Anti-CD79a antibody [EP3618]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (AB79414)

ab79414 showing positive staining in Normal tonsil tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (AB79414)

ab79414 showing positive staining in Normal spleen tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD79a antibody [EP3618] (AB79414)

Immunocytochemistry/Immunofluorescence analysis of RAMOS (human Burkitt's lymphoma) cells labelling CD79a with purified ab79414 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

Secondary Only Control : PBS was used instead of the primary antibody as the negative control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (AB79414)

ab79414 at 1/100 dilution staining CD79a in paraffin-embedded human tonsil tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-CD79a antibody [EP3618] (AB79414)

False colour image of Western blot : Anti-CD79a antibody [EP3618] staining at 1/5000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab79414 was shown to bind specifically to CD79a. A band was observed at 40-50 kDa in wild-type Raji cell lysates with no signal observed at this size in CD79A knockout cell line ab274911 (knockout cell lysate ab281361). To generate this image, wild-type and CD79A knockout Raji cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-CD79a antibody [EP3618] (ab79414) at 1/5000 dilution

Lane 1:

Wild-type Raji cell lysate at 20 µg

Lane 2:

CD79A knockout Raji cell lysate at 20 µg

Lane 2:

Western blot - Human CD791 knockout Raji cell line (<a href='/en-us/products/cell-lines/human-cd791-knockout-raji-cell-line-ab274911'>ab274911</a>)

Lane 3:

Daudi cell lysate at 20 µg

Lane 4:

HAP1 cell lysate at 20 µg

Predicted band size: 25 kDa

Observed band size: 40-50 kDa

false

• WB

Unknown

Western blot - Anti-CD79a antibody [EP3618] (AB79414)

All lanes:

Western blot - Anti-CD79a antibody [EP3618] (ab79414) at 1/10000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

Daudi cell lysate at 10 µg

Lane 3:

Human tonsil tissue lysate at 10 µg

Lane 4:

Human spleen tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 25 kDa

Observed band size: 44-48 kDa

false

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (AB79414)

ab79414 showing negative staining in Normal colon tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (AB79414)

ab79414 showing negative staining in Normal heart tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (AB79414)

ab79414 showing negative staining in Normal brain tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-CD79a antibody [EP3618] (AB79414)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.