AB245692

Anti-CD74 antibody [In-1]

Name: Anti-CD74 antibody [In-1] (ab245692) | Abcam
Brand: Abcam Limited
SKU: AB245692
Price: 555 USD
Availability: InStock
Rating: 5 (1 reviews)

Recombinant
What is this?

(1 Review)

(4 Publications)

Rabbit Recombinant Monoclonal CD74 antibody. Suitable for WB, ICC/IF and reacts with Mouse samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing Cd74 protein.

View Alternative Names

CD74, Ii, Cd74, H-2 class II histocompatibility antigen gamma chain, Ia antigen-associated invariant chain, MHC class II-associated invariant chain, Ii chain

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [In-1] (AB245692)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [In-1] (AB245692)

PFA fixed, 0.15% Triton permeabilized mouse splenocytes labeling CD74 using ab245692 at 10 μg/ml for 1 hour (green) followed by Alexa Fluor^® 488 secondary antibody (1 μg/ml), in ICC/IF. The nuclear counter stain is DAPI (blue).

Supplier Data

Western blot - Anti-CD74 antibody [In-1] (AB245692)

10% SDS PAGE gel.

Detected by chemiluminescence.

All lanes:

Western blot - Anti-CD74 antibody [In-1] (ab245692) at 0.0001 µg/mL

Lane 1:

Mouse spleen lysate (RIPA buffer) at 35 µg

Lane 2:

Mouse lymph node lysate (RIPA buffer) at 35 µg

Predicted band size: 34 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

In-1

Isotype

IgG

Light chain type

kappa

Carrier free

Reacts with

Mouse

Applications

ICC/IF, WB

applications

Immunogen

Cell preparation containing Cd74 protein. The exact immunogen used to generate this antibody is proprietary information.

P04441

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.0001 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Proclin 300 Constituents: 99% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD74 also known as the invariant chain or li is a protein with a mass of about 33 kDa. It is widely expressed on the surface of immune cells such as B cells dendritic cells and macrophages. CD74 plays an important role in the immune system by acting as a chaperone for major histocompatibility complex class II (MHC II) molecules guiding their migration to endosomes where they encounter antigenic peptides. The CD74 protein also functions as a cell surface receptor for macrophage migration inhibitory factor (MIF) enhancing the immune response process.

Biological function summary

CD74 facilitates multiple immune regulatory processes. It forms a complex with the MHC II molecules aiding in their proper folding and peptide loading in the endoplasmic reticulum. This complex then travels through the Golgi apparatus towards the lysosomal compartments where antigens are presented to initiate adaptive immune responses. In addition CD74 is involved in signal transduction pathways that regulate cell proliferation and survival influenced by interactions with MIF and other molecules.

Pathways

The role of CD74 extends into both the antigen presentation and MIF signaling pathways. Within the antigen presentation pathway CD74's activity is tightly connected with MHC II and subsequently to CD4+ T cells facilitating the activation of these immune cells. The MIF signaling pathway involves CD74 interacting with CD44 a receptor that further propagates the signaling cascades essential for immune modulation and cell survival.

CD74 is linked to inflammatory and autoimmune conditions such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression of CD74 influences the presentation of autoantigens and may contribute to the chronic inflammation seen in these disorders. Its connection to MIF which is a pro-inflammatory cytokine further implicates CD74 in these autoimmune scenarios by promoting persistent immune activation. Researchers are also exploring CD74 as a therapeutic target in certain cancers due to its influence on immune surveillance and tumor progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to compartments where peptide loading of class II takes place. Enhance also the stimulation of T-cell responses through interaction with CD44.. Isoform Long. Stabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs).. Class-II-associated invariant chain peptide. Binds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome.

See full target information Cd74

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

BMC oral health 24:407 PubMed38556862

2024

KAT2A-mediated succinylation modification of notch1 promotes the proliferation and differentiation of dental pulp stem cells by activating notch pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Longwei Ye,Zeqin Yu,Lin He,Jie Yuan,Xiaodan Zhang,Lei Li,Xin Huang,Yanyan Ma,Lei Zhang

eLife 12: PubMed37057892

2023

MHC class I and MHC class II reporter mice enable analysis of immune oligodendroglia in mouse models of multiple sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Em P Harrington,Riley B Catenacci,Matthew D Smith,Dongeun Heo,Cecilia E Miller,Keya R Meyers,Jenna Glatzer,Dwight E Bergles,Peter A Calabresi

Nature neuroscience 26:416-429 PubMed36635496

2023

A TREM2-activating antibody with a blood-brain barrier transport vehicle enhances microglial metabolism in Alzheimer's disease models.

Applications

Unspecified application

Species

Unspecified reactive species

Bettina van Lengerich,Lihong Zhan,Dan Xia,Darren Chan,David Joy,Joshua I Park,David Tatarakis,Meredith Calvert,Selina Hummel,Steve Lianoglou,Michelle E Pizzo,Rachel Prorok,Elliot Thomsen,Laura M Bartos,Philipp Beumers,Anja Capell,Sonnet S Davis,Lis de Weerd,Jason C Dugas,Joseph Duque,Timothy Earr,Kapil Gadkar,Tina Giese,Audrey Gill,Johannes Gnörich,Connie Ha,Malavika Kannuswamy,Do Jin Kim,Sebastian T Kunte,Lea H Kunze,Diana Lac,Kendra Lechtenberg,Amy Wing-Sze Leung,Chun-Chi Liang,Isabel Lopez,Paul McQuade,Anuja Modi,Vanessa O Torres,Hoang N Nguyen,Ida Pesämaa,Nicholas Propson,Marvin Reich,Yaneth Robles-Colmenares,Kai Schlepckow,Luna Slemann,Hilda Solanoy,Jung H Suh,Robert G Thorne,Chandler Vieira,Karin Wind-Mark,Ken Xiong,Y Joy Yu Zuchero,Dolo Diaz,Mark S Dennis,Fen Huang,Kimberly Scearce-Levie,Ryan J Watts,Christian Haass,Joseph W Lewcock,Gilbert Di Paolo,Matthias Brendel,Pascal E Sanchez,Kathryn M Monroe

eLife 9: PubMed33054973

2020

A MAC2-positive progenitor-like microglial population is resistant to CSF1R inhibition in adult mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Lihong Zhan,Li Fan,Lay Kodama,Peter Dongmin Sohn,Man Ying Wong,Gergey Alzaem Mousa,Yungui Zhou,Yaqiao Li,Li Gan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD74 antibody [In-1]

Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [In-1] (AB245692)

Western blot - Anti-CD74 antibody [In-1] (AB245692)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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