AB130422

Anti-CD79b antibody [CB3-1]

Recombinant
What is this?

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(2 Publications)

Mouse Recombinant Monoclonal CD79b antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CD79b, B29, IGB, CD79B, B-cell antigen receptor complex-associated protein beta chain, B-cell-specific glycoprotein B29, Ig-beta, Immunoglobulin-associated B29 protein

2 Images

Flow Cytometry - Anti-CD79b antibody [CB3-1] (AB130422)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD79b antibody [CB3-1] (AB130422)

Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cells labeling CD79b with ab130422 at 1/50 (10 μg/mL) dilution (Red) compared with an Isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Fc receptors were blocked with 20 μg/mL Human IgG for 15 minutes on ice. Primary antibody staining for 30 minutes on ice. Secondary antibody staining for 30 minutes on ice.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD79b antibody [CB3-1] (AB130422)

Flow cytometry staining of human PBMC for CD79b with ab130422 at 1/50 dilution (10 μg/mL) (right) or an isotype control (left). Fc receptors were blocked with 20 μg/mL Human IgG for 15 minutes on ice. Primary antibody staining for 30 minutes on ice. Secondary antibody staining for 30 minutes on ice.

Carrier free

Anti-CD79b antibody [CB3-1] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CB3-1

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

This product has switched from a hybridoma to recombinant production method on 27^th October 2020.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD79b also known as Ig-beta is a protein that plays a mechanical role as part of the B-cell antigen receptor complex. This protein with a mass of approximately 40 kDa is mainly expressed on the surface of B lymphocytes. It is an important component of the heterodimeric complex composed of CD79a (Ig-alpha) and CD79b which associates with membrane-bound immunoglobulins to form the B-cell receptor (BCR). CD79b is essential for transducing activation signals following antigen binding to the BCR.

Biological function summary

The primary role of CD79b within the immune system is to support signal transduction. Upon binding of an antigen to BCR on B cells CD79b as part of the BCR complex initiates signaling cascades which lead to B-cell activation proliferation and differentiation. These processes are critical for humoral immunity ensuring adequate antibody production. CD79b is often found in association with CD19 another important B-cell surface protein and together they aid in amplifying the signals required for B-cell response.

Pathways

CD79b is deeply involved in the B-cell receptor signaling pathway and the broader adaptive immune response. Once the BCR is engaged by an antigen CD79b helps activate intracellular signaling pathways that involve kinases such as SYK and BLNK which help propagate and amplify the immune response. These pathways also interact with other receptor components like CD22 and CD19 creating a network of interactions ensuring effective pathogen recognition and response.

CD79b carries significant relevance in the context of B-cell malignancies such as chronic lymphocytic leukemia (CLL) and some types of lymphoma. Dysregulation or mutations in CD79b can affect BCR signaling leading to unchecked B-cell proliferation characteristic of these diseases. Additionally CD79b's interaction with proteins such as SYK in the signaling pathways makes it a potential therapeutic target in treating B-cell related cancers. Researchers are actively investigating anti-CD79b therapies to disrupt abnormal signaling in B-cell malignancies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.

See full target information CD79B

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical pathology 55:180-3 PubMed11896067

2002

New additions to antibody panels in the characterisation of chronic lymphoproliferative disorders.

Applications

Unspecified application

Species

Unspecified reactive species

E Matutes

Proceedings of the National Academy of Sciences of the United States of America 89:8522-6 PubMed1382292

1992

Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

T Nakamura,H Kubagawa,M D Cooper

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD79b antibody [CB3-1]

Flow Cytometry - Anti-CD79b antibody [CB3-1] (AB130422)

Flow Cytometry - Anti-CD79b antibody [CB3-1] (AB130422)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Sample Prep & Detection Kits

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise