Anti-CD79a antibody [EPR26537-114]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CD79a antibody [EPR26537-114] (AB300150)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized Mouse splenocytes cells lebelling CD79a with ab300150 at 1/100 (6.16 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing membranous and cytoplasmic staining in subsets of mouse splenocytes. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5 µg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EPR26537-114] (AB300150)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling CD79a with ab300150 at 1/5000 (0.123 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Negative control : no staining on mouse cerebrum. The section was incubated with ab300150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : PBS was used instead of primary antibody (ab300150) followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EPR26537-114] (AB300150)

Immunohistochemical analysis of paraffin-embedded Mouse large B-cell lymphoma tissue labelling CD79a with ab300150 at 1/5000 (0.123 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Membraneous staining on mouse large B-cell lymphoma. The section was incubated with ab300150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : PBS was used instead of ab300150 followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EPR26537-114] (AB300150)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labelling CD79a with ab300150 at 1/5000 (0.123 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Membraneous staining on rat spleen.The section was incubated with ab300150 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : PBS was used instead of primary antibody (ab300150) followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EPR26537-114] (AB300150)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling CD79a with ab300150 at 1/5000 (0.123 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Negative control : no staining on rat cerebrum.The section was incubated with ab300150 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : PBS was used instead of primary antibody (ab300150) followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EPR26537-114] (AB300150)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling CD79a with ab300150 at 1/5000 (0.123 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Membraneous staining on mouse spleen.The section was incubated with ab300150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : PBS was used instead of ab300150 followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-CD79a antibody [EPR26537-114] (AB300150)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen (fresh) tissue labeling CD79a with ab300150 at 1/500 (1.232 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/mL) dilution (Green). Positive staining on mouse spleen is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbedat 1/1000 (2 µg/mL) dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-CD79a antibody [EPR26537-114] (AB300150)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat spleen (fresh) tissue labeling CD79a with ab300150 at 1/500 (1.232 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/mL) dilution (Green). Positive staining on rat spleen is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbedat 1/1000 (2 µg/mL) dilution.

• IP

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Immunoprecipitation - Anti-CD79a antibody [EPR26537-114] (AB300150)

CD79a was immunoprecipitated from 0.35 mg Mouse lymph node tissue lysate 10 µg with ab300150 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300150 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-CD79a antibody [EPR26537-114] (ab300150)

Lane 1:

Mouse lymph node tissue lysate (Input) at 10 µg

Lane 2:

ab300150 IP in Mouse lymph node tissue lysate (+)

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab300150 in Mouse lymph node tissue lysate (-)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa,30-50 kDa

false

Exposure time: 15s

• IP

Supplier Data

Immunoprecipitation - Anti-CD79a antibody [EPR26537-114] (AB300150)

CD79a was immunoprecipitated from 0.35 mg rat spleen tissue lysate 10 µg with ab300150 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300150 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-CD79a antibody [EPR26537-114] (ab300150)

Lane 1:

Rat spleen tissue lysate (Input) at 10 µg

Lane 2:

ab300150 IP in rat spleen tissue lysate (+)

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab300150 in Rat spleen tissue lysate (-)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa,30-50 kDa

false

Exposure time: 15s

• WB

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Western blot - Anti-CD79a antibody [EPR26537-114] (AB300150)

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Low expression : brain, skin(Human Protein Atlas).

The molecular weight observed is consistent with what has been described in the literature (PMID : 15591116).

All lanes:

Western blot - Anti-CD79a antibody [EPR26537-114] (ab300150) at 1/1000 dilution

Lane 1:

Rat lymph node tissue lysate at 20 µg

Lane 2:

Rat spleen tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Lane 4:

Rat skin tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa,30-50 kDa

false

Exposure time: 81s

• WB

Supplier Data

Western blot - Anti-CD79a antibody [EPR26537-114] (AB300150)

Blocking and dilution buffer and concentration : 5% NFDM/TBST

Low expression : C2C12 (database : Harmonizome).

The molecular weight observed is consistent with what has been described in the literature (PMID : 15591116).

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-CD79a antibody [EPR26537-114] (ab300150) at 1/1000 dilution

Lane 1:

A20 (Mouse reticulum sarcoma B lymphocyte) whole cell lysate at 20 µg

Lane 2:

C2C12 (Mouse myoblasts myoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa,30-50 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-CD79a antibody [EPR26537-114] (AB300150)

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

CD79a is a glycosylated protein and can be deglycosylated by Protein Deglycosylation MIX II.

The molecular weight observed is consistent with what has been described in the literature (PMID : 15591116).

All lanes:

Western blot - Anti-CD79a antibody [EPR26537-114] (ab300150) at 1/1000 dilution

Lane 1:

Untreated Rat lymph node tissue lysate at 15 µg

Lane 2:

Rat lymph node tissue lysate treated with Protein Deglycosylation MIX II at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa,30-50 kDa

false

Exposure time: 92s

• WB

Supplier Data

Western blot - Anti-CD79a antibody [EPR26537-114] (AB300150)

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Low expression : brain, skin(Human Protein Atlas).

The molecular weight observed is consistent with what has been described in the literature (PMID : 15591116).

All lanes:

Western blot - Anti-CD79a antibody [EPR26537-114] (ab300150) at 1/1000 dilution

Lane 1:

Mouse lymph node tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse skin tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 25 kDa,30-50 kDa

Observed band size: 25 kDa

false

Exposure time: 81s