AB316355

Anti-CD8 alpha antibody [Bu88]

Name: Anti-CD8 alpha antibody [Bu88] (ab316355) | Abcam
Brand: Abcam Limited
SKU: AB316355

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Mouse Monoclonal CD8 alpha antibody. Suitable for ICC/IF, Flow Cyt, WB and reacts with Human samples. Immunogen corresponding to Native Full Length Protein corresponding to Human CD8A.

View Alternative Names

CD8a, MAL, CD8A, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-CD8 alpha Antibody [Bu88] (AB316355)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD8 alpha Antibody [Bu88] (AB316355)

Immunofluorescence analysis of paraformaldehyde fixed Jurkat (Human T cell leukemia cell line from peripheral blood) cells. Primary incubation 1hr (1 : 50-1 : 100 dilution) followed by Alexa Fluor® 488 secondary antibody (1 : 1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Isotype control : Anti-Fluorescein followed by Alexa Fluor® 488 secondary antibody.

Flow Cytometry - Anti-CD8 alpha Antibody [Bu88] (AB316355)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD8 alpha Antibody [Bu88] (AB316355)

Blue line : Flow cytometric analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells. Primary incubation 1hr (1 : 50-1 : 100 dilution) followed by Alexa Fluor 488 ® conjugated goat Anti-mouse IgG (1 : 1000 dilution). Black line : Anti-Unknown Specificity Isotype control.

Supplier Data

Western blot - Anti-CD8 alpha Antibody [Bu88] (AB316355)

(1 µg/ml) staining in MOLT-4 (Human lymphoblastic leukemia cell line) lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-CD8 alpha Antibody [Bu88] (ab316355) at 1 µg/mL

All lanes:

MOLT-4 cell lysate

true

Exposure time: 1hr

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Bu88

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt, ICC/IF

applications

Immunogen

Native Full Length Protein corresponding to Human CD8A.

P01732

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50 - 1/100", "FlowCyt-species-notes": "(Unfixed cells)", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1 month

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD8 alpha also known as CD8A or CD8 protein is a glycoprotein subunit expressed on the surface of cytotoxic T lymphocytes. It has a mass of approximately 32 kDa. Found on the surface cell membrane CD8 alpha functions primarily in the immune response specifically in the recognition of antigens bound to major histocompatibility complex (MHC) class I molecules. Often scientists use CD8 antibodies for detection and CD8 IHC or immunohistochemistry for localization studies.

Biological function summary

The CD8 alpha protein plays a critical role in T-cell mediated immune responses. It forms a heterodimer with the CD8 beta chain creating the CD8 alpha-beta complex that strengthens T-cell interaction with antigen-presenting cells. CD8 alpha also helps in signaling processes that activate T cells equipping them to destroy infected or malignant cells. Researchers often study CD8 alpha peptides to understand its interactions better.

Pathways

CD8 alpha is integral to the T-cell receptor signaling pathway and the cytotoxic T lymphocyte (CTL) pathway. The T-cell receptor complex which includes the CD8 molecule transmits signals that are important for T-cell activation and function. CD8 interacts with key proteins such as the T-cell receptor (TCR) and MHC class I molecules facilitating targeted responses against pathogens. These pathways highlight CD8 alpha’s role in adaptive immunity.

CD8 alpha is most prominently associated with viral infections and cancer. Conditions like HIV and some forms of leukemia show altered CD8 function highlighting the protein's role in immune surveillance. In HIV infection for instance CD8 T cells reduce in number impairing the immune response. CD8 alpha’s connection to the immune system places it alongside other immune proteins such as CD4 and MHC molecules in the context of immune dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the gene CD8A is an integral membrane glycoprotein crucial for immune responses, operating mainly in T-cells as a coreceptor for MHC class I molecule : peptide complexes. These peptides originate from cytosolic proteins, unlike class II peptides, which are from extracellular proteins. CD8A interacts with the T-cell receptor (TCR) and MHC class I proteins on antigen-presenting cells, recruiting the Src kinase LCK to the TCR-CD3 complex. LCK phosphorylates substrates, triggering signaling pathways that lead to the production of lymphokines, enhanced motility, adhesion, and activation of cytotoxic T-lymphocytes (CTLs), thereby aiding in the recognition and elimination of infected or tumor cells. Additionally, in natural killer (NK) cells, CD8A homodimers on the cell surface provide a survival mechanism for conjugating with and lysing multiple target cells. CD8A homodimers also facilitate the survival and differentiation of activated lymphocytes into memory CD8 T-cells. This supplementary information is collated from multiple sources and compiled automatically.

See full target information CD8A

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD8 alpha antibody [Bu88]

Immunocytochemistry/ Immunofluorescence - Anti-CD8 alpha Antibody [Bu88] (AB316355)

Flow Cytometry - Anti-CD8 alpha Antibody [Bu88] (AB316355)

Western blot - Anti-CD8 alpha Antibody [Bu88] (AB316355)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise