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AB25478

Anti-CD8 alpha antibody [53-6.7]

5

(2 Reviews)

|

(14 Publications)

Rat Monoclonal CD8 alpha antibody. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 14 publications.

View Alternative Names

CD8a, Lyt-2, Lyt2, Cd8a, T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2

1 Images
Flow Cytometry - Anti-CD8 alpha antibody [53-6.7] (AB25478)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD8 alpha antibody [53-6.7] (AB25478)

Flow Cytometry analysis of BALB/c mouse splenocytes labeling CD8 alpha with ab25478 at 1 μg/106 cells, and Rat Anti-Mouse CD4-UNLB, followed by Mouse Anti-Rat IgG2a-AF647 and Mouse Anti-Rat IgG2b-AF488.

  • 578 PE

    PE Anti-CD8 alpha antibody [53-6.7]

  • 667 Cy5®

    Cy5 ® Anti-CD8 alpha antibody [53-6.7]

  • 695 APC/Cy5.5®

    APC/Cy5.5® Anti-CD8 alpha antibody [53-6.7]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD8 alpha antibody [53-6.7]

  • 519 FITC

    FITC Anti-CD8 alpha antibody [53-6.7]

  • 578 PE

    PE Anti-CD8 alpha antibody [53-6.7]

  • 660 APC

    APC Anti-CD8 alpha antibody [53-6.7]

  • Biotin

    Biotin Anti-CD8 alpha antibody [53-6.7]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

53-6.7

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Specificity

ab25478 recognises a chain of the murine CD8 heterodimer.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18450'>ab18450</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

This antibody blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 8.2 Constituents: 100% Borate buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD8 alpha also known as CD8A or CD8 protein is a glycoprotein subunit expressed on the surface of cytotoxic T lymphocytes. It has a mass of approximately 32 kDa. Found on the surface cell membrane CD8 alpha functions primarily in the immune response specifically in the recognition of antigens bound to major histocompatibility complex (MHC) class I molecules. Often scientists use CD8 antibodies for detection and CD8 IHC or immunohistochemistry for localization studies.
Biological function summary

The CD8 alpha protein plays a critical role in T-cell mediated immune responses. It forms a heterodimer with the CD8 beta chain creating the CD8 alpha-beta complex that strengthens T-cell interaction with antigen-presenting cells. CD8 alpha also helps in signaling processes that activate T cells equipping them to destroy infected or malignant cells. Researchers often study CD8 alpha peptides to understand its interactions better.

Pathways

CD8 alpha is integral to the T-cell receptor signaling pathway and the cytotoxic T lymphocyte (CTL) pathway. The T-cell receptor complex which includes the CD8 molecule transmits signals that are important for T-cell activation and function. CD8 interacts with key proteins such as the T-cell receptor (TCR) and MHC class I molecules facilitating targeted responses against pathogens. These pathways highlight CD8 alpha’s role in adaptive immunity.

CD8 alpha is most prominently associated with viral infections and cancer. Conditions like HIV and some forms of leukemia show altered CD8 function highlighting the protein's role in immune surveillance. In HIV infection for instance CD8 T cells reduce in number impairing the immune response. CD8 alpha’s connection to the immune system places it alongside other immune proteins such as CD4 and MHC molecules in the context of immune dysfunction.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule : peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
See full target information Cd8a
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy : the journal of the American Society of Gene Therapy 30:3552-3569 PubMed35821634

2022

IL-15 blockade and rapamycin rescue multifactorial loss of factor VIII from AAV-transduced hepatocytes in hemophilia A mice.

Applications

Unspecified application

Species

Unspecified reactive species

John S S Butterfield,Kentaro Yamada,Thais B Bertolini,Farooq Syed,Sandeep R P Kumar,Xin Li,Sreevani Arisa,Annie R Piñeros,Alejandro Tapia,Christopher A Rogers,Ning Li,Jyoti Rana,Moanaro Biswas,Cox Terhorst,Randal J Kaufman,Ype P de Jong,Roland W Herzog

Infection and immunity 90:e0038221 PubMed34780279

2021

Incomplete Recruitment of Protective T Cells Is Associated with Trypanosoma cruzi Persistence in the Mouse Colon.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander I Ward,Michael D Lewis,Martin C Taylor,John M Kelly

International immunology 33:211-224 PubMed33119735

2020

Psoriasis-like skin disorder in transgenic mice expressing a RIG-I Singleton-Merten syndrome variant.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed Abu Tayeh,Masahide Funabiki,Shota Shimizu,Saya Satoh,Lee Sumin,Yoichiro Iwakura,Hiroki Kato,Takashi Fujita

European journal of immunology 50:2041-2054 PubMed32640051

2020

Cross-presentation of dead-cell-associated antigens by DNGR-1 dendritic cells contributes to chronic allograft rejection in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Saidou Balam,Rebecca Kesselring,Elke Eggenhofer,Stephanie Blaimer,Katja Evert,Matthias Evert,Hans J Schlitt,Edward K Geissler,Janneke van Blijswijk,Sonia Lee,Caetano Reis e Sousa,Stefan M Brunner,Stefan Fichtner-Feigl

Scientific reports 9:4712 PubMed30886310

2019

RNAi-Mediated PD-L1 Inhibition for Pancreatic Cancer Immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Byunghee Yoo,Veronica Clavijo Jordan,Patrick Sheedy,Ann-Marie Billig,Alana Ross,Pamela Pantazopoulos,Zdravka Medarova

The Journal of experimental medicine 216:556-570 PubMed30787033

2019

A major role for ferroptosis in -induced cell death and tissue necrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Eduardo P Amaral,Diego L Costa,Sivaranjani Namasivayam,Nicolas Riteau,Olena Kamenyeva,Lara Mittereder,Katrin D Mayer-Barber,Bruno B Andrade,Alan Sher

Oxidative medicine and cellular longevity 2017:9303054 PubMed29456788

2017

Antroquinonol Exerts Immunosuppressive Effect on CD8 T Cell Proliferation and Activation to Resist Depigmentation Induced by HO.

Applications

Unspecified application

Species

Unspecified reactive species

Cuiping Guan,Qingtian Li,Xiuzu Song,Wen Xu,Liuyu Li,Aie Xu

PloS one 12:e0175300 PubMed28448494

2017

Integrin β1 activation induces an anti-melanoma host response.

Applications

ICC/IF, ICC/IF, IHC, IHC

Species

Mouse, Human, Human, Mouse

Laila Ritsma,Ipsita Dey-Guha,Nilesh Talele,Xavier Sole, Salony,Joeeta Chowdhury,Kenneth N Ross,Sridhar Ramaswamy

The American journal of pathology 187:1368-1379 PubMed28427861

2017

Divergent Function of Programmed Death-Ligand 1 in Donor Tissue versus Recipient Immune System in a Murine Model of Bronchiolitis Obliterans.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Schütte-Nütgen,Olaf Boenisch,Hakima Harrach,Alicia Casey,Indira Guleria,Nader Najafian,Mohamed H Sayegh,Craig J Gerard,Meera Subramaniam

Nature communications 8:14003 PubMed28084302

2017

K48-linked KLF4 ubiquitination by E3 ligase Mule controls T-cell proliferation and cell cycle progression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenyue Hao,Yi Sheng,Gordon S Duncan,Wanda Y Li,Carmen Dominguez,Jennifer Sylvester,Yu-Wen Su,Gloria H Y Lin,Bryan E Snow,Dirk Brenner,Annick You-Ten,Jillian Haight,Satoshi Inoue,Andrew Wakeham,Alisha Elford,Sara Hamilton,Yi Liang,Juan C Zúñiga-Pflücker,Housheng Hansen He,Pamela S Ohashi,Tak W Mak
View all publications
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