Name: Anti-CD79b antibody [EPR6861]
SKU: ab134147
Rating: 2 (1 reviews)

Anti-CD79b antibody [EPR6861] (ab134147) is a rabbit monoclonal antibody that is used to detect CD79b in Western Blot, Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.

- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

Related conjugates and formulations

ab240083
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Anti-CD79b antibody [EPR6861] - BSA and Azide free
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PE Anti-CD79b antibody [EPR6861]
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APC Anti-CD79b antibody [EPR6861]
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HRP Anti-CD79b antibody [EPR6861]
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Alexa Fluor® 647 Anti-CD79b antibody [EPR6861]
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Alexa Fluor® 594 Anti-CD79b antibody [EPR6861]
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Alexa Fluor® 568 Anti-CD79b antibody [EPR6861]
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Alexa Fluor® 555 Anti-CD79b antibody [EPR6861]
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Alexa Fluor® 488 Anti-CD79b antibody [EPR6861]
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Alexa Fluor® 750 Anti-CD79b antibody [EPR6861]

Images

Flow Cytometry - Anti-CD79b antibody [EPR6861] (AB134147), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	Flow Cyt	WB	ICC/IF
Human	Tested	Tested	Tested	Tested
Mouse	Tested	Expected	Tested	Expected
Rat	Tested	Expected	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes For unpurified use at 1/250 - 1/500. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Human	Dilution info 1/500	Notes For unpurified use at 1/250 - 1/500. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500	Notes For unpurified use at 1/250 - 1/500. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/20	Notes For unpurified use at 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info 1/20	Notes For unpurified use at 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Species Rat	Dilution info 1/20	Notes For unpurified use at 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes For unpurified use at 1/1000 - 1/10000.
Species Human	Dilution info 1/1000	Notes For unpurified use at 1/1000 - 1/10000.
Species Rat	Dilution info 1/1000	Notes For unpurified use at 1/1000 - 1/10000.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes For unpurified use at 1/250 - 1/500.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50	Notes For unpurified use at 1/250 - 1/500.
Species Rat	Dilution info 1/50	Notes For unpurified use at 1/250 - 1/500.

Associated Products

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Target data

See full target information CD79B

Function

Required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.

Alternative names

CD79b, B29, IGB, CD79B, B-cell antigen receptor complex-associated protein beta chain, B-cell-specific glycoprotein B29, Ig-beta, Immunoglobulin-associated B29 protein

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR6861
Purification technique: Affinity purification Protein A
Dissociation constant: 4.7 x 10^-11 M
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-CD79b antibody [EPR6861] (ab134147) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of CD79b?

Anti-CD79b [EPR6861] (ab134147) specifically detects a band for CD79b (UniProt: P40259) at a molecular weight of 26kDa.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products

We have a range of other formats of antibody clone [EPR6861] also available for your convenience:
ab134147, Carrier free - ab240083, PE - ab305961, APC - ab305962, HRP - ab305963, Alkaline Phosphatase - ab308976, Alexa Fluor® 488 - ab309920, Alexa Fluor® 647 - ab310288, Alexa Fluor® 594 - ab310749, Alexa Fluor® 555 - ab312276, Alexa Fluor® 568 - ab312770, Alexa Fluor® 750 - ab321079

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD79b also known as Ig-beta is a protein that plays a mechanical role as part of the B-cell antigen receptor complex. This protein with a mass of approximately 40 kDa is mainly expressed on the surface of B lymphocytes. It is an important component of the heterodimeric complex composed of CD79a (Ig-alpha) and CD79b which associates with membrane-bound immunoglobulins to form the B-cell receptor (BCR). CD79b is essential for transducing activation signals following antigen binding to the BCR.

Biological function summary

The primary role of CD79b within the immune system is to support signal transduction. Upon binding of an antigen to BCR on B cells CD79b as part of the BCR complex initiates signaling cascades which lead to B-cell activation proliferation and differentiation. These processes are critical for humoral immunity ensuring adequate antibody production. CD79b is often found in association with CD19 another important B-cell surface protein and together they aid in amplifying the signals required for B-cell response.

Pathways

CD79b is deeply involved in the B-cell receptor signaling pathway and the broader adaptive immune response. Once the BCR is engaged by an antigen CD79b helps activate intracellular signaling pathways that involve kinases such as SYK and BLNK which help propagate and amplify the immune response. These pathways also interact with other receptor components like CD22 and CD19 creating a network of interactions ensuring effective pathogen recognition and response.

Associated diseases and disorders

CD79b carries significant relevance in the context of B-cell malignancies such as chronic lymphocytic leukemia (CLL) and some types of lymphoma. Dysregulation or mutations in CD79b can affect BCR signaling leading to unchecked B-cell proliferation characteristic of these diseases. Additionally CD79b's interaction with proteins such as SYK in the signaling pathways makes it a potential therapeutic target in treating B-cell related cancers. Researchers are actively investigating anti-CD79b therapies to disrupt abnormal signaling in B-cell malignancies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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8 product images

Flow Cytometry - Anti-CD79b antibody [EPR6861] (ab134147)
Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labelling CD79b with purified ab134147 at 1/20 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-CD79b antibody [EPR6861] (ab134147)
All lanes: Western blot - Anti-CD79b antibody [EPR6861] (ab134147) at 1/1000 dilution
Lane 1: Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2: Mouse spleen lysate
Lane 3: Rat spleen lysate
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 26 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] (ab134147)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD79b with purified ab134147 at 1/500 dilution (0.20 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Immunocytochemistry/ Immunofluorescence - Anti-CD79b antibody [EPR6861] (ab134147)
Immunocytochemistry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells labeling CD79b with purified ab134147 at 1/50 dilution (2.04 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] (ab134147)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue sections labeling CD79b with purified ab134147 at 1/500 dilution (0.20 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [EPR6861] (ab134147)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue sections labeling CD79b with purified ab134147 at 1/500 dilution (0.20 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
OI-RD Scanning - Anti-CD79b antibody [EPR6861] (ab134147)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Western blot - Anti-CD79b antibody [EPR6861] (ab134147)
False colour image of Western blot: Anti-CD79b antibody [EPR6861] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab134147 was shown to bind specifically to CD79b. A band was observed at 39 kDa in wild-type Raji cell lysates with no signal observed at this size in CD79B CRISPR-Cas9 edited cell line Human CD79B knockout Raji cell line ab273839 (CRISPR-Cas9 edited cell lysate ab273793). The band observed in the CRISPR-Cas9 edited lysate lane below 39 kDa is likely to represent a truncated form of CD79b. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and CD79B CRISPR-Cas9 edited Raji cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-CD79b antibody [EPR6861] (ab134147) at 1/1000 dilution
Lane 1: Wild-type Raji cell lysate at 20 µg
Lane 2: CD79B CRISPR-Cas9 edited Raji cell lysate at 20 µg
Lane 3: Daudi cell lysate at 20 µg
Lane 4: HAP1 cell lysate at 20 µg
Performed under reducing conditions.