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Rabbit Recombinant Monoclonal CD74 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P, IHC-Fr and reacts with Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD74 antibody [EPR25399-94] - BSA and Azide free (AB289891), expandable thumbnail
  • Western blot - Anti-CD74 antibody [EPR25399-94] - BSA and Azide free (AB289891), expandable thumbnail
  • Immunoprecipitation - Anti-CD74 antibody [EPR25399-94] - BSA and Azide free (AB289891), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [EPR25399-94] - BSA and Azide free (AB289891), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-CD74 antibody [EPR25399-94] - BSA and Azide free (AB289891), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-PIHC-Fr
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Tested
Tested
Tested
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

-

Not recommended
Not recommended

Species

Rat, Human

Dilution info

-

Notes

-

Target data

Function

Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to compartments where peptide loading of class II takes place. Enhance also the stimulation of T-cell responses through interaction with CD44.Isoform LongStabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs).Class-II-associated invariant chain peptideBinds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal CD74 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P, IHC-Fr and reacts with Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR25399-94

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

ab289891 is a carrier-free version of Anti-CD74 antibody [EPR25399-94] ab289885.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD74 also known as the invariant chain or li is a protein with a mass of about 33 kDa. It is widely expressed on the surface of immune cells such as B cells dendritic cells and macrophages. CD74 plays an important role in the immune system by acting as a chaperone for major histocompatibility complex class II (MHC II) molecules guiding their migration to endosomes where they encounter antigenic peptides. The CD74 protein also functions as a cell surface receptor for macrophage migration inhibitory factor (MIF) enhancing the immune response process.

Biological function summary

CD74 facilitates multiple immune regulatory processes. It forms a complex with the MHC II molecules aiding in their proper folding and peptide loading in the endoplasmic reticulum. This complex then travels through the Golgi apparatus towards the lysosomal compartments where antigens are presented to initiate adaptive immune responses. In addition CD74 is involved in signal transduction pathways that regulate cell proliferation and survival influenced by interactions with MIF and other molecules.

Pathways

The role of CD74 extends into both the antigen presentation and MIF signaling pathways. Within the antigen presentation pathway CD74's activity is tightly connected with MHC II and subsequently to CD4+ T cells facilitating the activation of these immune cells. The MIF signaling pathway involves CD74 interacting with CD44 a receptor that further propagates the signaling cascades essential for immune modulation and cell survival.

Associated diseases and disorders

CD74 is linked to inflammatory and autoimmune conditions such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression of CD74 influences the presentation of autoantigens and may contribute to the chronic inflammation seen in these disorders. Its connection to MIF which is a pro-inflammatory cytokine further implicates CD74 in these autoimmune scenarios by promoting persistent immune activation. Researchers are also exploring CD74 as a therapeutic target in certain cancers due to its influence on immune surveillance and tumor progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD74 antibody [EPR25399-94] - BSA and Azide free (ab289891), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD74 antibody [EPR25399-94] - BSA and Azide free (ab289891)

    This data was developed using Anti-CD74 antibody [EPR25399-94] ab289885, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD74 with Anti-CD74 antibody [EPR25399-94] ab289885 at 1/2000 dilution, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on mouse spleen (PMID: 30191677). Counter stained with Hematoxylin. The section was incubated with Anti-CD74 antibody [EPR25399-94] ab289885 for 30 mins at room temperature.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used.

  • Western blot - Anti-CD74 antibody [EPR25399-94] - BSA and Azide free (ab289891), expandable thumbnail

    Western blot - Anti-CD74 antibody [EPR25399-94] - BSA and Azide free (ab289891)

    This data was developed using Anti-CD74 antibody [EPR25399-94] ab289885, the same antibody clone in a different buffer formulation.

    5% NFDM/TBST was used as blocking and diluting buffer.

    Exposure times:

    Lanes 1-2: 10 seconds;

    Lanes 3-6: 114 seconds

    The observed MW are consistent with what has been described in the literature (PMID: 19413900).

    Negative control: mouse kidney (PMID: 28219888).

    We observe an unknown band at around 60kDa.

    All lanes: Western blot - Anti-CD74 antibody [EPR25399-94] (Anti-CD74 antibody [EPR25399-94] ab289885) at 1/1000 dilution

    Lane 1: Mouse spleen tissue lysate at 20 µg

    Lane 2: Mouse thymus tissue lysate at 20 µg

    Lane 3: Mouse PBMC (Peripheral blood mononuclear cell) tissue lysate at 20 µg

    Lane 4: Mouse liver tissue lysate at 20 µg

    Lane 5: Mouse lung tissue lysate at 20 µg

    Lane 6: Mouse kidney tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 34 kDa

    Observed band size: 27 kDa, 36 kDa

  • Immunoprecipitation - Anti-CD74 antibody [EPR25399-94] - BSA and Azide free (ab289891), expandable thumbnail

    Immunoprecipitation - Anti-CD74 antibody [EPR25399-94] - BSA and Azide free (ab289891)

    This data was developed using Anti-CD74 antibody [EPR25399-94] ab289885, the same antibody clone in a different buffer formulation.

    CD74 was immunoprecipitated from mouse spleen tissue lysate with Anti-CD74 antibody [EPR25399-94] ab289885 at 1/30 dilution (2 μg in 0.35 mg lysates). Western blot was performed from the immunoprecipitate using Anti-CD74 antibody [EPR25399-94] ab289885 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution was used as secondary antibody.

    Lane 1: Mouse spleen tissue lysate 10 μg (Input).

    Lane 2: Anti-CD74 antibody [EPR25399-94] ab289885 IP in Mouse spleen tissue lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD74 antibody [EPR25399-94] ab289885 in mouse spleen tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 6 seconds.

    All lanes: Immunoprecipitation - Anti-CD74 antibody [EPR25399-94] (Anti-CD74 antibody [EPR25399-94] ab289885)

    Predicted band size: 34 kDa

    Observed band size: 27 kDa, 36 kDa

    Exposure time: 6s

  • Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [EPR25399-94] - BSA and Azide free (ab289891), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [EPR25399-94] - BSA and Azide free (ab289891)

    This data was developed using Anti-CD74 antibody [EPR25399-94] ab289885, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 80% methanol fixed, 0.1% Triton X-100 permeabilized A20 (mouse reticulum sarcoma cell line) cells labeling CD74 with Anti-CD74 antibody [EPR25399-94] ab289885 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in A20 cell line. The nuclear counter stain is DAPI (blue).

    Tubulin was labeled using Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (red).

    Secondary antibody only control: PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-CD74 antibody [EPR25399-94] - BSA and Azide free (ab289891), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CD74 antibody [EPR25399-94] - BSA and Azide free (ab289891)

    This data was developed using Anti-CD74 antibody [EPR25399-94] ab289885, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse spleen (fresh) tissue labelling CD74 with Anti-CD74 antibody [EPR25399-94] ab289885 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution (Green). Positive staining on mouse spleen. The nuclear counterstain is DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-CD74 antibody [EPR25399-94] - BSA and Azide free (ab289891), expandable thumbnail

    Western blot - Anti-CD74 antibody [EPR25399-94] - BSA and Azide free (ab289891)

    This data was developed using Anti-CD74 antibody [EPR25399-94] ab289885, the same antibody clone in a different buffer formulation.

    5% NFDM/TBST was used as blocking and diluting buffer.

    Exposure time:

    Lane 1: 6 seconds;

    Lanes 2: 59 seconds

    All lanes: Western blot - Anti-CD74 antibody [EPR25399-94] (Anti-CD74 antibody [EPR25399-94] ab289885) at 1/1000 dilution

    Lane 1: A20 (mouse reticulum sarcoma b lymphocyte) whole cell lysate at 20 µg

    Lane 2: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 34 kDa

    Observed band size: 27 kDa, 36 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD74 antibody [EPR25399-94] - BSA and Azide free (ab289891), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD74 antibody [EPR25399-94] - BSA and Azide free (ab289891)

    This data was developed using Anti-CD74 antibody [EPR25399-94] ab289885, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse lung cancer tissue labeling CD74 with Anti-CD74 antibody [EPR25399-94] ab289885 at 1/2000 dilution, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on mouse lung cancer. Counter stained with Hematoxylin. The section was incubated with Anti-CD74 antibody [EPR25399-94] ab289885 for 30 mins at room temperature.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD74 antibody [EPR25399-94] - BSA and Azide free (ab289891), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD74 antibody [EPR25399-94] - BSA and Azide free (ab289891)

    This data was developed using Anti-CD74 antibody [EPR25399-94] ab289885, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CD74 with Anti-CD74 antibody [EPR25399-94] ab289885 at 1/2000 dilution, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on mouse lung. Counter stained with Hematoxylin. The section was incubated with Anti-CD74 antibody [EPR25399-94] ab289885 for 30 mins at room temperature.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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