AB265584

Anti-CD74 antibody [LN2]

Name: Anti-CD74 antibody [LN2] (ab265584) | Abcam
Brand: Abcam Limited
SKU: AB265584

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Mouse Monoclonal CD74 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD74 protein.

View Alternative Names

CD74, DHLAG, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, Ii

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD74 antibody [LN2] (AB265584)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD74 antibody [LN2] (AB265584)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for CD74 using ab265584 at 1/100 dilution in immunohistochemical analysis. The secondary antibody was an HRP-conjugated goat anti-mouse IgG. Substrate : DAB

Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [LN2] (AB265584)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [LN2] (AB265584)

Formalin-fixed, paraffin-embedded Daudi cells stained for CD74 using ab265584 at 1/100 dilution in immunocytochemical analysis. The secondary antibody was an HRP-conjugated goat anti-mouse IgG. Substrate : DAB

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD74 antibody [LN2] (AB265584)

Formalin-fixed, paraffin-embedded human lymph node tissue stained for CD74 using ab265584 at 1/100 dilution in immunohistochemical analysis. The secondary antibody was an HRP-conjugated goat anti-mouse IgG. Substrate : DAB

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD74 antibody [LN2] (AB265584)

Formalin-fixed, paraffin-embedded Raji cells stained for CD74 using ab265584 at 1/100 dilution in immunocytochemical analysis. The secondary antibody was an HRP-conjugated goat anti-mouse IgG. Substrate : DAB

Supplier Data

Western blot - Anti-CD74 antibody [LN2] (AB265584)

All lanes:

Western blot - Anti-CD74 antibody [LN2] (ab265584) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 2:

Raji (human Burkitt's lymphoma cell line) whole cell lysate at 50 µg

Lane 3:

GaMG whole cell lysate at 50 µg

Lane 4:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 5:

SR whole cell lysate at 50 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 7:

Daudi (human Burkitt's lymphoma cell line) whole cell lysate at 50 µg

Lane 8:

KG-1 whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-mouse IgG

Predicted band size: 34 kDa

true

Exposure time: 30s

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

LN2

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Cell preparation containing CD74 protein. The exact immunogen used to generate this antibody is proprietary information.

P04233

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections" } } }

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Properties and storage information

Form

Liquid

Purification notes

Purified from cell culture supernatant.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD74 also known as the invariant chain or li is a protein with a mass of about 33 kDa. It is widely expressed on the surface of immune cells such as B cells dendritic cells and macrophages. CD74 plays an important role in the immune system by acting as a chaperone for major histocompatibility complex class II (MHC II) molecules guiding their migration to endosomes where they encounter antigenic peptides. The CD74 protein also functions as a cell surface receptor for macrophage migration inhibitory factor (MIF) enhancing the immune response process.

Biological function summary

CD74 facilitates multiple immune regulatory processes. It forms a complex with the MHC II molecules aiding in their proper folding and peptide loading in the endoplasmic reticulum. This complex then travels through the Golgi apparatus towards the lysosomal compartments where antigens are presented to initiate adaptive immune responses. In addition CD74 is involved in signal transduction pathways that regulate cell proliferation and survival influenced by interactions with MIF and other molecules.

Pathways

The role of CD74 extends into both the antigen presentation and MIF signaling pathways. Within the antigen presentation pathway CD74's activity is tightly connected with MHC II and subsequently to CD4+ T cells facilitating the activation of these immune cells. The MIF signaling pathway involves CD74 interacting with CD44 a receptor that further propagates the signaling cascades essential for immune modulation and cell survival.

CD74 is linked to inflammatory and autoimmune conditions such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression of CD74 influences the presentation of autoantigens and may contribute to the chronic inflammation seen in these disorders. Its connection to MIF which is a pro-inflammatory cytokine further implicates CD74 in these autoimmune scenarios by promoting persistent immune activation. Researchers are also exploring CD74 as a therapeutic target in certain cancers due to its influence on immune surveillance and tumor progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.. Class-II-associated invariant chain peptide. Binds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome.. Isoform p41. Stabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs). Has antiviral activity by stymieing the endosomal entry of Ebola virus and coronaviruses, including SARS-CoV-2 (PubMed : 32855215). Disrupts cathepsin-mediated Ebola virus glycoprotein processing, which prevents viral fusion and entry. This antiviral activity is specific to p41 isoform (PubMed : 32855215).

See full target information CD74

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Product promise

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