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Rabbit Recombinant Monoclonal CD74 antibody. Suitable for WB, ICC/IF and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing Cd74 protein.


Images

Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [In-1] (AB245692), expandable thumbnail
  • Western blot - Anti-CD74 antibody [In-1] (AB245692), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.02% Proclin 300
Constituents: 99% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Cell preparation containing Cd74 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P04441

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Mouse
Tested
Tested

Tested
Tested

Species

Mouse

Dilution info

0.0001 µg/mL

Notes

-

Tested
Tested

Species

Mouse

Dilution info

10 µg/mL

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to compartments where peptide loading of class II takes place. Enhance also the stimulation of T-cell responses through interaction with CD44.Isoform LongStabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs).Class-II-associated invariant chain peptideBinds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal CD74 antibody. Suitable for WB, ICC/IF and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing Cd74 protein.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Cell preparation containing Cd74 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P04441
Clone number

In-1

Purification technique

Affinity purification Protein A

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [In-1] (ab245692), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [In-1] (ab245692)

    PFA fixed, 0.15% Triton permeabilized mouse splenocytes labeling CD74 using ab245692 at 10 μg/ml for 1 hour (green) followed by Alexa Fluor® 488 secondary antibody (1 μg/ml), in ICC/IF. The nuclear counter stain is DAPI (blue).

  • Western blot - Anti-CD74 antibody [In-1] (ab245692), expandable thumbnail

    Western blot - Anti-CD74 antibody [In-1] (ab245692)

    10% SDS PAGE gel.

    Detected by chemiluminescence.

    All lanes: Western blot - Anti-CD74 antibody [In-1] (ab245692) at 0.0001 µg/mL

    Lane 1: Mouse spleen lysate (RIPA buffer) at 35 µg

    Lane 2: Mouse lymph node lysate (RIPA buffer) at 35 µg

    Predicted band size: 34 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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