to CD4+ T cells facilitating the activation of these immune cells. The MIF signaling pathway involves CD74 interacting with CD44 a receptor that further propagates the signaling cascades essential for immune modulation and cell survival.

CD74 is linked to inflammatory and autoimmune conditions such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression of CD74 influences the presentation of autoantigens and may contribute to the chronic inflammation seen in these disorders. Its connection to MIF which is a pro-inflammatory cytokine further implicates CD74 in these autoimmune scenarios by promoting persistent immune activation. Researchers are also exploring CD74 as a therapeutic target in certain cancers due to its influence on immune surveillance and tumor progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.. Class-II-associated invariant chain peptide. Binds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome.. Isoform p41. Stabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs). Has antiviral activity by stymieing the endosomal entry of Ebola virus and coronaviruses, including SARS-CoV-2 (PubMed : 32855215). Disrupts cathepsin-mediated Ebola virus glycoprotein processing, which prevents viral fusion and entry. This antiviral activity is specific to p41 isoform (PubMed : 32855215).
See full target information CD74
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Bio-protocol 9:e3254 PubMed33654779

2019

Measurement of CD74 N-terminal Fragment Accumulation in Cells Treated with SPPL2a Inhibitor.

Applications

Unspecified application

Species

Unspecified reactive species

Rubén Martínez-Barricarte,Xiao-Fei Kong,Jean-Laurent Casanova

Nature immunology 19:973-985 PubMed30127434

2018

Disruption of an antimycobacterial circuit between dendritic and helper T cells in human SPPL2a deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Fei Kong,Ruben Martinez-Barricarte,James Kennedy,Federico Mele,Tomi Lazarov,Elissa K Deenick,Cindy S Ma,Gaëlle Breton,Kimberly B Lucero,David Langlais,Aziz Bousfiha,Caner Aytekin,Janet Markle,Céline Trouillet,Fabienne Jabot-Hanin,Cecilia S Lindestam Arlehamn,Geetha Rao,Capucine Picard,Théo Lasseau,Daniela Latorre,Sophie Hambleton,Caroline Deswarte,Yuval Itan,Katia Abarca,Dewton Moraes-Vasconcelos,Fatima Ailal,Aydan Ikinciogullari,Figen Dogu,Ibtihal Benhsaien,Alessandro Sette,Laurent Abel,Stéphanie Boisson-Dupuis,Bernd Schröder,Michel C Nussenzweig,Kang Liu,Frédéric Geissmann,Stuart G Tangye,Philippe Gros,Federica Sallusto,Jacinta Bustamante,Jean-Laurent Casanova
View all publications
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