Anti-CD79b antibody [SP240] - BSA and Azide free

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD79b antibody [SP240] - BSA and Azide free (AB245745)

Immunocytochemistry/ Immunofluorescence analysis of Daudi (human Burkitt's lymphoma lymphoblast) cells labeling CD79b with purified ab183343 at 1/50 (4.12 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183343).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CD79b antibody [SP240] - BSA and Azide free (AB245745)

Intracellular Flow Cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells labeling CD79b with purified ab183343 at 1/200 dilution (1.05μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183343).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79b antibody [SP240] - BSA and Azide free (AB245745)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD79b with ab183343 at 1/100 (0.11 μg/ml) followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab183343 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins was used.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183343).

• WB

Supplier Data

Western blot - Anti-CD79b antibody [SP240] - BSA and Azide free (AB245745)

This image was generated using the same antibody clone from a different batch.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183343).

All lanes:

Western blot - Anti-CD79b antibody [SP240] (<a href='/en-us/products/primary-antibodies/cd79b-antibody-sp240-ab183343'>ab183343</a>) at 1/100 dilution

All lanes:

Daudi Cell Lysate

Predicted band size: 26 kDa

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