Mouse Monoclonal CD74 antibody. Carrier free. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD74 protein.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD74 antibody [LN-2] - BSA and Azide free (AB213104), expandable thumbnail](https://content.abcam.com/products/images/cd74-antibody-ln-2-bsa-and-azide-free-ab213104--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137791.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD74 antibody [LN-2] - BSA and Azide free (AB213104)")
![Flow Cytometry (Intracellular) - Anti-CD74 antibody [LN-2] - BSA and Azide free (AB213104), expandable thumbnail](https://content.abcam.com/products/images/cd74-antibody-ln-2-bsa-and-azide-free-ab213104--flow-cytometry-intracellular-img51066.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CD74 antibody [LN-2] - BSA and Azide free (AB213104)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing CD74 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P04233
Reactivity data
Select an application| IHC-P | Flow Cyt (Intra) | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes (Primary incubation for 30 minutes at RT) Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes (Primary incubation for 30 minutes at RT) Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 mg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
7 products for Alternative Product
Target data
Function
Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. Class-II-associated invariant chain peptide. Binds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome. Isoform p41. Stabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs). Has antiviral activity by stymieing the endosomal entry of Ebola virus and coronaviruses, including SARS-CoV-2 (PubMed:32855215). Disrupts cathepsin-mediated Ebola virus glycoprotein processing, which prevents viral fusion and entry. This antiviral activity is specific to p41 isoform (PubMed:32855215).
Alternative names
CD74, DHLAG, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, Ii
Recommended products
Mouse Monoclonal CD74 antibody. Carrier free. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD74 protein.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing CD74 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P04233
- Carrier free
- Yes
- Clone number
- LN-2
- Purification technique
- Affinity purification Protein G
- Light chain type
- kappa
- Concentration
- Purification notes
ab213104 was purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD74 also known as the invariant chain or li is a protein with a mass of about 33 kDa. It is widely expressed on the surface of immune cells such as B cells dendritic cells and macrophages. CD74 plays an important role in the immune system by acting as a chaperone for major histocompatibility complex class II (MHC II) molecules guiding their migration to endosomes where they encounter antigenic peptides. The CD74 protein also functions as a cell surface receptor for macrophage migration inhibitory factor (MIF) enhancing the immune response process.
- Biological function summary
CD74 facilitates multiple immune regulatory processes. It forms a complex with the MHC II molecules aiding in their proper folding and peptide loading in the endoplasmic reticulum. This complex then travels through the Golgi apparatus towards the lysosomal compartments where antigens are presented to initiate adaptive immune responses. In addition CD74 is involved in signal transduction pathways that regulate cell proliferation and survival influenced by interactions with MIF and other molecules.
- Pathways
The role of CD74 extends into both the antigen presentation and MIF signaling pathways. Within the antigen presentation pathway CD74's activity is tightly connected with MHC II and subsequently to CD4+ T cells facilitating the activation of these immune cells. The MIF signaling pathway involves CD74 interacting with CD44 a receptor that further propagates the signaling cascades essential for immune modulation and cell survival.
- Associated diseases and disorders
CD74 is linked to inflammatory and autoimmune conditions such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression of CD74 influences the presentation of autoantigens and may contribute to the chronic inflammation seen in these disorders. Its connection to MIF which is a pro-inflammatory cytokine further implicates CD74 in these autoimmune scenarios by promoting persistent immune activation. Researchers are also exploring CD74 as a therapeutic target in certain cancers due to its influence on immune surveillance and tumor progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD74 antibody [LN-2] - BSA and Azide free (ab213104), expandable thumbnail](https://content.abcam.com/products/images/cd74-antibody-ln-2-bsa-and-azide-free-ab213104--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137791.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD74 antibody [LN-2] - BSA and Azide free (ab213104)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD74 antibody [LN-2] - BSA and Azide free (ab213104)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD74 with ab213104 at 2 μg/ml.
![Flow Cytometry (Intracellular) - Anti-CD74 antibody [LN-2] - BSA and Azide free (ab213104), expandable thumbnail](https://content.abcam.com/products/images/cd74-antibody-ln-2-bsa-and-azide-free-ab213104--flow-cytometry-intracellular-img51066.jpg "Flow Cytometry (Intracellular) - Anti-CD74 antibody [LN-2] - BSA and Azide free (ab213104)")
Flow Cytometry (Intracellular) - Anti-CD74 antibody [LN-2] - BSA and Azide free (ab213104)
This data was developed using the same antibody clone in a different buffer formulation (Anti-CD74 antibody [LN2] ab9514)
Flow cytometry overlay histogram showing wild-type Raji (green line) and CD74 knockout Raji cells (Human CD74 knockout Raji cell line ab273378) stained with Anti-CD74 antibody [LN2] ab9514 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (Anti-CD74 antibody [LN2] ab9514) (1x106 in 100μl at 1 μg/ml) for 30 min at 22°C.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) was used at 1/2000 for 30 min at 22°C.
Isotype control antibody was mouse IgG1κ (Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control ab170190) used at the same concentration and conditions as the primary antibody (wild-type Raji cells - black line; CD74 knockout Raji cells Human CD74 knockout Raji cell line ab273378 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
This antibody gave a positive signal in CD74 knockout Raji cells fixed with 4% formaldehyde (10 min) / permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
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