Rabbit Recombinant Monoclonal CD75 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-CD75 antibody [EPR22054-226] - BSA and Azide free (Detector) (AB259500), expandable thumbnail](https://content.abcam.com/products/images/cd75-antibody-epr22054-226-bsa-and-azide-free-detector-ab259500--sandwich-elisa-img169293.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-CD75 antibody [EPR22054-226] - BSA and Azide free (Detector) (AB259500)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates.
Alternative names
SIAT1, ST6GAL1, B-cell antigen CD75, ST6Gal I, Sialyltransferase 1, ST6GalI
Recommended products
Rabbit Recombinant Monoclonal CD75 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22054-226
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab259500 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human ST6Gal1. The recommended pair for sandwich ELISA is:
Capture: Anti-CD75 antibody [EPR22054-287] - BSA and Azide free ab259499, Human ST6Gal1 Capture Antibody (unconjugated)
Detector: ab259500, Human ST6Gal1 Detector Antibody (unconjugated)
The reference range value is 7.81 - 1500 pg/ml.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD75 also known as ST6GAL1 is an enzyme that belongs to the glycosyltransferase family specifically classified as a sialyltransferase. CD75 catalyzes the addition of sialic acid to glycoproteins and glycolipids impacting their function and stability. It has an approximate molecular mass of 45 kDa. CD75 is expressed in a variety of cell types including B lymphocytes and epithelial cells. Its expression can be observed on the cell surface where it plays important roles in cell-cell interactions.
- Biological function summary
CD75 contributes to the mechanics of cell signaling and immune system modulation. CD75 is involved in the biosynthesis of sialylated glycoconjugates which affect cellular recognition and communication. This enzyme does not form part of a multi-protein complex but interacts with other proteins upon glycosylation events. Through its action CD75 impacts multiple cellular processes including inflammation regulation and pathogen recognition.
- Pathways
CD75 plays roles in glycosylation and immune response mechanisms. It participates in the N-glycan biosynthesis pathway which influences protein folding and stability. Additionally CD75 impacts the sialylation pathway where it modifies the surface proteins for lymphocyte activation. Importantly CD75 interacts with proteins such as Siglecs which are involved in immune response modulation and cellular recognition.
- Associated diseases and disorders
CD75 is implicated in cancer progression and autoimmune diseases. In various cancers such as colon cancer altered CD75 expression affects metastatic potential and tumor cell survival. Additionally aberrant CD75 expression occurs in autoimmune diseases like lupus wherein it modulates immune cell activity. CD75 interacts with proteins like CD22 a sialic acid-binding lectin on B cells influencing disease pathogenesis and progression through immune system pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-CD75 antibody [EPR22054-226] - BSA and Azide free (Detector) (ab259500), expandable thumbnail](https://content.abcam.com/products/images/cd75-antibody-epr22054-226-bsa-and-azide-free-detector-ab259500--sandwich-elisa-img169293.jpg "Sandwich ELISA - Anti-CD75 antibody [EPR22054-226] - BSA and Azide free (Detector) (ab259500)")
Sandwich ELISA - Anti-CD75 antibody [EPR22054-226] - BSA and Azide free (Detector) (ab259500)
Representative standard curve from corresponding SimpleStep ELISA® Kit (ab243669).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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