Anti-CD79a antibody [EPR26537-114] (ab300150)

Rabbit Recombinant Monoclonal CD79A antibody. Suitable for WB, IHC-P, IHC-Fr, ICC/IF, IP and reacts with Mouse, Rat samples.

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Images

Immunoprecipitation - Anti-CD79a antibody [EPR26537-114] (AB300150), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	IHC-Fr	ICC/IF	Flow Cyt	IP
Human	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Mouse	Tested	Tested	Tested	Tested	Not recommended	Tested
Rat	Tested	Tested	Tested	Expected	Not recommended	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes -
Species Rat	Dilution info 1/500	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes -
Species Rat	Dilution info 1/30	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Target data

See full target information Cd79a

Function

Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B-cells.

Alternative names

CD79a, Iga, Mb-1, Cd79a, B-cell antigen receptor complex-associated protein alpha chain, Ig-alpha, MB-1 membrane glycoprotein, Membrane-bound immunoglobulin-associated protein, Surface IgM-associated protein

Recommended products

Rabbit Recombinant Monoclonal CD79A antibody. Suitable for WB, IHC-P, IHC-Fr, ICC/IF, IP and reacts with Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR26537-114
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD79a also known as Ig-alpha or MB-1 is a component of the B cell antigen receptor (BCR) complex. It is a transmembrane protein with a molecular mass of approximately 47 kDa. CD79a is part of a heterodimer with CD79b and is mainly expressed on the surface of B lymphocytes. This protein plays a critical mechanical role in signaling transduction through the BCR by associating with membrane-bound immunoglobulin molecules helping to initiate the immune response upon antigen binding.

Biological function summary

This protein acts as an essential mediator in the immune system. CD79a together with CD79b forms part of the BCR complex critical for B cell development and maturation. The BCR complex activates signaling cascades that influence B cell fate decisions such as proliferation differentiation and apoptosis. By transmitting signals from the BCR CD79a helps regulate these cellular functions necessary for efficient immune defense.

Pathways

CD79a plays a pivotal role in the B cell receptor signaling pathway. This pathway involves signaling molecules such as spleen tyrosine kinase (SYK) and phosphoinositide 3-kinase (PI3K) which are activated upon BCR engagement. CD79a acts in concert with other proteins including CD79b and the IgM receptor facilitating downstream signaling cascades that ensure proper B cell responses. Its role is important for linking extracellular antigen recognition to intracellular signaling events.

Associated diseases and disorders

CD79a is significant in conditions like B cell lymphomas and certain immunodeficiencies. Abnormal expression or mutations in CD79a are associated with some types of non-Hodgkin lymphoma. The protein's interaction with CD79b is particularly relevant as alterations in these proteins' function or expression can impact B cell development and lead to pathogenesis in lymphoproliferative disorders. Consequently CD79a serves as an important biomarker and potential therapeutic target in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

Immunoprecipitation - Anti-CD79a antibody [EPR26537-114] (ab300150)
CD79a was immunoprecipitated from 0.35 mg rat spleen tissue lysate 10 µg with ab300150 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300150 at 1/1000 dilution.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Lanes 1 - 2: Immunoprecipitation - Anti-CD79a antibody [EPR26537-114] (ab300150)
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
Lane 1: Rat spleen tissue lysate (Input) at 10 µg
Lane 2: ab300150 IP in rat spleen tissue lysate (+)
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab300150 in Rat spleen tissue lysate (-)
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa, 30-50 kDa
Exposure time: 15s
Immunoprecipitation - Anti-CD79a antibody [EPR26537-114] (ab300150)
CD79a was immunoprecipitated from 0.35 mg Mouse lymph node tissue lysate 10 µg with ab300150 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300150 at 1/1000 dilution.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Lanes 1 - 2: Immunoprecipitation - Anti-CD79a antibody [EPR26537-114] (ab300150)
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
Lane 1: Mouse lymph node tissue lysate (Input) at 10 µg
Lane 2: ab300150 IP in Mouse lymph node tissue lysate (+)
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab300150 in Mouse lymph node tissue lysate (-)
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa, 30-50 kDa
Exposure time: 15s
Western blot - Anti-CD79a antibody [EPR26537-114] (ab300150)
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Low expression: brain, skin(Human Protein Atlas).
The molecular weight observed is consistent with what has been described in the literature (PMID:15591116).
All lanes: Western blot - Anti-CD79a antibody [EPR26537-114] (ab300150) at 1/1000 dilution
Lane 1: Rat lymph node tissue lysate at 20 µg
Lane 2: Rat spleen tissue lysate at 20 µg
Lane 3: Rat brain tissue lysate at 20 µg
Lane 4: Rat skin tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa, 30-50 kDa
Exposure time: 81s
Immunocytochemistry/ Immunofluorescence - Anti-CD79a antibody [EPR26537-114] (ab300150)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized Mouse splenocytes cells lebelling CD79a with ab300150 at 1/100 (6.16 µg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing membranous and cytoplasmic staining in subsets of mouse splenocytes. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5 µg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.
Immunohistochemistry (Frozen sections) - Anti-CD79a antibody [EPR26537-114] (ab300150)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen (fresh) tissue labeling CD79a with ab300150 at 1/500 (1.232 µg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/mL) dilution (Green). Positive staining on mouse spleen is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbedat 1/1000 (2 µg/mL) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EPR26537-114] (ab300150)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling CD79a with ab300150 at 1/5000 (0.123 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Membraneous staining on mouse spleen.The section was incubated with ab300150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of ab300150 followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EPR26537-114] (ab300150)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labelling CD79a with ab300150 at 1/5000 (0.123 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Membraneous staining on rat spleen.The section was incubated with ab300150 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody (ab300150) followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-CD79a antibody [EPR26537-114] (ab300150)
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Low expression: brain, skin(Human Protein Atlas).
The molecular weight observed is consistent with what has been described in the literature (PMID:15591116).
All lanes: Western blot - Anti-CD79a antibody [EPR26537-114] (ab300150) at 1/1000 dilution
Lane 1: Mouse lymph node tissue lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Mouse skin tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 25 kDa, 30-50 kDa
Observed band size: 25 kDa
Exposure time: 81s
Western blot - Anti-CD79a antibody [EPR26537-114] (ab300150)
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
CD79a is a glycosylated protein and can be deglycosylated by Protein Deglycosylation MIX II.
The molecular weight observed is consistent with what has been described in the literature (PMID:15591116).
All lanes: Western blot - Anti-CD79a antibody [EPR26537-114] (ab300150) at 1/1000 dilution
Lane 1: Untreated Rat lymph node tissue lysate at 15 µg
Lane 2: Rat lymph node tissue lysate treated with Protein Deglycosylation MIX II at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa, 30-50 kDa
Exposure time: 92s
Immunohistochemistry (Frozen sections) - Anti-CD79a antibody [EPR26537-114] (ab300150)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat spleen (fresh) tissue labeling CD79a with ab300150 at 1/500 (1.232 µg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/mL) dilution (Green). Positive staining on rat spleen is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbedat 1/1000 (2 µg/mL) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EPR26537-114] (ab300150)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling CD79a with ab300150 at 1/5000 (0.123 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Negative control: no staining on rat cerebrum.The section was incubated with ab300150 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody (ab300150) followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EPR26537-114] (ab300150)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling CD79a with ab300150 at 1/5000 (0.123 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Negative control: no staining on mouse cerebrum. The section was incubated with ab300150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody (ab300150) followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EPR26537-114] (ab300150)
Immunohistochemical analysis of paraffin-embedded Mouse large B-cell lymphoma tissue labelling CD79a with ab300150 at 1/5000 (0.123 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Membraneous staining on mouse large B-cell lymphoma. The section was incubated with ab300150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of ab300150 followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-CD79a antibody [EPR26537-114] (ab300150)
Blocking and dilution buffer and concentration: 5% NFDM/TBST
Low expression: C2C12 (database: Harmonizome).
The molecular weight observed is consistent with what has been described in the literature (PMID: 15591116).
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-CD79a antibody [EPR26537-114] (ab300150) at 1/1000 dilution
Lane 1: A20 (Mouse reticulum sarcoma B lymphocyte) whole cell lysate at 20 µg
Lane 2: C2C12 (Mouse myoblasts myoblast) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 25 kDa
Observed band size: 25 kDa, 30-50 kDa
Exposure time: 180s