Anti-CD79B antibody [BLR229K] - BSA free (ab314044)

Rabbit Recombinant Monoclonal CD79b antibody. Suitable for Flow Cyt (Intra), ICC, IHC-P, IP, WB and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human CD79B aa 200 to C-terminus.

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Images

Western blot - Anti-CD79B antibody [BLR229K] - BSA free (AB314044), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Form: Liquid
Clonality: Monoclonal

Immunogen

Synthetic Peptide within Human CD79B aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P40259

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	Flow Cyt (Intra)	ICC	IHC-P	IP	WB
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Tested	Tested	Tested	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Target data

See full target information CD79B

Function

Required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.

Alternative names

CD79b, B29, IGB, CD79B, B-cell antigen receptor complex-associated protein beta chain, B-cell-specific glycoprotein B29, Ig-beta, Immunoglobulin-associated B29 protein

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Form: Liquid
Clonality: Monoclonal
Immunogen: Synthetic Peptide within Human CD79B aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P40259
Clone number: BLR229K
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: Up to 12 months
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD79b also known as Ig-beta is a protein that plays a mechanical role as part of the B-cell antigen receptor complex. This protein with a mass of approximately 40 kDa is mainly expressed on the surface of B lymphocytes. It is an important component of the heterodimeric complex composed of CD79a (Ig-alpha) and CD79b which associates with membrane-bound immunoglobulins to form the B-cell receptor (BCR). CD79b is essential for transducing activation signals following antigen binding to the BCR.

Biological function summary

The primary role of CD79b within the immune system is to support signal transduction. Upon binding of an antigen to BCR on B cells CD79b as part of the BCR complex initiates signaling cascades which lead to B-cell activation proliferation and differentiation. These processes are critical for humoral immunity ensuring adequate antibody production. CD79b is often found in association with CD19 another important B-cell surface protein and together they aid in amplifying the signals required for B-cell response.

Pathways

CD79b is deeply involved in the B-cell receptor signaling pathway and the broader adaptive immune response. Once the BCR is engaged by an antigen CD79b helps activate intracellular signaling pathways that involve kinases such as SYK and BLNK which help propagate and amplify the immune response. These pathways also interact with other receptor components like CD22 and CD19 creating a network of interactions ensuring effective pathogen recognition and response.

Associated diseases and disorders

CD79b carries significant relevance in the context of B-cell malignancies such as chronic lymphocytic leukemia (CLL) and some types of lymphoma. Dysregulation or mutations in CD79b can affect BCR signaling leading to unchecked B-cell proliferation characteristic of these diseases. Additionally CD79b's interaction with proteins such as SYK in the signaling pathways makes it a potential therapeutic target in treating B-cell related cancers. Researchers are actively investigating anti-CD79b therapies to disrupt abnormal signaling in B-cell malignancies.

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9 product images

Western blot - Anti-CD79B antibody [BLR229K] - BSA free (ab314044)
Samples: Whole cell lysate (50 µg) from Daudi, HeLa, Raji, and Jurkat cells prepared using NETN lysis buffer. Antibody:Rabbit anti-CD79B recombinant monoclonal antibody [BLR229K] (A700-229 lot 1) used at 1:1000. Secondary:HRPconjugated goat anti-rabbit IgG (A120-101P). Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F] (A700-057).
All lanes: Western blot - Anti-CD79B antibody [BLR229K] - BSA free (ab314044) at 1/1000 dilution
Lane 1: Daudi (Human Burkitt's lymphoma cell line) whole cell lysate at 50 µg
Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 3: Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 50 µg
Lane 4: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Secondary
All lanes: HRP-conjugated goat anti-rabbit IgG
Western blot - Anti-CD79B antibody [BLR229K] - BSA free (ab314044)
This data was developed using the standard version of ab314044, the same antibody clone in a different buffer formulation.

Lysates prepared using NETN lysis buffer.

Detection: Chemiluminescence with an exposure time of 10 seconds.

Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].
All lanes: Western blot - Anti-CD79B antibody [BLR229K] - BSA free (ab314044) at 1/1000 dilution
Lane 1: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 50 µg
Lane 2: CH27 (Mouse B cell lymphoma cell line) whole cell lysate at 50 µg
Lane 3: CT26 (Mouse colorectal carcinoma cell line) whole cell lysate at 50 µg
Lane 4: WEHI-231 (Mouse B lymphoma cell line) whole cell lysate at 50 µg
Lane 5: TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg
Secondary
All lanes: HRP-conjugated goat anti-rabbit IgG
Immunoprecipitation - Anti-CD79B antibody [BLR229K] - BSA free (ab314044)
Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Raji cells prepared using NETN lysis buffer. Antibodies:Rabbit anti-CD79B recombinant monoclonal antibody [BLR229K] (A700-229 lot 1) used for IP at 20 µl/mg lysate. CD79B was also immunoprecipitated by a second antibody against a different epitope of CD79B (Antibody 2). For blotting immunoprecipitated CD79B, A700-229 was used at 1:1000. Detection:Chemiluminescence with an exposure time of 3 seconds.
All lanes: Immunoprecipitation - Anti-CD79B antibody [BLR229K] - BSA free (ab314044)
Immunocytochemistry - Anti-CD79B antibody [BLR229K] - BSA free (ab314044)
This data was developed using the standard version of ab314044, the same antibody clone in a different buffer formulation.

Sample: FFPE section of Daudi (Human Burkitt's lymphoma cell line) cells.

Antibody: Standard version of ab314044 at a 1/100 dilution.

Secondary: HRP-conjugated goat anti-rabbit IgG.

Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Immunocytochemistry - Anti-CD79B antibody [BLR229K] - BSA free (ab314044)
This data was developed using the standard version of ab314044, the same antibody clone in a different buffer formulation.

Sample: FFPE section of CH27 (Mouse B cell lymphoma cell line) cells.

Antibody: Standard version of ab314044 at a 1/100 dilution.

Secondary: HRP-conjugated goat anti-rabbit IgG.

Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79B antibody [BLR229K] - BSA free (ab314044)
This data was developed using the standard version of ab314044, the same antibody clone in a different buffer formulation.
Sample: FFPE section of human spleen.
Antibody: Standard version of ab314044 at a 1/100 dilution.
Secondary: HRP-conjugated goat anti-rabbit IgG.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79B antibody [BLR229K] - BSA free (ab314044)
This data was developed using the standard version of ab314044, the same antibody clone in a different buffer formulation.
Sample: FFPE section of mouse spleen.
Antibody: Standard version of ab314044 at a 1/100 dilution.
Secondary: HRP-conjugated goat anti-rabbit IgG.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Flow Cytometry (Intracellular) - Anti-CD79B antibody [BLR229K] - BSA free (ab314044)
This data was developed using the standard version of ab314044, the same antibody clone in a different buffer formulation.
Detection of human CD78B( shaded) in Daudi (Human Burkitt's lymphoma cell line) cells by Flow Cytometry (Intracellular).
Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells.
Antibody: Standard version of ab314044 (shaded red) or isotype control (unshaded).
Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Flow Cytometry (Intracellular) - Anti-CD79B antibody [BLR229K] - BSA free (ab314044)
This data was developed using the standard version of ab314044, the same antibody clone in a different buffer formulation.
Detection of mouse CD78B in A20 (Mouse reticulum sarcoma cell line) cells by Flow Cytometry (Intracellular).
Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells.
Antibody: Standard version of ab314044 (shaded red) or isotype control (unshaded).
Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.