Anti-CD79A antibody [BLR234K] - BSA free (ab314053)

Rabbit Recombinant Monoclonal CD79A antibody. Suitable for Flow Cyt, ICC, IHC-P, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human CD79A aa 150 to C-terminus.

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Images

Western blot - Anti-CD79A antibody [BLR234K] - BSA free (AB314053), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Form: Liquid
Clonality: Monoclonal

Immunogen

Synthetic Peptide within Human CD79A aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P11912

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	Flow Cyt	ICC	IHC-P	IP	WB
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Tested	Tested	Tested	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Target data

See full target information CD79A

Function

Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B-cells.

Alternative names

CD79a, IGA, MB1, CD79A, B-cell antigen receptor complex-associated protein alpha chain, Ig-alpha, MB-1 membrane glycoprotein, Membrane-bound immunoglobulin-associated protein, Surface IgM-associated protein

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Form: Liquid
Clonality: Monoclonal
Immunogen: Synthetic Peptide within Human CD79A aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P11912
Clone number: BLR234K
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: Up to 12 months
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD79a also known as Ig-alpha or MB-1 is a component of the B cell antigen receptor (BCR) complex. It is a transmembrane protein with a molecular mass of approximately 47 kDa. CD79a is part of a heterodimer with CD79b and is mainly expressed on the surface of B lymphocytes. This protein plays a critical mechanical role in signaling transduction through the BCR by associating with membrane-bound immunoglobulin molecules helping to initiate the immune response upon antigen binding.

Biological function summary

This protein acts as an essential mediator in the immune system. CD79a together with CD79b forms part of the BCR complex critical for B cell development and maturation. The BCR complex activates signaling cascades that influence B cell fate decisions such as proliferation differentiation and apoptosis. By transmitting signals from the BCR CD79a helps regulate these cellular functions necessary for efficient immune defense.

Pathways

CD79a plays a pivotal role in the B cell receptor signaling pathway. This pathway involves signaling molecules such as spleen tyrosine kinase (SYK) and phosphoinositide 3-kinase (PI3K) which are activated upon BCR engagement. CD79a acts in concert with other proteins including CD79b and the IgM receptor facilitating downstream signaling cascades that ensure proper B cell responses. Its role is important for linking extracellular antigen recognition to intracellular signaling events.

Associated diseases and disorders

CD79a is significant in conditions like B cell lymphomas and certain immunodeficiencies. Abnormal expression or mutations in CD79a are associated with some types of non-Hodgkin lymphoma. The protein's interaction with CD79b is particularly relevant as alterations in these proteins' function or expression can impact B cell development and lead to pathogenesis in lymphoproliferative disorders. Consequently CD79a serves as an important biomarker and potential therapeutic target in these diseases.

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9 product images

Western blot - Anti-CD79A antibody [BLR234K] - BSA free (ab314053)
Samples: Whole cell lysate (50 µg) from WEHI-231 (25 µg), NIH 3T3, CH27, TCMK-1, and A20 cells prepared using NETN lysis buffer. Antibody:Rabbit anti-CD79A recombinant monoclonal antibody [BLR234K] (A700-234 lot 1) used at 1:1000. Secondary:HRP-conjugated goat anti-rabbit IgG (A120-101P). Detection:Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit antiActin recombinant monoclonal antibody [BLR057F] (A700-057).
All lanes: Western blot - Anti-CD79A antibody [BLR234K] - BSA free (ab314053) at 1/1000 dilution
Lane 1: WEHI-231 Whole cell lysate at 25 µg
Lane 2: NIH 3T3 Whole cell lysate at 25 µg
Lane 3: CH 27 Whole cell lysate at 25 µg
Lane 4: TCMK-1 Whole cell lysate at 25 µg
Lane 5: A 20 Whole cell lysate at 25 µg
Secondary
All lanes: HRP-conjugated goat anti-rabbit IgG
Western blot - Anti-CD79A antibody [BLR234K] - BSA free (ab314053)
Samples: Whole cell lysate (50 µg) from Daudi, HeLa, Raji, HEK293T, and Ramos cells prepared using NETN lysis buffer. Antibody:Rabbit anti-CD79A recombinant monoclonal antibody [BLR234K] (A700-234 lot 1) used at 1:1000. Secondary:HRP-conjugated goat anti-rabbit IgG (A120-101P). Detection:Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit antiActin recombinant monoclonal antibody [BLR057F] (A700-057).
All lanes: Western blot - Anti-CD79A antibody [BLR234K] - BSA free (ab314053) at 1/1000 dilution
Lane 1: Daudi Whole cell lysate at 50 µg
Lane 2: Hela Whole cell lysate at 50 µg
Lane 3: Raji Whole cell lysate at 50 µg
Lane 4: HEK- 293T Whole cell lysate at 50 µg
Lane 5: Ramos Whole cell lysate at 50 µg
Secondary
All lanes: HRP-conjugated goat anti-rabbit IgG
Immunoprecipitation - Anti-CD79A antibody [BLR234K] - BSA free (ab314053)
Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Ramos cells prepared using NETN lysis buffer. Antibodies:Rabbit antiCD79A recombinant monoclonal antibody [BLR234K] (A700-234 lot 1) used for IP at 20 µl/mg lysate. CD79A was also immunoprecipitated by a second antibody against a different epitope of CD79A (A700-232). For blotting immunoprecipitated CD79A, A700-234 was used at 1:1000. Detection:Chemiluminescence with an exposure time of 1 second.
Flow Cytometry - Anti-CD79A antibody [BLR234K] - BSA free (ab314053)
Detection of mouse CD79A (shaded) in A20 cells by flow cytometry.Antibody:Rabbit anti-CD79A recombinant monoclonal antibody [BLR234K]
Flow Cytometry - Anti-CD79A antibody [BLR234K] - BSA free (ab314053)
Detection of human CD79A (shaded) in Daudi cells by flow cytometry.Antibody:Rabbit anti-CD79A recombinant monoclonal antibody [BLR234K] or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Immunocytochemistry - Anti-CD79A antibody [BLR234K] - BSA free (ab314053)
Detection of human CD79A by immunocytochemistry. Sample: FFPE section of Daudi cells. Antibody: Rabbit anti-CD79A recombinant monoclonal antibody [BLR234K] Secondary: HRP-conjugated goat anti-rabbit IgG .
Immunocytochemistry - Anti-CD79A antibody [BLR234K] - BSA free (ab314053)
Detection of mouse CD79A by immunocytochemistry Sample:FFPE section of WEHI-231 cells. Antibody:Rabbit anti-CD79A recombinant monoclonal antibody [BLR234K] (A700-234-1). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P).
Immunohistochemistry - Anti-CD79A antibody [BLR234K] - BSA free (ab314053)
Detection of human CD79A by immunohistochemistry Sample: FFPE section of human tonsil. Antibody: Rabbit anti-CD79A recombinant monoclonal antibody [BLR234K] (A700-234-1). Secondary: HRP-conjugated goat antirabbit IgG (A120-501P).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79A antibody [BLR234K] - BSA free (ab314053)
Sample:FFPE section of mouse spleen. Antibody:Rabbit anti-CD79A recombinant monoclonal antibody [BLR234K] (A700-234-1). Secondary:HRP-conjugated goat antirabbit IgG (A120-501P).