Mouse Monoclonal CD75 antibody. Suitable for IP, Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing ST6GAL1 protein.
Images
![Flow Cytometry - Anti-CD75 antibody [B-L5] (AB27316), expandable thumbnail](https://content.abcam.com/products/images/cd75-antibody-b-l5-ab27316--flow-cytometry-img81692.gif "Flow Cytometry - Anti-CD75 antibody [B-L5] (AB27316)")
![Immunoprecipitation - Anti-CD75 antibody [B-L5] (AB27316), expandable thumbnail](https://content.abcam.com/products/images/cd75-antibody-b-l5-ab27316--immunoprecipitation-img9335.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CD75 antibody [B-L5] (AB27316)")
Key facts
- Isotype
- IgM
- Host species
- Mouse
- Storage buffer
pH: 7.3 - 7.5
Preservative: 0.09% Sodium azide
Constituents: 98.91% PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing ST6GAL1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P15907
Reactivity data
Select an application| IP | Flow Cyt | |
|---|---|---|
| Human | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µL for 106 Cells | Notes ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
Associated Products
Select an associated product type
4 products for Alternative Product
1 product for Alternative Version
Target data
Function
Transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates.
Alternative names
SIAT1, ST6GAL1, B-cell antigen CD75, ST6Gal I, Sialyltransferase 1, ST6GalI
Recommended products
Mouse Monoclonal CD75 antibody. Suitable for IP, Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing ST6GAL1 protein.
Key facts
- Isotype
- IgM
- Host species
- Mouse
- Storage buffer
pH: 7.3 - 7.5
Preservative: 0.09% Sodium azide
Constituents: 98.91% PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing ST6GAL1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P15907
- Clone number
- B-L5
- Specificity
Recognises lactosamines.
- Light chain type
- kappa
- Concentration
- Purification notes
This antibody was purified by gel filtration.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD75 also known as ST6GAL1 is an enzyme that belongs to the glycosyltransferase family specifically classified as a sialyltransferase. CD75 catalyzes the addition of sialic acid to glycoproteins and glycolipids impacting their function and stability. It has an approximate molecular mass of 45 kDa. CD75 is expressed in a variety of cell types including B lymphocytes and epithelial cells. Its expression can be observed on the cell surface where it plays important roles in cell-cell interactions.
- Biological function summary
CD75 contributes to the mechanics of cell signaling and immune system modulation. CD75 is involved in the biosynthesis of sialylated glycoconjugates which affect cellular recognition and communication. This enzyme does not form part of a multi-protein complex but interacts with other proteins upon glycosylation events. Through its action CD75 impacts multiple cellular processes including inflammation regulation and pathogen recognition.
- Pathways
CD75 plays roles in glycosylation and immune response mechanisms. It participates in the N-glycan biosynthesis pathway which influences protein folding and stability. Additionally CD75 impacts the sialylation pathway where it modifies the surface proteins for lymphocyte activation. Importantly CD75 interacts with proteins such as Siglecs which are involved in immune response modulation and cellular recognition.
- Associated diseases and disorders
CD75 is implicated in cancer progression and autoimmune diseases. In various cancers such as colon cancer altered CD75 expression affects metastatic potential and tumor cell survival. Additionally aberrant CD75 expression occurs in autoimmune diseases like lupus wherein it modulates immune cell activity. CD75 interacts with proteins like CD22 a sialic acid-binding lectin on B cells influencing disease pathogenesis and progression through immune system pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Flow Cytometry - Anti-CD75 antibody [B-L5] (ab27316)
ab27316, at a dilution of 10µl for 106 cells or 100 µl of whole blood, staining CD75 by Flow Cytometry.
![Immunoprecipitation - Anti-CD75 antibody [B-L5] (ab27316), expandable thumbnail](https://content.abcam.com/products/images/cd75-antibody-b-l5-ab27316--immunoprecipitation-img9335.jpg "Immunoprecipitation - Anti-CD75 antibody [B-L5] (ab27316)")
Immunoprecipitation - Anti-CD75 antibody [B-L5] (ab27316)
CD75 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5μg of Mouse monoclonal to CD75 and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70°C; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab27316.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
Band: 47kDa; CD75
All lanes: Immunoprecipitation - Anti-CD75 antibody [B-L5] (ab27316)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Exposure time: 20min
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